Re: less than 1x objective

[Dr. Judith Drazba]

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George,

I don't know if this will work on a Zeiss, but on a Leica, you can image at about 0.5X magnification by removing the objective, putting the Bertrand lens in place and using the slider on the Bertrand to focus.

Judy

 Judith Drazba, Ph.D.  Staff and Director
 Imaging and Flow Cytometry Core- LRI
 Cleveland Clinic, NB10  |  9500 Euclid Ave.   |  Cleveland, OH 44195 
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of George McNamara
Sent: Friday, December 02, 2016 4:52 PM
To: [hidden email]
Subject: Re: 1x objective

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Hi Loren,

I do not have access to any high end research microscopes at the moment
- I distinctly remember being able to do this with the Zeiss Axiovert 200's I managed in Miami. However, I now think it was one of these (and don't have a 'vert at home to test this):

* no objective lens

or

* no objective lens + "telescope" position on the eyepiece turret (which would only be useful for eye or some kind of eyepiece path -> camera thingy).

thanks for making me think about this more.

George


On 12/2/2016 3:26 PM, Quintanar, Loren wrote:

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>
> George,
>
> We have an Observer Z1 with a 1x optovar inside. I tried viewing a sample as you suggested and I did not see anything through an empty objective hole...
>
> I suspect that the optovar does not actually form an image? Correct me if I am wrong.
>
> Loren Quintanar
> Research Associate II
> Light Microscopy Core Facility
> Beckman Research Institute
> City of Hope
> x62874
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]] On Behalf Of George McNamara
> Sent: Friday, December 02, 2016 9:47 AM
> To: [hidden email]
> Subject: Re: 1x objective
>
> *****
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>
> Hi Jeremy,
>
> if your microscope has an optovar built in to the stand, use an empty position with the optovar in place.
>
> George
>
>
> On 12/2/2016 5:56 AM, Jeremy Adler wrote:
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>>
>> We want to mount a  cheap 1x objective on a Zeiss microscope, Any
>> suggestions
>>
>> resolution is no very important and we really just want to use the motorized stage to screen a multiwell plate.
>>
>> ====================================
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>> Jeremy Adler PhD, senior research engineer Image Analysis and
>> Microscopy [hidden email]
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--


George McNamara, PhD
Houston, TX 77054
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https://www.linkedin.com/in/georgemcnamara
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http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650

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[Stanislav Vitha-2]

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Yes,
taking out the objective and using a Bertrand lens will work on Zeiss, I just checked. I get a little less than 1x mag this way.
On my old Axiophot, there is some strange vignetting  and the setup is not parfocal - the Bertrand  lens focus mechanism did not have enough range to bring the sample to focus, I had to get the slide about 2 mm closer to the objective turret to get an acceptable image.

If I was doing just brightfield, I would rather put the microwell plate on a flatbed scanner and scan it.
If you need/want to do the imaging on a microscope, I would go with recommendations of previous posters here and get a cheap 1x objective off Ebay. Either infinity or 160mm tube length design should be good enough for screening.

Stan

Stansilav Vitha
Texas A&M University
Microscopy and Imaging Center
 

>George,
>
>I don't know if this will work on a Zeiss, but on a Leica, you can image at about 0.5X magnification by removing the objective, putting the Bertrand lens in place and using the slider on the Bertrand to focus.
>
>Judy
>
> Judith Drazba, Ph.D.��Staff and Director
>�Imaging and Flow Cytometry Core- LRI
> Cleveland Clinic, NB10� | �9500 Euclid Ave. � | �Cleveland, OH 44195�
> [phone]
>
>