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Re: deconvolution software

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Ponti, Aaron

Re: deconvolution software

Dear all,

as Vincent points out, the Huygens software is indeed fully scriptable.
And we took advantage of this and wrote the Huygens Remote Manager, a
multi-user web interface to the Huygens Suite that allows for efficient,
parallel batch deconvolutions. Even better: the HRM is open source and
free (but you still need to buy a license for the backend Huygens Core
from SVI - http://www.svi.nl)!

Every user can set up an start deconvolution jobs remotely with a few
click from any recent web browser (from their laptop, for instance) and
go home (or do something else ;-) ). The HRM will inform him/her when
his jobs are done.

Please take a look at the web page of the project:
http://huygens-rm.org/

You can try a demo installation here: http://support.svi.nl/hrm/

For those of you who already know the HRM, on April 6th we released
version 1.2.1. The full changelog is here:
http://huygens-rm.org/wiki/index.php?title=Changelog

Since a couple of days there is even a new HRM mailing. You can
subscribe here: https://lists.sourceforge.net/lists/listinfo/hrm-list

Hope you will give it a try
Aaron

-----Original Message-----
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There are 14 messages totalling 1200 lines in this issue.

Topics of the day:

1. deconvolution software
2. Acceptor Photobleaching vs. Sensitized Emission FRET results (2)
3. Nikon Job Opportunity - Confocal Systems Specialist
4. Labs for graduate level microscopy course? (3)
5. Olympus FV1000 vs Nikon A1 (4)
6. Price of Olympus FV1000 and other systems
7. 488 nm 50mW diodes for old NORAN
8. Dupal, Mark is out of the office.

----------------------------------------------------------------------

Date: Wed, 31 Mar 2010 11:57:47 +0200
From: Vincent <[hidden email]>
Subject: Re: deconvolution software

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<pre>
*commercial interest*

</pre>
 
Hello, 
 
Thank you Stan for your nice overview. 
I would like to add the following to what you wrote about our Huygens
software. 
 
 
<blockquote cite="mid:LISTSERV%[hidden email]"
type="cite">
<pre wrap="">I believe Huygens allows full scripting, so processing
large number of stacks without
intervention should be possible. </pre>
</blockquote>
Both the Huygens Essential and Professional have a batch
processor to schedule and automate large numbers of deconvolution tasks
. 
 
The Huygens Professional, like the Scripting (a package that was not
mentioned), has indeed full Tcl-based scripting possibilities for
processing large file series. 
 
Then, we have also a web-based version which is the Huygens Core. The
Core can work with the Huygens Remote Manager (HRM; open source) as the
web-interface to schedule deconvolution tasks. 
 
 
<blockquote cite="mid:LISTSERV%[hidden email]"
type="cite">
<pre wrap="">On the other hand, there is no blind deconvolution
algorithm, you use either a theoretical or experimental point
spread function. Also, I do not remember if you can estimate spherical
aberration from the dataset itself, or if it is only calculated from
your
input parameters (RI of the mounting medium, RI of the immersion fluid,
depth of imaging).
</pre>
</blockquote>
 
Like the theoretical PSF, the spherical aberration (SA) is not
estimated from the image but based on the known microscopical and
sample parameters. 
 
We have recently added the possibility to set the distance of the first
image plane to the coverslip, since the SA progressively worsens when
imaging further from the coverslip. 
 
<blockquote cite="mid:LISTSERV%[hidden email]"
type="cite">
<pre wrap="">AutoDeblur and Huygens are modular, so the cost will
depend on exactly what
options you get. I would expect to pay between $6k and 10k for a
confocal-only package that does not include the scripting or surface
rendering options.
</pre>
</blockquote>
 
The Huygens Essential (32bit/64bit) with a confocal deconvolution
option and the
batch processor is available below this range. 
 
Best regards, 
 
Vincent Schoonderwoert 
 
<pre class="moz-signature"
cols="72">***********************************************************
Vincent Schoonderwoert, PhD
Imaging Specialist/Account Manager
Scientific Volume Imaging bv
Laapersveld 63
1213 VB Hilversum, The Netherlands
Tel: + 31 35 646 8216
Fax: + 31 35 683 7971
<a moz-do-not-send="true" class="moz-txt-link-abbreviated"
href="http://www.svi.nl">www.svi.nl</a>
***********************************************************

</pre>
 
<blockquote cite="mid:LISTSERV%[hidden email]"
type="cite">
<pre wrap="">

</pre>
<blockquote type="cite">
<pre wrap="">Hello all,

Our lab is considering purchasing some deconvolution software for
cleaning
up confocal image stacks. I'm soliciting any recommendations, pro or
con,
for particular software packages, based on quality of results, ease of
use,
cost etc. If you prefer to send your comments directly rather than post
them, please reach me at the email below.

