Re: organoid imaging **commercial response**

[Benjamin Schauer]

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Hello Mary Ellen,



If you're looking for labware for your organoid imaging applications, please allow me to recommend looking into the ibidi µ-Slide III 3D Perfusion<https://ibidi.com/channel-slides/53--slide-iii-3d-perfusion.html>. This slide is designed for the cultivation and perfusion of 3D cell structures. If perfusion of the sample is not needed, I recommend looking at the µ-Slide Angiogenesis<https://ibidi.com/chambered-coverslips/41--slide-angiogenesis.html> (both glass and polymer coverslip options available) which is specifically designed for imaging with gels. A sample of both products can be easily requested from the webpages.



Additionally, ibidi has a new Micropatterning technology<https://ibidi.com/content/411-micropatterning-as-a-tool-for-cell-based-assay-imaging> that allows for the immobilization of 3D cell aggregates (e.g. spheroids/organoids) on a slide. Dr. Jan Schwarz, the Head of ibidi R&D, recently gave a talk about this technology at the Aurox Conference on Microscopy (Youtube link to recorded talk<https://www.youtube.com/watch?v=e-Ek8rSRruQ>). Dr. Schwarz will be hosting another webinar on the topic coming up on May 12th<https://ibidi.com/content/405-live-webinar-registration?t=2>, if you’re interested in learning more about the technology.



If you have any questions the products, please do not hesitate to reach out.



Regards,



Ben Schauer

VP Sales



ibidi USA, Inc.

2920 Marketplace Drive

STE 102

Fitchburg, WI 53719

P: 844.276.6363

F: 608.441.8383

[hidden email]



-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Mary Ellen Pease
Sent: Tuesday, April 28, 2020 9:22 AM
To: [hidden email]
Subject: organoid imaging



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Hi all,



I have a user who would like to image live organoids via confocal/multiphoton without jeopardizing the sample. Has anyone figured out a material in which the organoid could be placed that would eliminate movement/drift during imaging, without damaging it so the sample could go back into culture afterwards? So far, the papers I've looked at on this topic cryoembed them at a certain time point for immunohistochemistry. My user's lab has samples which are expressing GFP and RFP, and would like to look at them in vivo. Someone in our department suggested putting them in a mix of matrigel and culture media, which could slow them down temporarily, but in my experience, matrigel attenuates the signal. We have a multiphoton system on an inverted Zeiss platform (LSM 710). we can image using an objective inverter and dipping lenses, or from the bottom through a coverglass dish. Since we are working remotely at this point in time, I was hoping to get some direction from this group.



Best,



Mary Ellen Pease

Manager, Wilmer Microscopy and Imaging Core Facility (MICF) Johns Hopkins SOM, Baltimore MD