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***COMMERCIAL RESPONSE***
Dear Laurent,
X-Cite has a 4 LED high-speed switching system called the X-Cite XLED1. You can configure the unit to modules of your choice (
http://www.ldgi.com/x-cite/led-module-configurator/), and switching is well within your requirements. You can contact me offline for more details at
[hidden email]
Regards,
Kavita
Kavita Aswani, PhD
Senior Applications Scientist - Life Sciences, Lumen Dynamics Group Inc
Direct Tel. 905 812-3342| Cell. 647 290 3506
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Mike Buchin
Sent: October-21-13 11:16 AM
To:
[hidden email]
Subject: Re: modulation (kHz) of a regular light-source for optogenetic-like experiments on a 2P Scope.
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No commercial interest on my part. Prizmatix makes some high power multicolor LED modules with 30 KHz 5 microsecond switching times.
Mike
________________________________________
From: Confocal Microscopy List [
[hidden email]] on behalf of Gelman, Laurent [
[hidden email]]
Sent: Monday, October 21, 2013 6:01 AM
To:
[hidden email]
Subject: modulation (kHz) of a regular light-source for optogenetic-like experiments on a 2P Scope.
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Hi everybody,
A colleague of mine, imaging with a 2-photon microscope calcium signaling in retina upon light stimulation has the following technical problem:
Retina needs to be stimulated with different patterns and colors between two scans. So the pattern needs to be modulated in the 2kHz range, when the scanning mirrors jump into a new row.
The current setup for light pattern stimulation consists of a normal beamer (used normally for PowerPoint presentations) where the light source has been replaced by a LED (Thorlabs) which can be easily modulated.
My colleague would like to be able to apply many more different wavelengths than the 3 available on the Thorlabs device. We will test other LED light sources from the market, but ideally, we would make use of a Polychrome V that is available here and would provide light from 320 to 680nm. Of course, the problem here is the modulation of the light source. We thought of a white laser, but we wish we could stick to cheap solutions and also to setups which could be easily mounted/transferred from one scope to the other one...
Many thanks in advance for your help!
Best regards,
Laurent.
_____________________________________________
Laurent Gelman, PhD
Head of Facility for Advanced Imaging and Microscopy (Light Microscopy)
Friedrich Miescher Institut
WRO 1066.2.16
Maulbeerstrasse 66
CH-4058 Basel
Fix: +41 (0)61 696 35 13
Mobile: +41 (0)79 618 73 69
Fax: +41 (0)61 697 39 76
www.fmi.ch<
http://www.fmi.ch/>
www.microscopynetwork.unibas.ch/<
http://www.microscopynetwork.unibas.ch/>
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