Thanks very much for your consideration!

Cheers,

David Stuss

<a class="moz-txt-link-abbreviated"
href="mailto:[hidden email]">[hidden email]</a>

</pre>
</blockquote>
<pre wrap="">
</pre>
</blockquote>
 
 
<pre class="moz-signature" cols="72">--
***********************************************************
Vincent Schoonderwoert, PhD
Imaging Specialist/Account Manager
<a class="moz-txt-link-abbreviated"
href="mailto:[hidden email]">[hidden email]</a>

Scientific Volume Imaging bv
Laapersveld 63
1213 VB Hilversum, The Netherlands
Tel: + 31 35 646 8216
Fax: + 31 35 683 7971
<a class="moz-txt-link-abbreviated"
href="http://www.svi.nl">www.svi.nl</a>
<a class="moz-txt-link-abbreviated"
href="mailto:[hidden email]">[hidden email]</a>
***********************************************************
SVI Customer support: mail us your questions <a
class="moz-txt-link-abbreviated"
href="mailto:[hidden email]">[hidden email]</a>
or find answers online in our FAQ: <a class="moz-txt-link-freetext"
href="http://support.svi.nl">http://support.svi.nl</a>

</pre>
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Date: Wed, 31 Mar 2010 09:34:45 -0400
From: yuansheng sun <[hidden email]>
Subject: Re: Acceptor Photobleaching vs. Sensitized Emission FRET
results

Dear Pablo,

This is sheng, working at the Keck center, UVA. I think we have
contacted earlier for the new pFRET software. Here are my comments for
this topic:

I would not be surprised to see different FRET efficiencies for
different Donor : Acceptor ratios or different Acceptor levels. That
is actually a valuable indication for the random association or the
cluster assembly. I recommend you look at the following paper -
Biophys. J. Vol 85, Issue 1, 559-571 (2003).
If you write me an email ([hidden email]), I can send you a
couple of nice PPTs on this topic.

I like your strategy - measuring FRET of a same system in different
ways (SE vs. AP). If possible, I would like to try FLIM as well since
lifetime is independent of fluorophore concentration, only if
possible. We have to work with what we have. I would not make the
decision to accept AP and reject SE, because I do not see a reason why
AP can give you more accurate (quantitative) results than SE, if your
experiments were done properly.

There are actually some potential issues you may check for using AP. I
assume your measurements were done with live cells.

1. Check if the donor is also bleached during the photobleaching
process. Use the donor-alone specimen to check. The apFRET plugin in
the new pFRET software allows you correct for this issue.

2. Check if the acceptor is completely bleached. Take the pre- and
post- acceptor images. Your FRET efficiency is certainly influenced by
the left acceptor amount after photobleaching. The apFRET plugin in
the new pFRET software allows you address this issue.

3. Check if there is any cellular movement or focus change. Overlay
pre- and post- images in two different colors to see if you will have
a perfect overlay. If not, I suggest you run AP with fixed cells to
see you will also have homogeneous FRET efficiencies.

Please shoot me an email if you need help using the pFRET software to
check the issues mentioned above. Good luck.

Best regards,
sheng

On Tue, Mar 30, 2010 at 11:53 PM, Pablo German
<[hidden email]> wrote:

> Dear list members,
>
> I have been doing some FRET microscopy experiments on a
> 7-TransMembrane domain receptor tagged with either eCFP/eYFP at the
> different intra-cellular loops (ICL1, ICL2, and ICL3). I have tried
> the 9 different combinations (e.g ICL1-YFP + ICL1-CFP, ICL1-YFP +
> ICL2-CFP, etc) to see if I could detect any difference in FRET
> efficiency.
>
> I have anlyzed the images by both Sensitized Emission and Acceptor
> Photobleaching using the pFRET plugin on ImageJ developed at KCCI-UVa.
> The problem is the following: the results using Sensitized Emission
> give me significant differences between the different pairs but the
> results using APB give me no differences (all about 25% efficiency).
>
> I have the feeling that I should trust APB more than SE. I have
> noticed that, when using SE, the higher the difference in intensity
> between YFP and CFP, the higher the FRET efficiency.
>
> Has anyone had a similar experience? Which method of analysis should I

trust?

