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Hi Darren,
as Johannes has pointed out, three-photon excitation might be a good option, as all those light sources won't help if the UV light doesn't go through your optics. Based on an earlier discussion on this list (many years ago) I've tried visualising protein crystals based on tryptophane excitation, and it worked just fine on a standard Leica SP5 with Mai Tai laser (probably tuned to 840nm, I guess, I can't remember the details). But the sensitivity wasn't great, it worked well with densely packed big crystals, but we couldn't get any fluorescence from a protein solution, even at very high concentrations. Which could be a good or bad thing for you - good if your substance works, because you still might not get much tryptophane background.
Good luck,
Martin
________________________________________
Martin Spitaler, PhD
Head of the
Imaging Facility
Max Planck Institute of Biochemistry
Am Klopferspitz 18
82152 Martinsried
Germany
Tel: +49 (0)89 8578-3971
E-mail:
[hidden email]
Website:
http://www.biochem.mpg.de/en/facilities/imaging
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