Re: used microscope for home-building a sted (super-res) system?

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Higdon, Michael Higdon, Michael
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Re: used microscope for home-building a sted (super-res) system?

Re: used microscope for home-building a sted (super-res) system?

Can we sell to Pakistan?

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----- Original Message -----
From: Confocal Microscopy List <[hidden email]>
To: [hidden email] <[hidden email]>
Sent: Mon Mar 09 18:03:38 2009
Subject: used microscope for home-building a sted (super-res) system?

To build such a system most economically, I’d like to get some opinions on component prices.



Primarily, I am looking for a used epifluorescence microscope that can be equipped with a piezo-electric stage. My initial web searches have not yielded much on the used equipment market.



But at the minimum, I’d like to salvage a suitable ‘shell’ from somewhere. The more optics there are on it, the better.



If anyone has some ideas to share regarding the other components needed to build a STED system, please share by all means. For example, is it better to go for a blue diode or a Ti:Sapphire laser?



I haven’t seen a STED system in action. But it could be useful for pathologists in a developing countries where EM’s are very costly to buy and maintain. I used to do 3DEM, but need to adapt myself to the environment here.



Regards,



Usman Qazi, PhD

School of Science and Engineering

LUMS

DHA, Lahore Cantt, Pakistan



sse.lums.edu.pk



This email message, together with any attachments, is for the sole use of the intended recipient(s) and is the confidential information of Applied Precision Inc. If you are not the intended recipient, your review, use, disclosure, copying or dissemination of this email message or its attachments, or the information contained therein, is strictly prohibited. If you are not the intended recipient or if you think this email was sent to you in error, please notify the sender by reply email and delete this message and its attachments, as well as all copies, from your system.


Higdon, Michael Higdon, Michael
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Re: used microscope for home-building a sted (super-res) system?

Re: used microscope for home-building a sted (super-res) system?

List . . . Sorry for the intrusion.  The question was ment for a colleague.
--------------------------
Sent from my BlackBerry Wireless Device

This message was sent via Blackberry.

----- Original Message -----
From: Confocal Microscopy List <[hidden email]>
To: [hidden email] <[hidden email]>
Sent: Mon Mar 09 18:32:53 2009
Subject: Re: used microscope for home-building a sted (super-res) system?

Can we sell to Pakistan?

--------------------------
Sent from my BlackBerry Wireless Device

This message was sent via Blackberry.

----- Original Message -----
From: Confocal Microscopy List <[hidden email]>
To: [hidden email] <[hidden email]>
Sent: Mon Mar 09 18:03:38 2009
Subject: used microscope for home-building a sted (super-res) system?

To build such a system most economically, I’d like to get some opinions on component prices.



Primarily, I am looking for a used epifluorescence microscope that can be equipped with a piezo-electric stage. My initial web searches have not yielded much on the used equipment market.



But at the minimum, I’d like to salvage a suitable ‘shell’ from somewhere. The more optics there are on it, the better.



If anyone has some ideas to share regarding the other components needed to build a STED system, please share by all means. For example, is it better to go for a blue diode or a Ti:Sapphire laser?



I haven’t seen a STED system in action. But it could be useful for pathologists in a developing countries where EM’s are very costly to buy and maintain. I used to do 3DEM, but need to adapt myself to the environment here.



Regards,



Usman Qazi, PhD

School of Science and Engineering

LUMS

DHA, Lahore Cantt, Pakistan



sse.lums.edu.pk









This email message, together with any attachments, is for the sole use of the intended recipient(s) and is the confidential information of Applied Precision Inc. If you are not the intended recipient, your review, use, disclosure, copying or dissemination of this email message or its attachments, or the information contained therein, is strictly prohibited. If you are not the intended recipient or if you think this email was sent to you in error, please notify the sender by reply email and delete this message and its attachments, as well as all copies, from your system.


Franklin, Peter Franklin, Peter
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Re: used microscope for home-building a sted (super-res) system?

In reply to this post by Higdon, Michael
Re: used microscope for home-building a sted (super-res) system?

Good qustion. Ask Bryan

This message was sent via Blackberry.

----- Original Message -----
From: Confocal Microscopy List <[hidden email]>
To: [hidden email] <[hidden email]>
Sent: Mon Mar 09 18:32:53 2009
Subject: Re: used microscope for home-building a sted (super-res) system?

Can we sell to Pakistan?

