Re: spinning disc X1 or W1 - COMMERCIAL RESPONSE

[WAINWRIGHT James]

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*** COMMERCIAL RESPONSE ***

Dear Virginie,

I'll address your questions one-by-one:

1) Illumination - the CSU-W is approximately ~1/4 the power density of the CSU-X. (This drops to ~1/8 of the power if you choose to use the smaller 25 micron disk for low power objectives.)

This is primarily due to the area of illumination, but also partly due to the increased pinhole spacing in the CSU-W, which is how it reduces cross-talk to allow imaging of thicker specimens.

Thus, you could consider that you need to use four times the exposure time or four times the laser power. However, in terms of illumination/excitation amount, this would be the same as the CSU-X.


2) No, it's not a problem to image fast processes on the CSU-W, as long as you have the laser power. See item above and #4 (Borealis) below.


3) Comparing EMCCD with sCMOS is best shown here:

https://www.andor.com/learning-academy/a-comparison-of-scmos-and-emccd-digital-camera-technologies-for-spinning-disk-confocal-microscopy-application-note

I would say that the choice comes down to how large a field of view you wish to use.


4) Borealis - this is especially significant with the CSU-W.

On a CSU-X, Borealis will approximately double the excitation power. On a CSU-W, it will approximately triple it! In both cases, the illumination will be considerably more homogeneous too. Thus, a Borealis CSU-W is almost equivalent in terms of power density to the standard CSU-X.


Hope this is of some use!

Best regards,

James

James Wainwright
Product Support Engineer (Microscopy Systems)
Europe, Middle East & Africa

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Mob: [phone]
Fax: [phone]
Web:  www.andor.com/ContactSupport.aspx?type=s



-----Original Message-----


On 3/4/2016 1:39 PM, SUBSCRIBE CONFOCALMICROSCOPY Virginie Lecaudey wrote:

> *****
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> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello,
>
> my lab is planning on purchasing a spinning disc system to perform
> live imaging on zebrafish embryos. We have experience with the Zeiss
> setup (CSU-X1 and Zen Blue
> software) with a
> simple axiocam camera and older experience with different version of
> the PerkinElmer spinning discs with Hamamatso EM-CCD camera.
> Working with zebrafish, we are quite tempting to switch to the CSU-W1
> proposed by Nikon/Andor but would be really happy to get opinion of
> experts on:
>
> 1) How do the CSU-W1 compare to the X1 in terms of required illumintation?
> Do we need
> much longer exposure time (and thus much more light on the sample...)?
>
> 2) Is it really a problem to image fast processes with the W1
>
> 3) in terms of camera, how does the Zyla 4.2 sCMOS compare to the iXon
> EMCCD camera?
>
> 4) Does anybody have experience with the Borealis system from Andor?
> How much does it really improve homogeneity of illumination?
>
> Many thanks in advance for any advice and comments!
>
> Virginie
>

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[Yiwei.Jia]

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Kevin Jia