Re: coverslips for cell culture (chambers on on plastic slides)

[Shalin Mehta]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi all,

 

 I was looking for a low profile chamber made up of two coverslips. Ian Dobbie earlier suggested drilling holes in standard glass slides and then  making a chamber by placing coverslip on both sides as a quick solution. I think it may be easier and (safer) to do it with plastic slides - better if they are autoclavable. Any good sources of plastic autoclavable slides with low autofluorescnce?

 

Cheers

Shalin

 

On 10/27/07, Claire Brown <[hidden email]> wrote:

Search the CONFOCAL archive at
<a onclick="return top.js.OpenExtLink(window,event,this)" href="http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal" target="_blank"> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

We use 35 mm glass bottom dishes from World Precision Instruments. They say
on their web page they are 0.17 mm +- 0.01 mm. They are also much cheaper
than other suppliers if you buy them in boxes of 100.

No commercial interest.

Claire

--
My co-ordinates:
Shalin Mehta, Graduate student
Graduate Programme in Bioengineering, NUS, Singapore
Mobile: [phone]

[Sambra Redick]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Shalin,

For a non-autofluorescent, autoclavable, reusable solution, have your local machine shop make slide shaped blanks out of 1mm thick aluminum.  They can be drilled in whatever pattern you need.  Coverslips can be mounted with valap or vacuum grease.

Regards,

Sam

Sambra Redick

Program in Molecular Medicine

UMass Medical School

373 Plantation Street

206 Biotech 2

Worcester, MA 01605

On Oct 26, 2007, at 8:37 PM, Shalin Mehta wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi all,

 

 I was looking for a low profile chamber made up of two coverslips. Ian Dobbie earlier suggested drilling holes in standard glass slides and then  making a chamber by placing coverslip on both sides as a quick solution. I think it may be easier and (safer) to do it with plastic slides - better if they are autoclavable. Any good sources of plastic autoclavable slides with low autofluorescnce?

 

Cheers

Shalin