Re: include OTF in OME-TIFF image file

[Daniel James White]

Hi Jason,


On Jul 12, 2010, at 7:02 AM, CONFOCALMICROSCOPY automatic digest system wrote:

>  We did add and keep the objective OTF, since we felt
> that this should be stored with the image (we know of no examples of this
> being used unfortunately).
>
>
> Cheers,
>
> Jason



I wonder if the DeltaVision microscope systems, and even OMX,
that always do know their OTF (so long as you mounted the sample and chose the right immersion oil)
could be persuaded to store the OTF image along with the z stack...

thats one place where this really could/should be used?

At least in an OME database, one might also have the OTFs from the deltavision microscope system,
so that the OTF and image could be linked somehow?

Dan


Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Visualisation, Processing and Analysis
Light Microscopy and Image Processing Facilities
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany

[phone] (German Mobile)
[phone] (Work phone at MPI-CBG)
[phone] (Fax MPI-CBG LMF)

http://www.bioimagexd.net  BioImageXD
http://pacific.mpi-cbg.de                Fiji -  is just ImageJ (Batteries Included)
http://www.chalkie.org.uk                Dan's Homepages
https://ifn.mpi-cbg.de  Dresden Imaging Facility Network
dan (at) chalkie.org.uk
( white (at) mpi-cbg.de )

[Jason Swedlow]

Hi Dan-

Our preference would be to get this happening in a number of file formats.  it will be on the things to add for compliance, certainly.

Thanks for the comments, Dan.

Cheers,

Jason

On Mon, Jul 12, 2010 at 9:41 AM, Daniel James White <[hidden email]> wrote:

Hi Jason,


On Jul 12, 2010, at 7:02 AM, CONFOCALMICROSCOPY automatic digest system wrote:

>  We did add and keep the objective OTF, since we felt
> that this should be stored with the image (we know of no examples of this
> being used unfortunately).
>
>
> Cheers,
>
> Jason



I wonder if the DeltaVision microscope systems, and even OMX,
that always do know their OTF (so long as you mounted the sample and chose the right immersion oil)
could be persuaded to store the OTF image along with the z stack...

thats one place where this really could/should be used?

At least in an OME database, one might also have the OTFs from the deltavision microscope system,
so that the OTF and image could be linked somehow?

Dan


Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Visualisation, Processing and Analysis
Light Microscopy and Image Processing Facilities
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany

[phone] (German Mobile)
[phone] (Work phone at MPI-CBG)
[phone] (Fax MPI-CBG LMF)

http://www.bioimagexd.net       BioImageXD
http://pacific.mpi-cbg.de               Fiji -  is just ImageJ (Batteries Included)
http://www.chalkie.org.uk               Dan's Homepages
https://ifn.mpi-cbg.de                  Dresden Imaging Facility Network
dan (at) chalkie.org.uk
( white (at) mpi-cbg.de )

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