>
> Regards,
> Pablo
>
> --
> Pablo German
> PhD Candidate
>
> Plant and Food Research
> Private Bag 92169
> Auckland Mail Centre
> Auckland 1142
> New Zealand
> DDI: (09) 925-7107
> Mobile: 0210459406
>

------------------------------

Date: Wed, 31 Mar 2010 09:46:19 -0400
From: yuansheng sun <[hidden email]>
Subject: Re: Acceptor Photobleaching vs. Sensitized Emission FRET
results

Pablo,

I forgot to ask if the FRET efficiency you mentioned refers to the
average of the whole cell. I think it is more appreciate to do
quantitative comparisons in details, such as comparing the FRET
efficiencies of different pairs for the same Donor : Acceptor ratios
or the same acceptor levels. If you can write me more details, we can
further discuss about the data analysis strategy.

sheng

On Tue, Mar 30, 2010 at 11:53 PM, Pablo German
<[hidden email]> wrote:

trust?

------------------------------

Date: Wed, 31 Mar 2010 11:31:22 -0400
From: "Cacace Stephanie M." <[hidden email]>
Subject: Nikon Job Opportunity - Confocal Systems Specialist

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If success matters...

Nikon matters.

Today Nikon is a world-renowned brand, firmly established as a market
leader in optical instrumentation and the only microscope company to
manufacture its own glass, ensuring the very finest quality assurance
throughout production. With over 90 years expertise in the field, Nikon
has always been at the forefront of optical and technological
innovation, promoting creativity and trustworthiness as part of the
company's global mission statement.

We are currently looking for a Confocal Systems Specialist to support
all aspects of our microscopy Confocal imaging business including
customer service, sales, product support and service, hardware and
software troubleshooting, working directly with customers to help via
the phone or web and demonstrating and discussing customer applications
related to the equipment.. Candidate must be proficient in operating a
Confocal microscope and possess the complete skill sets necessary to
install, integrate, test and troubleshoot all aspects of Confocal system
operation including microscope and Confocal firmware and software
systems, as well as troubleshoot optical imaging systems within
designated Confocal products.

Ideal candidate must hold a B.S. degree in Cell Biology or Molecular
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quantitative microscopy, computer hardware and software systems and have
the ability to analyze, design and document customer service processes.
Candidate must be able to work independently as well as in a
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functions up to 20% (includes overnight travel)

To find out how you can become a vital part of our team, please send
your resume and/or CV to: Nikon, 1300 Walt Whitman Road, Melville, NY
11747, Attn: Stephanie Cacace, Employment Specialist, Human Resources;
E-mail: [hidden email] or Fax: 631-547-4025. Nikon is an EEO/AA
Employer - M/F/D/V.

Stephanie Cacace
Employment Specialist

Nikon Inc.
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Office: 631-547-4255
Fax: 631-547-4025
[hidden email] www.nikonusa.com
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matters…  &nb=
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>

<st1:PersonName
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Nikon is a world-renowned brand, firmly established as a market leader
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optical instrumentation and the only microscope company to manufacture
its =
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glass, ensuring the very finest quality assurance throughout production.
Wi=
th
over 90 years expertise in the field, Nikon has always been at the
forefron=
t of
optical and technological innovation, promoting creativity and
trustworthin=
ess
as part of the company's global mission
statement.<o:p></o:p>=


<o:p> </o:p>

We=
are
currently looking for a Confocal
Systems
Specialist to
support =
all
aspects of our microscopy Confocal imaging business including customer
serv=
ice,
sales, product support and service, hardware and software
troubleshooting, =
working
directly with customers to help via the phone or web and demonstrating
and
discussing customer applications related to the equipment.. Candidate
must =
be
proficient in operating a Confocal microscope and possess the complete
skill
sets necessary to install, integrate, test and troubleshoot all aspects
of
Confocal system operation including microscope and Confocal firmware and
software systems, as well as troubleshoot optical imaging systems within
designated Confocal products.  <o:p></o:p>

<o=
:p> </o:p>

Id=
eal
candidate must hold a B.S. degree in Cell Biology or Molecular Biology
along
with 5+ years applied knowledge of image analysis, quantitative
microscopy,=
computer
hardware and software systems and have the ability to analyze, design
and
document customer service processes.  Candidate must be able to
work
independently as well as in a cross-functional team setting and display
exc=
ellent
technical skills, verbal and written communication skills, time
management =
and
multitasking skills.  Maximum travel required to fulfill essential
fun=
ctions up
to 20% (includes overnight travel)<o:p></o:p>

<o:p> </o:p>

To=
find out
how you can become a vital part of our team, please send your resume
and/or=
CV
to: <st1:PersonName w:st=3D"on">Nikon</=
span></st1:PersonName>, <st1:address w:st=3D"on"><st1:Street
w:st=3D"o=
n">1300
Walt Whitman Road</st1:Street>, <st1:City
w:st=3D"on">Melville</st1:City>=
, <st1:State
w:st=3D"on">NY</st1:State> <st1:PostalCode
w:st=3D"on">11747</st1:PostalCo=
de></st1:address>,
Attn: Stephanie Cacace, Employment Specialist, Human Resources; E-mail:
job=
[hidden email]
or Fax: 631-547-4025.  <st1:PersonName
w:st=3D"on">Nikon</s=
t1:PersonName>
is an EEO/AA Employer – M/F/D/V.<o:p></o:p>

