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Recommendations for commercial multi-photon system purchase

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Cameron, Lisa

Recommendations for commercial multi-photon system purchase

I have been investigating commercial multi-photon systems for awhile in order to purchase a system for my Institute's core microscopy facility. Our interest is to have the capability to do intravital imaging on an upright stand, but also be able to have facility users be able to put slides on and use the visible scanner and detectors. I realize this is a tall order for such versatility in one system, but since it is for a core (which needs to bring in revenue), I'm looking for the most flexible system. Does anyone have any suggestions about the most recent systems on the market? Or could you point out factors you think are the most important for making the decision on which company to go with? Please feel free to contact me off line.

I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus MPE and Prairie's system.

(BTW - my own experience is with widefield and confocal live-cell imaging, so I have not done 2-p myself, but have been learning about it for about a year)

Thanks!
- Lisa

---------------------------------------
Lisa Cameron, Ph.D.
Director of Confocal and Light Microscopy
Dana Farber Cancer Institute
44 Binney St.; JF 215
Boston, MA 02115
Office phone: 617-582-8824
Fax: 617-582-8750
[hidden email]


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.

Eric Scarfone

Re: Recommendations for commercial multi-photon system purchase

Hi Lisa
Lucky you to purchase such a machine!!

The beauty with multiphoton excitation is that optical sectioning is
achieved by the limited volume within which photon density is sufficient
for 2 (or more) quasi simultaneous photon absorption events to take
place. For a given laser setting, the thickness within which this occurs
is only determined by the NA of your objective. ALL the fluorescence
coming from your sample is emitted by this NA determined plane. There is
no "out of focus" fluorescence. This is very different from Confocal.

To make full use of this one would like to collect as much of the
fluorescent photons emitted by the samples as possible. The most
effective way to do that is to use non-descanned external detectors both
in the reflected pathway and in the transmitted pathway.

To my knowledge this was implemented first by Warren Zipfel in Watt Webb
lab at Cornell and then commercialized by Bio-Rad on the last system
they put on the market before diseappearing at the end of the previous
century.... After a pretty long absorption/digestion process this was
implemented rather recently by Zeiss.
Others might have come up with similar solution but I do not know about
it.

I'd were you, for this level of price, I'd ask for an on site test with
your own samples. Do not rely on "in factory" demos!!

Cheers
Eric

Eric Scarfone, PhD, CNRS,
Center for Hearing and communication Research
Department of Clinical Neuroscience
Karolinska Institutet

Postal Address:
CFH, M1:02
Karolinska Hospital,
SE-171 76 Stockholm, Sweden

Work: +46 (0)8-517 79343,
Cell: +46 (0)70 888 2352
Fax: +46 (0)8-301876

email: [hidden email]
http://www.ki.se/cfh/

----- Original Message -----
From: "Cameron, Lisa" <[hidden email]>
Date: Wednesday, May 13, 2009 4:14 pm
Subject: Recommendations for commercial multi-photon system purchase
To: [hidden email]

> I have been investigating commercial multi-photon systems for
> awhile in order to
> purchase a system for my Institute's core microscopy facility. Our
> interest is
> to have the capability to do intravital imaging on an upright
> stand, but also be
> able to have facility users be able to put slides on and use the
> visible scanner
> and detectors. I realize this is a tall order for such versatility
> in one
> system, but since it is for a core (which needs to bring in
> revenue), I'm
> looking for the most flexible system. Does anyone have any
> suggestions about the
> most recent systems on the market? Or could you point out factors
> you think are
> the most important for making the decision on which company to go
> with? Please
> feel free to contact me off line.
>
> I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus MPE
> and Prairie's
> system.
>
> (BTW - my own experience is with widefield and confocal live-cell
> imaging, so I
> have not done 2-p myself, but have been learning about it for about
> a year)
>
> Thanks!
> - Lisa
>
> ---------------------------------------
> Lisa Cameron, Ph.D.
> Director of Confocal and Light Microscopy
> Dana Farber Cancer Institute
> 44 Binney St.; JF 215
> Boston, MA 02115
> Office phone: 617-582-8824
> Fax: 617-582-8750
> [hidden email]
>
>
>
> The information in this e-mail is intended only for the person to
> whom it is
> addressed. If you believe this e-mail was sent to you in error and
> the e-mail
> contains patient information, please contact the Partners
> Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to
> you in error
> but does not contain patient information, please contact the sender
> and properly
> dispose of the e-mail.
>

Adrian Smith-6

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Cameron, Lisa

Hi Lisa,

We've been very happy with our (multi-photon only) upright intravital
system from LaVision Biotec and are looking to finalise the purchase of a
second (inverted) system from them in the very near future.

