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Dear Zdenek,
The behaviour of the Borealis on your W1 sounds like there is something wrong. Even though we no longer supply Borealis for CSU products, because we integrate it into our own Dragonfly solution, we can still support the unit we sold to you. If you have not already been in contact our support team will reach out to you to see if we can better understand the conditions and so find the root cause of your problem.
As it stands, the larger fields of view that some microscopes now offer do not mean that loss of field size with optical super-res add-ons can be reversed. It is the field size limits after the side-port of the microscope that determines this. The extent of loss of field of view varies from solution to solution and needs considering accordingly
Kind regards
Geraint
Geraint Wilde Ph.D.
Product Manager - Microscopy Systems Division
Andor Technology
Tel: +44 (0) 7841 051633
https://andor.oxinst.com-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Emmanuel Levy
Sent: 21 February 2019 09:43
To:
[hidden email]
Subject: -|EXT|- Re: [CONFOCALMICROSCOPY] Yokogawa W1 spinning disk with OPRA-style superresolution
Hi,
I have no experience with the SORA add-on. One thing to keep in mind is
that it decreases the field of view by 75% (because of the 2x
magnification).
On a different note, anyone familiar with the Visitron's beam homogenizing
> system for CSU-W1 willing to share some details? How does it compare to
> "Andor's" Borealis? The thing is that we're observing some strange
> borealis-related effects at short exposures; moreover, Andor does not sell
> W1 with borealis anymore...
We've had the homogenizer for a while. The homogeneity is better than with
regular W1 optics but not flat. In our system we get ~10-15% variation
across the FOV for GFP, 20-25% for RFP, and about or above 30% for BFP.
Hope this helps,
Emmanuel
>
> Thanks!
>
> Best, zdenek
> --
> --
> Zdenek Svindrych, Ph.D.
> Research Associate - Imaging Specialist
> Department of Biochemistry and Cell Biology
> Geisel School of Medicine at Dartmouth
>
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