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colSpan=3D2><STRON=
G>Stephanie M=
Cacace</TD></TR><TR vAlign=3Dtop><TD colSpan=3D2>Employment
Specialist<=
/FONT></TD></TR><TR><TD colSpan=3D2><IMG height=3D4
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colSpan=3D2><=
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Whit=
man Road</TD></TR><TR><TD colSpan=3D2>Melville NY
11747-3064</TD></TR><TR>=
<TD colSpan=3D2></TD></TR><TR><TD width=3D140>Office: 631-547-4255</TD><TD></TD></TR><TR><T=
D>Fax:
631-54=
7-4025 </TD><TD> </TD></TR><TR><TD><A
href=3D"mailto:[hidden email]">[hidden email]=
et</A></TD><TD> </TD></TR><TR><TD><A
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ikonusa.com/">www.nikonusa.com</A></TD><TD> </TD></TR></
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------------------------------

Date: Wed, 31 Mar 2010 18:08:32 +0200
From: Patrick Van Oostveldt <[hidden email]>
Subject: Re: Labs for graduate level microscopy course?

Dear,

Plant material is easy to handle.
Fruits like apple of cherry tomato's can be images as whole. The =20
epidermal cells contain fluorescent chloroplasts and the tha vacuoles
=20
are frequently filled with antocyaan dyes.
Pollen grains are always succesfull and can be obtained from lily or
dafodil=
l

Bye

Patrick

Also in reflection they generate nice images of surface.

Patrick

Quoting "Paul Herzmark" <[hidden email]>:

> Steve Ruzin at UC Berkeley has a good one for rejection of out of
focus
> light.
>
> Focus on the stomata on a leaf epidermis. There are lots of
fluorescent
> chloroplasts in the guard cells surrounding the stomata. The confocal
will

> block the fluorescence from the deeper mesophyl layer and all the
> chloroplasts there.
>
>
>
> Paul Herzmark
> Specialist
> [hidden email]
>
> Department of Molecular and Cell Biology
> 479 Life Science Addition
> University of California, Berkeley
> Berkeley, CA 94720-3200
> (510) 643-9603
> (510) 643-9500 fax
>
>
> On Tue, Mar 30, 2010 at 1:30 PM, Kurt Thorn <[hidden email]>

wrote:
>
>> Hi All -
>>
>> I'm putting together some labs for a graduate level microscopy course
I'l=
l
>> be teaching in the next few months and I was wondering if people here
had
>> ideas or materials for confocal microscopy labs that they could
share. I=
'm
>> particularly interested in doing 1 or 2 3 labs comparing widefield /
>> laser-scanning confocal / spinning-disk confocal, with respect to
both
>> sensitivity and rejection of out-of-focus light. Labs are 3 hours
long a=
nd
>> targeted and mid-level graduate students who may or may not have
microsco=
py
>> experience.
>>
>> I've got some ideas on how to do some of this but I'm curious to hear
of
>> exercises that have worked well for you and would love write ups or
other
>> materials you could share.
>>
>> Thanks!
>> Kurt
>>
>

--=20
Dep. Moleculaire Biotechnologie
Coupure links 653
B 9000 GENT

tel 09 264 5969
fax 09 264 6219

------------------------------

Date: Wed, 31 Mar 2010 12:17:47 -0400
From: gradice <[hidden email]>
Subject: Re: Labs for graduate level microscopy course?

Douglas Murphy's book "Fundamentals of Light Microscopy and Electronic
Imaging" has some exercises and demos that I have used. Among these he
includes a "build your own confocal" demo that, although I have not
used it myself, would be a lot of fun and illustrate the pinhole effect.

On Mar 30, 2010, at 4:30 PM, Kurt Thorn wrote:

> Hi All -
>
> I'm putting together some labs for a graduate level microscopy
> course I'll be teaching in the next few months and I was wondering
> if people here had ideas or materials for confocal microscopy labs
> that they could share.

Gary Radice
Department of Biology
University of Richmond
Richmond VA 23173
804-289-8107

------------------------------

Date: Wed, 31 Mar 2010 08:53:09 -0800
From: mps23 <[hidden email]>
Subject: Re: Olympus FV1000 vs Nikon A1

Hello all,

We are currently going to purchase a new confocal, comparing the Olympus
FV1000 spectral, Leica SP5, Nikon A1 and Zeiss 710. As said in other
posts,
this is not a fair comparison since the FV1000 is approximately 10 years
old. If this is the case, is it appropriate that the FV1000 spectral is
being sold for $350,000? I understand the Zeiss 710 since it is new
technology and the Leica and Nikon as well. Any comments - or has
anyone
recently purchased a FV1000 spectral? I've asked our Rep for reference
list
- still waiting?