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

> I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus MPE and
> Prairie's
> system.
>
> (BTW - my own experience is with widefield and confocal live-cell imaging,
> so I
> have not done 2-p myself, but have been learning about it for about a
> year)
>
> Thanks!
> - Lisa
>
> ---------------------------------------
> Lisa Cameron, Ph.D.
> Director of Confocal and Light Microscopy
> Dana Farber Cancer Institute
> 44 Binney St.; JF 215
> Boston, MA 02115
> Office phone: 617-582-8824
> Fax: 617-582-8750
> [hidden email]
>
>
>
> The information in this e-mail is intended only for the person to whom it
> is
> addressed. If you believe this e-mail was sent to you in error and the
> e-mail
> contains patient information, please contact the Partners Compliance
> HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in
> error
> but does not contain patient information, please contact the sender and
> properly
> dispose of the e-mail.
>

Armstrong, Brian

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Cameron, Lisa

Recommendations for commercial multi-photon system purchase

Hi Lisa, I think you have seen the best instruments. Why would you look any further? What can you tell US?

I would choose between 710, SP5, and the Prairie Ultima. What you choose depends of course on how you want to use the system.

We have a Zeiss 510NLO system and a Prairie.

I really like the Prairie system for 2P in-vivo work!

I am happy to talk with you at length about the systems if you like. I received much help from those in the microscopy community prior to making my decision.

Cheers,

Brian D Armstrong PhD

Light Microscopy Core Manager

Beckman Research Institute

City of Hope

Dept of Neuroscience

1450 E Duarte Rd

Duarte, CA 91010

626-256-4673 x62872

http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cameron, Lisa
Sent: Wednesday, May 13, 2009 7:05 AM
To: [hidden email]
Subject: Recommendations for commercial multi-photon system purchase

I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus MPE and Prairie's system.

(BTW - my own experience is with widefield and confocal live-cell imaging, so I have not done 2-p myself, but have been learning about it for about a year)

Thanks!
- Lisa

The information in this e-mail is intended only for the person to whom it is

addressed. If you believe this e-mail was sent to you in error and the e-mail

contains patient information, please contact the Partners Compliance HelpLine at

http://www.partners.org/complianceline . If the e-mail was sent to you in error

but does not contain patient information, please contact the sender and properly

dispose of the e-mail.

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Alison J. North

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Cameron, Lisa

Hi Lisa,

This is certainly a very difficult decision to make. We had to go
through the same process in December so I feel your pain! We bought the
Olympus system in the end and are extremely happy with it - it provides
all of the flexibility you need, with multiphoton and visible lasers,
and the ability to switch between the stand being optimized for live
animal work and for imaging slides. That being said, we also very much
liked the LaVision, the Prairie and also the Zeiss 710 - which showed a
huge improvement over our old 510. If you were only interested in
multiphoton imaging, the LaVision has certain advantages, but if you
need visible lasers too then I'm afraid it won't suit your purposes.
The Prairie is also a wonderful system, and we were even favourably
impressed by the software, which I had been led to believe might be less
suitable for a core facility.

I realize I'm not helping at all here - yes, you need to test the
systems for yourself (which you have clearly already done!), but in the
end you may also have to consider factors such as price and local
service in order to make your decision, because there are several
excellent multiphoton systems out there now.

Please feel free to contact me offline if you want to discuss our
decision process in more detail....
Best,
Alison

Cameron, Lisa wrote:

>
> I have been investigating commercial multi-photon systems for awhile
> in order to purchase a system for my Institute's core microscopy
> facility. Our interest is to have the capability to do intravital
> imaging on an upright stand, but also be able to have facility users
> be able to put slides on and use the visible scanner and detectors. I
> realize this is a tall order for such versatility in one system, but
> since it is for a core (which needs to bring in revenue), I'm looking
> for the most flexible system. Does anyone have any suggestions about
> the most recent systems on the market? Or could you point out factors
> you think are the most important for making the decision on which
> company to go with? Please feel free to contact me off line.
>
> I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus MPE and
> Prairie's system.
>
> (BTW - my own experience is with widefield and confocal live-cell
> imaging, so I have not done 2-p myself, but have been learning about
> it for about a year)
>
> Thanks!
> - Lisa
>
> ---------------------------------------
> Lisa Cameron, Ph.D.
> Director of Confocal and Light Microscopy
> Dana Farber Cancer Institute
> 44 Binney St.; JF 215
> Boston, MA 02115
> Office phone: 617-582-8824
> Fax: 617-582-8750
> [hidden email]
>
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and properly
> dispose of the e-mail.
>

