Re: chambers with silicone
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Evangelos-
I have been told that there are chambers with silicone seals, and you may enter the sealed ring with 22 size syringe needles to make additions and/or release air bubbles from the sealed chambers. Bellco www.bellcoglass.com tel. 800.257.7043 fax. 856.691.3247 [hidden email] Description Wrench for Assembly of Sykes-Moore Culture Chamber Part # 1943-44444 Description Cover Slip Round #1 Thickness, 25mm Part # 1943-22222 Description Gasket for Sykes Moore Chamber Red Silicone with 2.5mm Working Distance Part # 1943-33325 Description Gasket Red Silicone for Sykes Moore Chamber, 25mm Part # 1943-22115 Description Sykes-Moore Culture Chamber Only, SST Part # 1943-11111 Description Sykes-Moore Chamber Holder for Microscope Viewing, Aluminum 3 x 1-1/2 x 3/16" Part # 1943-55555 ________________________________________ From: Confocal Microscopy List [[hidden email]] On Behalf Of Evangelos [[hidden email]] Sent: Wednesday, June 10, 2009 1:25 PM To: [hidden email] Subject: chambers for volatile organics I've been working lately on confocal microscopy with very volatile and toxic organics. The chambers described today are quite useful (Matek...) for cell culture and cell imaging; however, the solvents I've been working with lately readily attack plastic and rubber. I would need a chamber made of glass or fused silica, with a thickness at the bottom comparable to a 0, 1, or 1.5 standard microscope coverslip for an inverted microscope, and Luer Locks or similar pipette access ports made of Teflon or with Teflon plugs. I haven't found a supplier yet, if any vendor here or any of you know of a vendor that makes any type of chamber with glass only and with PTFE plugs that would be terrific. Best, Evangelos Harvard CNS |
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WillcoWells www.willcowells.com have a self assembly kit. This would allow you to assemble the coverslip into a plastic 35mm dish with the sealant of your choice. That is assuming the organics would not eat the 35mm plastic dish.
Tom -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]]On Behalf Of Carol Heckman Sent: Wednesday, June 10, 2009 12:46 PM To: [hidden email] Subject: Re: chambers with silicone Evangelos- I have been told that there are chambers with silicone seals, and you may enter the sealed ring with 22 size syringe needles to make additions and/or release air bubbles from the sealed chambers. Bellco www.bellcoglass.com tel. 800.257.7043 fax. 856.691.3247 [hidden email] Description Wrench for Assembly of Sykes-Moore Culture Chamber Part # 1943-44444 Description Cover Slip Round #1 Thickness, 25mm Part # 1943-22222 Description Gasket for Sykes Moore Chamber Red Silicone with 2.5mm Working Distance Part # 1943-33325 Description Gasket Red Silicone for Sykes Moore Chamber, 25mm Part # 1943-22115 Description Sykes-Moore Culture Chamber Only, SST Part # 1943-11111 Description Sykes-Moore Chamber Holder for Microscope Viewing, Aluminum 3 x 1-1/2 x 3/16" Part # 1943-55555 ________________________________________ From: Confocal Microscopy List [[hidden email]] On Behalf Of Evangelos [[hidden email]] Sent: Wednesday, June 10, 2009 1:25 PM To: [hidden email] Subject: chambers for volatile organics I've been working lately on confocal microscopy with very volatile and toxic organics. The chambers described today are quite useful (Matek...) for cell culture and cell imaging; however, the solvents I've been working with lately readily attack plastic and rubber. I would need a chamber made of glass or fused silica, with a thickness at the bottom comparable to a 0, 1, or 1.5 standard microscope coverslip for an inverted microscope, and Luer Locks or similar pipette access ports made of Teflon or with Teflon plugs. I haven't found a supplier yet, if any vendor here or any of you know of a vendor that makes any type of chamber with glass only and with PTFE plugs that would be terrific. Best, Evangelos Harvard CNS |
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In reply to this post by Carol Heckman
Dear Evangelos,
I have extensively used these Sykes-Moore chambers in both open and sealed modalities, although not with solvents (other than water). If you think a silicone o-ring will withstand your solvents I think these will do the trick nicely.
And as Carol mentioned you can push hypodermics through the o-ring to create a very simple flow bath.
You probably DON'T need the gasket Part # 1943-22115, this is the flat thin washer that is not suitable for poking a hypodermic through.
See Fig. 3 in the paper:
John A. Sykes (1978), "Sykes Moore Chamber"
Methods in Cell Science, Volume 4, Number 2 / June
No commercial affiliation with Bellco.
All the best,
Steve
Stephen H. Cody
Imaging Research Fellow & Manager Monash Micro Imaging – AMREP Monash University 6 Floor Burnet Tower Alfred Medical Research & Education Precinct 89 Commercial Rd, Melbourne, Australia, 3004 www.microimaging.monash.org 2009/6/11 Carol Heckman <[hidden email]>
Evangelos-
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