--------------------------
Sent from my BlackBerry Wireless Device

This message was sent via Blackberry.

----- Original Message -----
From: Confocal Microscopy List <[hidden email]>
To: [hidden email] <[hidden email]>
Sent: Mon Mar 09 18:03:38 2009
Subject: used microscope for home-building a sted (super-res) system?

To build such a system most economically, I’d like to get some opinions on component prices.



Primarily, I am looking for a used epifluorescence microscope that can be equipped with a piezo-electric stage. My initial web searches have not yielded much on the used equipment market.



But at the minimum, I’d like to salvage a suitable ‘shell’ from somewhere. The more optics there are on it, the better.



If anyone has some ideas to share regarding the other components needed to build a STED system, please share by all means. For example, is it better to go for a blue diode or a Ti:Sapphire laser?



I haven’t seen a STED system in action. But it could be useful for pathologists in a developing countries where EM’s are very costly to buy and maintain. I used to do 3DEM, but need to adapt myself to the environment here.



Regards,



Usman Qazi, PhD

School of Science and Engineering

LUMS

DHA, Lahore Cantt, Pakistan



sse.lums.edu.pk









This email message, together with any attachments, is for the sole use of the intended recipient(s) and is the confidential information of Applied Precision Inc. If you are not the intended recipient, your review, use, disclosure, copying or dissemination of this email message or its attachments, or the information contained therein, is strictly prohibited. If you are not the intended recipient or if you think this email was sent to you in error, please notify the sender by reply email and delete this message and its attachments, as well as all copies, from your system.


Jerry Sedgewick-2 Jerry Sedgewick-2
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is confocal listserv working?

This is just a test message to see if the listserv is operating on 16March09


Franklin, Peter wrote:

>
> Good qustion. Ask Bryan
>
> This message was sent via Blackberry.
>
> ----- Original Message -----
> From: Confocal Microscopy List <[hidden email]>
> To: [hidden email] <[hidden email]>
> Sent: Mon Mar 09 18:32:53 2009
> Subject: Re: used microscope for home-building a sted (super-res) system?
>
> Can we sell to Pakistan?
>
> --------------------------
> Sent from my BlackBerry Wireless Device
>
> This message was sent via Blackberry.
>
> ----- Original Message -----
> From: Confocal Microscopy List <[hidden email]>
> To: [hidden email] <[hidden email]>
> Sent: Mon Mar 09 18:03:38 2009
> Subject: used microscope for home-building a sted (super-res) system?
>
> To build such a system most economically, I’d like to get some
> opinions on component prices.
>
>
>
> Primarily, I am looking for a used epifluorescence microscope that can
> be equipped with a piezo-electric stage. My initial web searches have
> not yielded much on the used equipment market.
>
>
>
> But at the minimum, I’d like to salvage a suitable ‘shell’ from
> somewhere. The more optics there are on it, the better.
>
>
>
> If anyone has some ideas to share regarding the other components
> needed to build a STED system, please share by all means. For example,
> is it better to go for a blue diode or a Ti:Sapphire laser?
>
>
>
> I haven’t seen a STED system in action. But it could be useful for
> pathologists in a developing countries where EM’s are very costly to
> buy and maintain. I used to do 3DEM, but need to adapt myself to the
> environment here.
>
>
>
> Regards,
>
>
>
> Usman Qazi, PhD
>
> School of Science and Engineering
>
> LUMS
>
> DHA, Lahore Cantt, Pakistan
>
>
>
> sse.lums.edu.pk
>
>
>
>
>
>
>
>
>
> *This email message, together with any attachments, is for the sole
> use of the intended recipient(s) and is the confidential information
> of Applied Precision Inc. If you are not the intended recipient, your
> review, use, disclosure, copying or dissemination of this email
> message or its attachments, or the information contained therein, is
> strictly prohibited. If you are not the intended recipient or if you
> think this email was sent to you in error, please notify the sender by
> reply email and delete this message and its attachments, as well as
> all copies, from your system.*
>
>


--
Jerry (Gerald) Sedgewick
Program Director, Biomedical Image Processing Lab (BIPL)
Department of Neuroscience, University of Minnesota
312 Church St. SE, 1-205 Hasselmo Hall
Minneapolis, MN  55455
(612) 624-6607
[hidden email]
http://www.bipl.umn.edu
Author: "Scientific Imaging with Photoshop: Methods, Measurement and Output."

Rawlight.com (dba Sedgewick Initiatives)
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