--
Alison J. North, Ph.D.,
Research Assistant Professor and
Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office ++ 212 327 7488
Tel: lab ++ 212 327 7486
Fax: ++ 212 327 7489

Cameron Nowell

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Cameron, Lisa

Hi Lisa,

Thought i woudl chip in with my experience as well. We have 2 Olympus MPE Systems, one inverted and one upright that share a common Mai Tai Laser (the laser can only be sent to one scope at a time). The upright is used routinely for intravital imaging and has performed flawlessly. We have imaged zebra fish and mice (both live and dead) on it and acheived some spectacular data sets.

The current configuration has on large 0.95NA water imersion 20x objective on it, but it is easy enough to slip another turret of objectives onto it if required. Not sure if it is true for other system but the olympus system is tuned to work with the 20x objective in multiphoton mode, so putting other objectives on may not result in optimal imaging. For single photon excitation it doesn't matter.

The software is stable and easy enough to use. Users are usually up and going independently after a coupel of hours of training and practice.

The only issue i have with it is that you can not communicate directly with the laser via the Olympus software. You can control the laser power via an ND filter system but other functions (such as wave length tuning) are carried out using a seperate software package installed on a laptop plugged into the laser.

One last thing worth mentioning is that both microscoes are fitted with black acrylic incubators (thanks to Steve Cody for this). They work so well at blocking out light that you can perform MP imaging with the room lights on!

Also look into what the service provided by the companies are in your area. Here in Melbourne Olympus are probaly one of the best for response and quality of service. There is no point getting the best microsocpe out there if the peopel in your area are not that good at keeping it going.

Cheers

Cam

Cameron J. Nowell
Microscpy Manager
Central Resource for Advanced Microscopy
Ludwig Insttue for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA

Office: +61 3 9341 3155
Mobile: +61422882700
Fax: +61 3 9341 3104

http://www.ludwig.edu.au/branch/research/platform/microscopy.htm

________________________________

From: Confocal Microscopy List on behalf of Cameron, Lisa
Sent: Thu 14/05/2009 12:04 AM
To: [hidden email]
Subject: Recommendations for commercial multi-photon system purchase

I have been investigating commercial multi-photon systems for awhile in order to purchase a system for my Institute's core microscopy facility. Our interest is to have the capability to do intravital imaging on an upright stand, but also be able to have facility users be able to put slides on and use the visible scanner and detectors. I realize this is a tall order for such versatility in one system, but since it is for a core (which needs to bring in revenue), I'm looking for the most flexible system. Does anyone have any suggestions about the most recent systems on the market? Or could you point out factors you think are the most important for making the decision on which company to go with? Please feel free to contact me off line.

I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus MPE and Prairie's system.

(BTW - my own experience is with widefield and confocal live-cell imaging, so I have not done 2-p myself, but have been learning about it for about a year)

Thanks!
- Lisa

---------------------------------------
Lisa Cameron, Ph.D.
Director of Confocal and Light Microscopy
Dana Farber Cancer Institute
44 Binney St.; JF 215
Boston, MA 02115
Office phone: 617-582-8824
Fax: 617-582-8750
[hidden email]

The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.

Adrian Smith-6

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Alison J. North

On 14/05/2009, at 1:57 AM, Alison North wrote:

> If you were only interested in multiphoton imaging, the LaVision has
> certain advantages, but if you need visible lasers too then I'm
> afraid it won't suit your purposes.

I'm not sure of the details but the LaVision BioTec did mention some
confocal options to me in passing when we were discussing
specifications recently. We were not interested so I didn't ask any
details but it might be worth clarifying with them what they can
currently offer.

From recollection I think the Prairie was also MP-only, at least when
we were looking (that was one of its selling points). Maybe that has
changed or I'm misrembering?

