Re: getting times from ZEN files - CONFOCALMICROSCOPY Digest - 18 Sep 2012 to 19 Sep 2012 (#2012-224)

[mcammer]

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If you use the LOCI import plugins with ImageJ and click the show metadata button, all the times of image collection are shown in a text window.  You can also go the extra step of reading these into ImageJ and having them printed on each image or stored numerically for analysis.  (Well, if you write a script or macro; it's been on my list of things to do for over a year but haven't gotten to it yet.)

_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

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From: Confocal Microscopy List [[hidden email]] on behalf of Liang, Zhangyi [[hidden email]]
Sent: Thursday, September 20, 2012 4:42 PM
To: [hidden email]
Subject: Re: CONFOCALMICROSCOPY Digest - 18 Sep 2012 to 19 Sep 2012 (#2012-224)

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Hello, All,
I'm asking for help on Zeiss ZEN software:
Can I get the information that what time an image is taken? I took a few files on one sample continually and I need to know the time interval between two images. I cannot find the information that when a frame was recorded by the microscope.
Thank you so much!
Zhangyi


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From: Confocal Microscopy List [[hidden email]] On Behalf Of CONFOCALMICROSCOPY automatic digest system [[hidden email]]
Sent: Thursday, September 20, 2012 1:02 AM
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Subject: CONFOCALMICROSCOPY Digest - 18 Sep 2012 to 19 Sep 2012 (#2012-224)

There are 2 messages totalling 112 lines in this issue.

Topics of the day:

  1. Sealing cover slips (2)

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Date:    Wed, 19 Sep 2012 07:39:19 +0000
From:    "Littlejohn, George" <[hidden email]>
Subject: Re: Sealing cover slips

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Hi Mike,

I like using Carolina Observation Gel (http://www.carolina.com/product/life=
+science/microscope+slides/protist+slides/carolina+observation+gel.do, no c=
ommercial interest).=20

It is mouldable and non-toxic polydimethylsiloxane, which can be made into =
custom gaskets to seal slides (I cut them to shape with a needle). It allow=
s gas exchange, but minimises evaporation.

Best wishes,
George

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Dr. George Littlejohn
School of Biosciences,
University of Exeter,
Halpin Laboratory,
Geoffrey Pope Building,
Stocker Road,
EX4 4QD, UK
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On 18 Sep 2012, at 21:44, "Mike Tighe" <[hidden email]> wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=3Dconfocalmicroscopy
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>=20
> Does anybody know of a non toxic seal for coverslips? I am looking at liv=
e-dead stained bacteria (hoping to see them die) but I think that my agaros=
e is shinking due to evaporation over time. I am worried that nail polish o=
r vacuum grease will affect my results.
>=20
>=20
>=20
> As usual, thanks for any help!
>=20
> Mike

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Date:    Wed, 19 Sep 2012 14:50:48 +0530
From:    ruchi jain <[hidden email]>
Subject: Re: Sealing cover slips

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Hi,
I don't know it can be funny but one scientist at JNU, India uses feviquick
to seal and he has validated that feviquick does not interfere in imaging
or viability.I myself have never done this.

On Wed, Sep 19, 2012 at 2:12 AM, Mike Tighe <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Does anybody know of a non toxic seal for coverslips? I am looking at
> live-dead stained bacteria (hoping to see them die) but I think that my
> agarose is shinking due to evaporation over time. I am worried that nail
> polish or vacuum grease will affect my results.
>
>
>
> As usual, thanks for any help!
>
> Mike
>

--
regards
Ruchi jain,
Graduate student,
Professor Deepak Saini Lab,
Department of MRDG,
IISc,Bangalore,
Email id: [hidden email]
Contact no.080-22932574

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End of CONFOCALMICROSCOPY Digest - 18 Sep 2012 to 19 Sep 2012 (#2012-224)
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