Thanks.
--
View this message in context:
http://n2.nabble.com/Olympus-FV1000-vs-Nikon-A1-tp4567055p4832055.html
Sent from the Confocal Microscopy List mailing list archive at
Nabble.com.

------------------------------

Date: Wed, 31 Mar 2010 09:04:32 -0800
From: mps23 <[hidden email]>
Subject: Re: Price of Olympus FV1000 and other systems

Did you buy your Olympus? And if so, might I ask the price - we are in
the
same position with the FV1000 being priced the same as a zeiss 710.
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------------------------------

Date: Wed, 31 Mar 2010 13:44:16 -0400
From: Julian Smith III <[hidden email]>
Subject: Re: Olympus FV1000 vs Nikon A1

We've had our FV1000 spectral for two years. We're a primarily
undergraduate teaching institution with a tiny Master's program, an
emphasis on undergraduate research, and some NIH/NSF funded faculty in
both biology and chemistry. We have about 1000hrs/year use, almost
entirely undergraduate students, either in-class or for research
projects. It's been very robust in use, service has been excellent, and

we still don't use all of the capabilities of the instrument, except in
lab exercises designed to teach those techniques to our undergrads.

When we demoed the instruments three years ago, the Zeiss 710 wasn't out

yet, and the Nikon wasn't stable during the demo--the Elements software
hadn't been integrated yet; it's MUCH more solid since, as I can attest

from using the Nikon A1 at the QFM course in 2008 (along with the Leica
SP5 and the Zeiss 710 ). During our demo, the FV1000 was basically
bulletproof and far easier for our relatively untrained students to
use. We had it in-house for two weeks, and asked to have it back for
another three before we made up our minds (this may unusually long for a

demo, but bear in mind that our primary users are undergrads who are
in-class much of the week, and our faculty are teaching 12-contact-hour
loads).

If I were managing a typical research university core facility, I would
demo the instruments in-house as they currently exist. Speaking only
from summer 2008, I found the Zeiss 710 spectacularly light-efficient,
and the Zen software is a huge improvement over that on the 510. The
Nikon is far easier to use and more stable (than before) now that it's
running under Elements. The Leica has a great user interface, with well

thought-out mechanical controls so one's hand isn't always on the
mouse. At that point, I personally liked the Zeiss best, but I only had

my hands on the instruments for part of a week.

Concerning price, I'll simply note that given the figure you quote
below, it appears that the price might be negotiable <g>.
Happy hunting,
Julian

mps23 wrote:
> Hello all,
>
> We are currently going to purchase a new confocal, comparing the
Olympus
> FV1000 spectral, Leica SP5, Nikon A1 and Zeiss 710. As said in other
posts,
> this is not a fair comparison since the FV1000 is approximately 10
years
> old. If this is the case, is it appropriate that the FV1000 spectral
is
> being sold for $350,000? I understand the Zeiss 710 since it is new
> technology and the Leica and Nikon as well. Any comments - or has
anyone
> recently purchased a FV1000 spectral? I've asked our Rep for
reference list
> - still waiting?
>
> Thanks.
>

--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC 29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)

------------------------------

Date: Wed, 31 Mar 2010 15:30:49 -0400
From: "Todd A. Clason" <[hidden email]>
Subject: 488 nm 50mW diodes for old NORAN

Greetings from Vermont,

We have an old, but very useful Noran/Prairie Technologies fast
scanning laser confocal with a 488 nm gas laser that dies like
clockwork every two years.

Does anyone have recommendations on good diode replacements?

Thank you!

Todd

-----------------------------------
Todd Clason
Director, COBRE Imaging and Physiology Core
Department of Anatomy and Neurobiology
College of Medicine, University of Vermont
E015 Given
89 Beaumont Ave.
Burlington, VT 05405
p. 802.656.0413
[hidden email]

------------------------------

Date: Wed, 31 Mar 2010 21:31:09 +0100
From: "Littlejohn, George" <[hidden email]>
Subject: Re: Labs for graduate level microscopy course?

--_000_9E67E4654C6D46A893FE061B1D634173exeteracuk_
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: quoted-printable

Hi Gary and list,

On a similar note, Jim Haseloff's cheaposcope pages (not to mention the
res=
t of his pages) must be well worth a look for eager microscopists!

http://www.plantsci.cam.ac.uk/Haseloff/imaging/cheaposcope/cheaposcope.h
tm

This is, of course, not a confocal microscope. But is great fun!