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

Guy Cox

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Eric Scarfone

Just to keep the record straight, our Leica MP system has non-descanned
detectors in both transmission and epi directions - this was delivered
in 2000, ie 9 years ago. We were a fairly early customer for these. It
was (IMHO) a better implementation than the Bio-Rad one (less sensitive
to room light). Zeiss back then did have non-descanned detection but
only in transmitted direction - it worked quite well so long as your
sample wasn't impossibly thick.

Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861 http://www.guycox.net
______________________________________________

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Eric Scarfone
Sent: Thursday, 14 May 2009 12:46 AM
To: [hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase

Hi Lisa
Lucky you to purchase such a machine!!

The beauty with multiphoton excitation is that optical sectioning is
achieved by the limited volume within which photon density is sufficient

for 2 (or more) quasi simultaneous photon absorption events to take
place. For a given laser setting, the thickness within which this occurs

is only determined by the NA of your objective. ALL the fluorescence
coming from your sample is emitted by this NA determined plane. There is

no "out of focus" fluorescence. This is very different from Confocal.

To make full use of this one would like to collect as much of the
fluorescent photons emitted by the samples as possible. The most
effective way to do that is to use non-descanned external detectors both

in the reflected pathway and in the transmitted pathway.

To my knowledge this was implemented first by Warren Zipfel in Watt Webb

lab at Cornell and then commercialized by Bio-Rad on the last system
they put on the market before diseappearing at the end of the previous
century.... After a pretty long absorption/digestion process this was
implemented rather recently by Zeiss.
Others might have come up with similar solution but I do not know about
it.

I'd were you, for this level of price, I'd ask for an on site test with
your own samples. Do not rely on "in factory" demos!!

Cheers
Eric

Eric Scarfone, PhD, CNRS,
Center for Hearing and communication Research
Department of Clinical Neuroscience
Karolinska Institutet

Postal Address:
CFH, M1:02
Karolinska Hospital,
SE-171 76 Stockholm, Sweden

Work: +46 (0)8-517 79343,
Cell: +46 (0)70 888 2352
Fax: +46 (0)8-301876

email: [hidden email]
http://www.ki.se/cfh/

----- Original Message -----
From: "Cameron, Lisa" <[hidden email]>
Date: Wednesday, May 13, 2009 4:14 pm
Subject: Recommendations for commercial multi-photon system purchase
To: [hidden email]

Internal Virus Database is out-of-date.
Checked by AVG.
Version: 7.5.557 / Virus Database: 270.12.11/2089 - Release Date:
30/04/2009 5:53 PM

Sylvie Le Guyader-2

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Cameron Nowell

I think Olympus has now implemented the tuning of the laser and the
compensation within their software.

Cameron, where did you get these black incubators? I am interested in
getting one done as well.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14157 Huddinge
Sweden
+46 (0)8 608 9240

> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]] On Behalf Of Cameron
> Nowell
> Sent: 14 May 2009 00:28
> To: [hidden email]
> Subject: Re: Recommendations for commercial multi-photon system purchase
>
> Hi Lisa,
>
> Thought i woudl chip in with my experience as well. We have 2 Olympus MPE
> Systems, one inverted and one upright that share a common Mai Tai Laser

(the
> laser can only be sent to one scope at a time). The upright is used
routinely for
> intravital imaging and has performed flawlessly. We have imaged zebra fish
and
> mice (both live and dead) on it and acheived some spectacular data sets.
>
> The current configuration has on large 0.95NA water imersion 20x objective
on it,
> but it is easy enough to slip another turret of objectives onto it if
required. Not sure
> if it is true for other system but the olympus system is tuned to work
with the 20x
> objective in multiphoton mode, so putting other objectives on may not
result in
> optimal imaging. For single photon excitation it doesn't matter.
>
> The software is stable and easy enough to use. Users are usually up and
going
> independently after a coupel of hours of training and practice.
>
> The only issue i have with it is that you can not communicate directly
with the laser
> via the Olympus software. You can control the laser power via an ND filter
system
> but other functions (such as wave length tuning) are carried out using a
seperate
> software package installed on a laptop plugged into the laser.
>
> One last thing worth mentioning is that both microscoes are fitted with
black acrylic
> incubators (thanks to Steve Cody for this). They work so well at blocking
out light
> that you can perform MP imaging with the room lights on!
>
> Also look into what the service provided by the companies are in your
area. Here in
> Melbourne Olympus are probaly one of the best for response and quality of
service.
> There is no point getting the best microsocpe out there if the peopel in
your area

