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Dear Jeff and Deepak,
You might be interested to look at the lightguide- and prism-based TIRF geometries that provide clean TIRF effect and are easier to obtain uniform intensity of the evanescent wave: TIRF-Labs.com; TIRFmicroscopy.com
Best regards,
Alexander Asanov, Ph.D.
President, TIRF Labs
Cary, NC 27519
TIRF-Labs.com; TIRFmicroscopy.com
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