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** Vendor Reply **
Posted on behalf of my esteemed colleague, Nick Dolman, here at Thermo Fisher Scientific:
The following two papers from the Svoboda lab talk a little about this with respect to calcium indicators.
Maravall et al 2000 Biophys J (https://www.ncbi.nlm.nih.gov/pubmed/10777761)
Yasuda et al 2004 Sci STKE (https://www.ncbi.nlm.nih.gov/pubmed/14872098)
They also cite these additional studies
Tsien & Pozzan (https://www.ncbi.nlm.nih.gov/pubmed/2747529)
Harkins et al 1993 (https://www.ncbi.nlm.nih.gov/pubmed/8218910)
O’Malley et al 1999 (https://www.ncbi.nlm.nih.gov/pubmed/10231797)
Busa et al 1992 (https://www.ncbi.nlm.nih.gov/pubmed/1623501)
Cheers,
Nick
Nick J Dolman Ph.D.
Senior Staff Scientist
Research & Development
Thermo Fisher Scientific
100 Technology Drive | Pittsburgh, PA 15219, USA
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From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of MODEL, MICHAEL
Sent: Tuesday, September 27, 2016 5:45 PM
To: [hidden email]
Subject: intracellular ion probes
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Dear All,
Intracellular ion probes behave differently in the cytoplasm than in the buffer - that is well known. I recall that I read somewhere that this difference is because of viscosity or
because of interactions with proteins or maybe because of something else - I got an impression that someone has studied it. But I don't remember where I saw this and cannot think of the right keyword to find the information again. I would be very thankful
if someone points me in the right direction.
Mike Model
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