> are not that good at keeping it going.
>
>
> Cheers
>
>
> Cam
>
>
>
> Cameron J. Nowell
> Microscpy Manager
> Central Resource for Advanced Microscopy
> Ludwig Insttue for Cancer Research
> PO Box 2008
> Royal Melbourne Hospital
> Victoria, 3050
> AUSTRALIA
>
> Office: +61 3 9341 3155
> Mobile: +61422882700
> Fax: +61 3 9341 3104
>
> http://www.ludwig.edu.au/branch/research/platform/microscopy.htm
>
>
> ________________________________
>
> From: Confocal Microscopy List on behalf of Cameron, Lisa
> Sent: Thu 14/05/2009 12:04 AM
> To: [hidden email]
> Subject: Recommendations for commercial multi-photon system purchase
>
>
>
> I have been investigating commercial multi-photon systems for awhile in

order to
> purchase a system for my Institute's core microscopy facility. Our
interest is to have
> the capability to do intravital imaging on an upright stand, but also be
able to have
> facility users be able to put slides on and use the visible scanner and
detectors. I
> realize this is a tall order for such versatility in one system, but since
it is for a core
> (which needs to bring in revenue), I'm looking for the most flexible
system. Does
> anyone have any suggestions about the most recent systems on the market?
Or
> could you point out factors you think are the most important for making
the decision
> on which company to go with? Please feel free to contact me off line.
>
> I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus MPE and
> Prairie's system.
>
> (BTW - my own experience is with widefield and confocal live-cell imaging,
so I
> have not done 2-p myself, but have been learning about it for about a
year)

>
> Thanks!
> - Lisa
>
> ---------------------------------------
> Lisa Cameron, Ph.D.
> Director of Confocal and Light Microscopy
> Dana Farber Cancer Institute
> 44 Binney St.; JF 215
> Boston, MA 02115
> Office phone: 617-582-8824
> Fax: 617-582-8750
> [hidden email]
>
> The information in this e-mail is intended only for the person to whom it

is
> addressed. If you believe this e-mail was sent to you in error and the
e-mail
> contains patient information, please contact the Partners Compliance
HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in
error
> but does not contain patient information, please contact the sender and
properly
> dispose of the e-mail.

Cameron Nowell

Re: Recommendations for commercial multi-photon system purchase

Hi Sylvie

The incubators were made in Melbourne by Clear State Solutions (www.clearstatesolutions.com)

Pecon also make them for Leica microscopes (http://www.pecon.biz/?page_id=113)

Cheers

Cam

________________________________

From: Confocal Microscopy List on behalf of Sylvie Le Guyader
Sent: Thu 14/05/2009 6:22 PM
To: [hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase

I think Olympus has now implemented the tuning of the laser and the
compensation within their software.

Cameron, where did you get these black incubators? I am interested in
getting one done as well.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14157 Huddinge
Sweden
+46 (0)8 608 9240

Watkins, Simon C

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Adrian Smith-6

If and when you try the olympus system make sure they bring you the super expensive dipping/coverslip water optic (25X1.12 NA) which they designed specifically for 2p. It is absolutely spectacular, and pretty much the only lens we use for all our mpe work nowadays. When you compare systems it may make you lean towards this particular manufacturer. Of course the LaVision and Prairie scan heads will fit on the Oly stand, not sure whether they will work with this lens but it would be great to find out (anyone out there tried this combo yet).
S.
Simon C. Watkins Ph.D, FRC Path
Professor and Vice Chair Cell Biology and Physiology
Professor Immunology
Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St
Pittsburgh PA 15261
412-352-2277
www.cbi.pitt.edu

________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Adrian Smith [[hidden email]]
Sent: Wednesday, May 13, 2009 8:04 PM
To: [hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase

On 14/05/2009, at 1:57 AM, Alison North wrote:

> If you were only interested in multiphoton imaging, the LaVision has
> certain advantages, but if you need visible lasers too then I'm
> afraid it won't suit your purposes.

I'm not sure of the details but the LaVision BioTec did mention some
confocal options to me in passing when we were discussing
specifications recently. We were not interested so I didn't ask any
details but it might be worth clarifying with them what they can
currently offer.

