Re: batch processing of Olympus .oib/.tif files

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Jerry (Gerald) Sedgewick Jerry (Gerald) Sedgewick
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Re: batch processing of Olympus .oib/.tif files

In our core facility we have had a number of people who amass a lot of Olympus .oib (Fluoview 1000) and .tif (Fluoview 300/500) images and would like to batch process an entire directory of images.  Generally, they would like to 1) z-project each channel using the maximum projection and then 2) save the projections from each channel to a single-plane, projected .tif file (e.g., if 3 channels of z-planes were collected then 3 .tif files would result for each channel) automatically to an entire directory.

Our computer expert (Gregg Amundson) wrote a plug-in for Image J available on our web page in the Download section (http://www.bipl.umn.edu) to solve this problem.  Feel free to download.  If something other than a max projection is desired, it's fairly easy to change that line in the code. 

This hasn't been tested yet on Vista.

Jerry Sedgewick
University of Minnesota
Tao Tong Tao Tong
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Re: batch processing of Olympus .oib/.tif files

I wrote a similar script (journal) in MetaMorph to do similar stuff. It is easier in MetaMorph since it can read the Olympus data sets directly and simple to do projections and other stuff.

Tao

On Fri, Oct 23, 2009 at 3:40 PM, Jerry (Gerald) Sedgewick <[hidden email]> wrote:
In our core facility we have had a number of people who amass a lot of Olympus .oib (Fluoview 1000) and .tif (Fluoview 300/500) images and would like to batch process an entire directory of images.  Generally, they would like to 1) z-project each channel using the maximum projection and then 2) save the projections from each channel to a single-plane, projected .tif file (e.g., if 3 channels of z-planes were collected then 3 .tif files would result for each channel) automatically to an entire directory.

Our computer expert (Gregg Amundson) wrote a plug-in for Image J available on our web page in the Download section (http://www.bipl.umn.edu) to solve this problem.  Feel free to download.  If something other than a max projection is desired, it's fairly easy to change that line in the code. 

This hasn't been tested yet on Vista.

Jerry Sedgewick
University of Minnesota



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