George Littlejohn
School of Biosciences,
University of Exeter,
Mezzanine Laboratory,
Geoffrey Pope Building,
Stocker Road,
EX4 4QD, UK
******************************
Tel: +44(0)1392 269170 (Lab.)
+44(0)1392 269297 (Office)
Fax: +44(0)1392 263434
E-mail: [hidden email]<mailto:[hidden email]>

On 31 Mar 2010, at 17:17, gradice wrote:

Douglas Murphy's book "Fundamentals of Light Microscopy and Electronic
Imaging" has some exercises and demos that I have used. Among these he
includes a "build your own confocal" demo that, although I have not
used it myself, would be a lot of fun and illustrate the pinhole effect.

On Mar 30, 2010, at 4:30 PM, Kurt Thorn wrote:

Hi All -

I'm putting together some labs for a graduate level microscopy
course I'll be teaching in the next few months and I was wondering
if people here had ideas or materials for confocal microscopy labs
that they could share.

Gary Radice
Department of Biology
University of Richmond
Richmond VA 23173
804-289-8107

--_000_9E67E4654C6D46A893FE061B1D634173exeteracuk_
Content-Type: text/html; charset="us-ascii"
Content-Transfer-Encoding: quoted-printable

<html><head></head><body style=3D"word-wrap: break-word;
-webkit-nbsp-mode:=
space; -webkit-line-break: after-white-space; "><div>Hi Gary and
list,</di=
v><div> </div><div>On a similar note, Jim Haseloff's cheaposcope
pages (=
not to mention the rest of his pages) must be well worth a look for
eager m=
icroscopists!</div><div> </div><div><a
href=3D"http://www.plantsci.cam.a=
c.uk/Haseloff/imaging/cheaposcope/cheaposcope.htm">http://www.plantsci.c
am.=
ac.uk/Haseloff/imaging/cheaposcope/cheaposcope.htm</a></div><div> </d
iv>=
<div>This is, of course, not a confocal microscope. But is great
fun!</div>=
 <div>
<div>George Littlejohn School of Biosciences, University of
Exeter,<b=
r>Mezzanine Laboratory, Geoffrey Pope Building, Stocker
Road, EX4 =
4QD, UK ****************************** Tel:  +44(0)1392
269170 (=
Lab.)        +44(0)1392 269297
(Offic=
e) Fax:  +44(0)1392 263434 E-mail:  <a
href=3D"mailto:G.R.L=
[hidden email]">[hidden email]</a></div>
</div>
 <div><div>On 31 Mar 2010, at 17:17, gradice wrote:</div> <blockquote type=3D"cite"><div>Douglas Murphy's
bo=
ok "Fundamentals of Light Microscopy and Electronic   Imaging"
has =
some exercises and demos that I have used. Among these he
  include=
s a "build your own confocal" demo that, although I have not
  used=
it myself, would be a lot of fun and illustrate the pinhole
effect. <br=
> On Mar 30, 2010, at 4:30 PM, Kurt Thorn wrote: <blockquote
type=
=3D"cite">Hi All - </blockquote><blockquote
type=3D"cite"> </blockquo=
te><blockquote type=3D"cite">I'm putting together some labs for a
graduate =
level microscopy   </blockquote><blockquote type=3D"cite">course
I'=
ll be teaching in the next few months and I was wondering
  </block=
quote><blockquote type=3D"cite">if people here had ideas or materials
for c=
onfocal microscopy labs   </blockquote><blockquote
type=3D"cite">th=
at they could share. </blockquote> Gary Radice Department of
Biolo=
gy University of Richmond Richmond VA
23173 804-289-8107 </div>=
</blockquote></div> </body></html>=

--_000_9E67E4654C6D46A893FE061B1D634173exeteracuk_--

------------------------------

Date: Wed, 31 Mar 2010 17:27:01 -0400
From: RICHARD BURRY <[hidden email]>
Subject: Re: Olympus FV1000 vs Nikon A1

This is a multi-part message in MIME format.

