Re: recommended distortion target for multiphoton?

[Alex Corbett]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

I have recently joined the confocal microscopy list, so apologies if this post is not reaching the
correct people. I am following up on a thread I was forwarded by a colleague from September
2014 regarding targets for determining distortion over an imaging volume. We have been
trialling a new solution which I have found particularly useful for sensitive measurements of
distortion within an image volume.

The solution is a fluorescent slide in which a 3D fabrication pattern has been laser written. The
fabrication points appear dark in single and two-photon fluorescence. There is the flexibility to
write any pattern although I have so far been using a columnar pattern on a 10um square
grid. The plastic slides are very robust and come in a range of fluorophores. If you think this
approach might be useful, you can find more details by first downloading the (open access)
paper describing it here:
http://journal.frontiersin.org/article/10.3389/fphys.2014.00384/abstract

If you would like to try it for yourself then please contact me and we will see about arranging a
free trial.

Best regards, Alex.

[Kilgore, Jason A.]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi, Alex,

Alex,

How closely can you incise the points?  1um apart?  Less?

Jason



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alex Corbett
Sent: Friday, February 27, 2015 9:05 AM
To: [hidden email]
Subject: Re: recommended distortion target for multiphoton?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

I have recently joined the confocal microscopy list, so apologies if this post is not reaching the correct people. I am following up on a thread I was forwarded by a colleague from September
2014 regarding targets for determining distortion over an imaging volume. We have been trialling a new solution which I have found particularly useful for sensitive measurements of distortion within an image volume.

The solution is a fluorescent slide in which a 3D fabrication pattern has been laser written. The fabrication points appear dark in single and two-photon fluorescence. There is the flexibility to write any pattern although I have so far been using a columnar pattern on a 10um square grid. The plastic slides are very robust and come in a range of fluorophores. If you think this approach might be useful, you can find more details by first downloading the (open access) paper describing it here:
http://journal.frontiersin.org/article/10.3389/fphys.2014.00384/abstract

If you would like to try it for yourself then please contact me and we will see about arranging a free trial.

Best regards, Alex.