----8b8ce11283a25abc12c7
Content-Type: text/html; charset=us-ascii
Content-Disposition: inline
Content-Transfer-Encoding: quoted-printable

Dear M=2E P=2E Stein=3CBR=3E=26nbsp=3B=3CBR=3EYou did not say how the
co=
nfocal was configured=2E=26nbsp=3B Having just purchased a FV1000=2C
the=
price you mentioned must include a lot of stuff=2E=26nbsp=3B Have you
g=
otten comparable quotes for the=26nbsp=3Bexact
same=26nbsp=3Bconfocal=26=
nbsp=3Bfrom Leica=2C Zeiss and Nikon=3F=26nbsp=3B
=3CBR=3E=26nbsp=3B=3CB=
R=3EDick=3CBR=3E=3CBR=3E----- Original Message -----=3CBR=3EFrom=3A
mps2=
3 =26lt=3Bmpstein1=40ATT=2ENET=26gt=3B=3CBR=3EDate=3A Wednesday=2C
March=
31=2C 2010 1=3A03 pm=3CBR=3ESubject=3A Re=3A Olympus FV1000 vs Nikon
A1=
=3CBR=3ETo=3A
CONFOCALMICROSCOPY=40LISTS=2EUMN=2EEDU=3CBR=3E=3CBR=3E=26g=
t=3B Hello all=2C=3CBR=3E=26gt=3B =3CBR=3E=26gt=3B We are currently
goin=
g to purchase a new confocal=2C comparing the =3CBR=3E=26gt=3B
OlympusFV=
1000 spectral=2C Leica SP5=2C Nikon A1 and Zeiss 710=2E=26nbsp=3B
=3CBR=3E=
=26gt=3B As said in other posts=2C=3CBR=3E=26gt=3B this is not a fair
co=
mparison since the FV1000 is approximately =3CBR=3E=26gt=3B 10
years=3CB=
R=3E=26gt=3B old=2E=26nbsp=3B If this is the case=2C is it appropriate
t=
hat the =3CBR=3E=26gt=3B FV1000 spectral is=3CBR=3E=26gt=3B being sold
f=
or =24350=2C000=3F=26nbsp=3B I understand the Zeiss 710 since
=3CBR=3E=26=
gt=3B it is new=3CBR=3E=26gt=3B technology and the Leica and Nikon as
we=
ll=2E=26nbsp=3B Any comments - =3CBR=3E=26gt=3B or has
anyone=3CBR=3E=26=
gt=3B recently purchased a FV1000 spectral=3F=26nbsp=3B I=27ve asked
our=
Rep =3CBR=3E=26gt=3B for reference list=3CBR=3E=26gt=3B - still
waiting=
=3F=3CBR=3E=26gt=3B =3CBR=3E=26gt=3B Thanks=2E=3CBR=3E=26gt=3B --
=3CBR=3E=
=26gt=3B View this message in context=3A
http=3A//n2=2Enabble=2Ecom/Olym=
pus-=3CBR=3E=26gt=3B
FV1000-vs-Nikon-A1-tp4567055p4832055=2Ehtml=3CBR=3E=
=26gt=3B Sent from the Confocal Microscopy List mailing list archive at
=
=3CBR=3E=26gt=3B Nabble=2Ecom=2E=3CBR=3E=26gt=3B =3CBR=3E=26gt=3B --
=3C=
BR=3E=26gt=3B BEGIN-ANTISPAM-VOTING-LINKS=3CBR=3E=26gt=3B
--------------=
----------------------------------------=3CBR=3E=26gt=3B
=3CBR=3E=26gt=3B=
Teach CanIt if this mail (ID 1019668206) is spam=3A=3CBR=3E=26gt=3B
Spa=
m=3A=26nbsp=3B=26nbsp=3B=26nbsp=3B=26nbsp=3B=26nbsp=3B=26nbsp=3B=26nbsp=
3B=
=3CBR=3E=26gt=3B
https=3A//antispam=2Eosu=2Eedu/b=2Ephp=3Fi=3D101966820=
6=26amp=3Bm=3Da3c72729dd07=26amp=3Bc=3DsNot
spam=3A=26nbsp=3B=26nbsp=3B=26=
nbsp=3B
https=3A//antispam=2Eosu=2Eedu/b=2Ephp=3Fi=3D1019668206=26amp=3B=
m=3Da3c72729dd07=26amp=3Bc=3Dn=3CBR=3E=26gt=3B Forget vote=3A
=3CBR=3E=26=
gt=3B
https=3A//antispam=2Eosu=2Eedu/b=2Ephp=3Fi=3D1019668206=26amp=3Bm=3D=
a3c72729dd07=26amp=3Bc=3Df---=3CBR=3E=26gt=3B
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-------------------------=3CBR=3E=26gt=3B
END-ANTISPAM-VOTING-LINKS=3CBR=
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=3CBR=3EDepa=
rtment of Neuroscience=2C College of Medicine =3CBR=3ECampus Microscopy
=
and Imaging Facility=2C Director =3CBR=3EThe Ohio State University
=3CBR=
=3EAssociate Editor=2C Journal of Histochemistry and Cytochemistry
=3CBR=
=3E277 Biomedical Research Tower =3CBR=3E460 West Twelfth Avenue
=3CBR=3E=
Columbus=2C Ohio 43210 =3CBR=3EVoice 614=2E292=2E2814=26nbsp=3B Cell
614=
=2E638=2E3345=26nbsp=3B Fax 614=2E247=2E8849=3CBR=3E=3CBR=3E

----8b8ce11283a25abc12c7--

------------------------------

Date: Thu, 1 Apr 2010 08:17:28 +1100
From: Mark Dupal <[hidden email]>
Subject: Dupal, Mark is out of the office.

I will be out of the office starting 01/04/2010 and will not return
until
06/04/2010.

I will have limited email access and will respond to your message when I
return.

P Please consider the environment before printing this email - 3
sheets o=
f A4 paper =3D 1 litre of water This message is intended only for the
addre=
ssee. If you are not the intended recipient you are notified that
disclos=
ing, copying, distributing or taking any action in reliance of the
contents=
of this information is strictly prohibited. =

------------------------------

Date: Wed, 31 Mar 2010 13:46:32 -0800
From: mps23 <[hidden email]>
Subject: Re: Olympus FV1000 vs Nikon A1

Here I have attached the quote - minus our information and the
specific reps (to protect everyone's identity). But, I am very
concerned that others have spent significantly less for similar
systems. Any input you might have would be greatly appreciated.

Thanks,

MP

>Dear M. P. Stein
>
>You did not say how the confocal was configured. Having just
>purchased a FV1000, the price you mentioned must include a lot of
>stuff. Have you gotten comparable quotes for the exact
>same confocal from Leica, Zeiss and Nikon?
>
>Dick
>
>----- Original Message -----
>From: mps23
><<http://n2.nabble.com/user/SendEmail.jtp?type=node&node=4833621&i=0>[h

idden
>email]>
>Date: Wednesday, March 31, 2010 1:03 pm
>Subject: Re: Olympus FV1000 vs Nikon A1
>To:
><http://n2.nabble.com/user/SendEmail.jtp?type=node&node=4833621&i=1>[hi
dden

>email]
>
>
>> Hello all,
>>
>> We are currently going to purchase a new confocal, comparing the
>> OlympusFV1000 spectral, Leica SP5, Nikon A1 and Zeiss 710.
>> As said in other posts,
>> this is not a fair comparison since the FV1000 is approximately
>> 10 years
>> old. If this is the case, is it appropriate that the
>> FV1000 spectral is
>> being sold for $350,000? I understand the Zeiss 710 since
>> it is new
>> technology and the Leica and Nikon as well. Any comments -
>> or has anyone
>> recently purchased a FV1000 spectral? I've asked our Rep
>> for reference list
>> - still waiting?
>>
>> Thanks.
>> --
>> View this message in context: http://n2.nabble.com/Olympus-
>> FV1000-vs-Nikon-A1-tp4567055p4832055.html
>> Sent from the Confocal Microscopy List mailing list archive at
>> Nabble.com.
>>
>> --
>> BEGIN-ANTISPAM-VOTING-LINKS
>> ------------------------------------------------------
>>
>> Teach CanIt if this mail (ID 1019668206) is spam:
>> Spam:
>> https://antispam.osu.edu/b.php?i=1019668206&m=a3c72729dd07&c=sNot
>>spam:
>>https://antispam.osu.edu/b.php?i=1019668206&m=a3c72729dd07&c=n
>> Forget vote:
>> https://antispam.osu.edu/b.php?i=1019668206&m=a3c72729dd07&c=f---
>> ---------------------------------------------------
>> END-ANTISPAM-VOTING-LINKS
>>
>
>Richard W. Burry, Ph.D.
>Department of Neuroscience, College of Medicine
>Campus Microscopy and Imaging Facility, Director
>The Ohio State University
>Associate Editor, Journal of Histochemistry and Cytochemistry
>277 Biomedical Research Tower
>460 West Twelfth Avenue
>Columbus, Ohio 43210
>Voice 614.292.2814 Cell 614.638.3345 Fax 614.247.8849
>
>
>
>View message @
><http://n2.nabble.com/Olympus-FV1000-vs-Nikon-A1-tp4567055p4833621.html
>http://n2.nabble.com/Olympus-FV1000-vs-Nikon-A1-tp4567055p4833621.html
>To unsubscribe from Re: Olympus FV1000 vs Nikon A1,
>< (link removed) ==>click
>here.

--
Mary-Pat Stein
374 W. Mariposa St.
Altadena, CA 91001
cell: 203-376-0312
home: 626-296-3477

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End of CONFOCALMICROSCOPY Digest - <first ever> to 31 Mar 2010 (#2010-1)
************************************************************************