From recollection I think the Prairie was also MP-only, at least when
we were looking (that was one of its selling points). Maybe that has
changed or I'm misrembering?

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

Adrian Smith-6

Re: Recommendations for commercial multi-photon system purchase

I don't believe there is any technical reason why the 25x Olympus will
not work on the LaVision BioTec or Prairie systems.

However, my understanding is that Olympus will only sell that
objective with a new Olympus MPE system... (which makes it a lot more
than just "super expensive" :).

Extremely disappointing decision on Olympus' part.

I would absolutely love to try the objective on our Olympus microscope
(supplied by LaVision BioTec) but I am unable to. If it performs as
well as the marketing material suggests then I would be willing to pay
a premium for it.

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

On 14/05/2009, at 9:23 PM, Watkins, Simon C wrote:

> If and when you try the olympus system make sure they bring you the
> super expensive dipping/coverslip water optic (25X1.12 NA) which
> they designed specifically for 2p. It is absolutely spectacular,
> and pretty much the only lens we use for all our mpe work nowadays.
> When you compare systems it may make you lean towards this
> particular manufacturer. Of course the LaVision and Prairie scan
> heads will fit on the Oly stand, not sure whether they will work
> with this lens but it would be great to find out (anyone out there
> tried this combo yet).
> S.
> Simon C. Watkins Ph.D, FRC Path
> Professor and Vice Chair Cell Biology and Physiology
> Professor Immunology
> Director Center for Biologic Imaging
> BSTS 225
> University of Pittsburgh
> 3500 Terrace St
> Pittsburgh PA 15261
> 412-352-2277
> www.cbi.pitt.edu
>
> ________________________________________
> From: Confocal Microscopy List [[hidden email]] On
> Behalf Of Adrian Smith [[hidden email]]
> Sent: Wednesday, May 13, 2009 8:04 PM
> To: [hidden email]
> Subject: Re: Recommendations for commercial multi-photon system
> purchase
>
> On 14/05/2009, at 1:57 AM, Alison North wrote:
>
>> If you were only interested in multiphoton imaging, the LaVision has
>> certain advantages, but if you need visible lasers too then I'm
>> afraid it won't suit your purposes.
>
> I'm not sure of the details but the LaVision BioTec did mention some
> confocal options to me in passing when we were discussing
> specifications recently. We were not interested so I didn't ask any
> details but it might be worth clarifying with them what they can
> currently offer.
>
> From recollection I think the Prairie was also MP-only, at least when
> we were looking (that was one of its selling points). Maybe that has
> changed or I'm misrembering?
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia

RICHARD BURRY

Re: Recommendations for commercial multi-photon system purchase

The Olympus 25x 1.05 N.A. objective has an enlarged aperture in the back image plane to allow more light throughput. This requires additional lenses capable of filling the aperture, which Olympus has. While the lens may fit on other microscopes, with ut the filling of this aperture, it will not perform as well as it could.

Dick

----- Original Message -----
From: Adrian Smith <[hidden email]>
Date: Thursday, May 14, 2009 8:02 am
Subject: Re: Recommendations for commercial multi-photon system purchase
To: [hidden email]

> I don't believe there is any technical reason why the 25x
> Olympus will
> not work on the LaVision BioTec or Prairie systems.
>
> However, my understanding is that Olympus will only sell
> that
> objective with a new Olympus MPE system... (which makes it a lot
> more
> than just "super expensive" :).
>
> Extremely disappointing decision on Olympus' part.
>
> I would absolutely love to try the objective on our Olympus
> microscope
> (supplied by LaVision BioTec) but I am unable to. If it performs
> as
> well as the marketing material suggests then I would be willing
> to pay
> a premium for it.
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
>
> On 14/05/2009, at 9:23 PM, Watkins, Simon C wrote:
>
> > If and when you try the olympus system make sure they bring
> you the
> > super expensive dipping/coverslip water optic (25X1.12 NA)
> which
> > they designed specifically for 2p. It is absolutely
> spectacular,
> > and pretty much the only lens we use for all our mpe work
> nowadays.
> > When you compare systems it may make you lean towards
> this
> > particular manufacturer. Of course the LaVision and
> Prairie scan
> > heads will fit on the Oly stand, not sure whether they will
> work
> > with this lens but it would be great to find out (anyone out
> there
> > tried this combo yet).
> > S.
> > Simon C. Watkins Ph.D, FRC Path
> > Professor and Vice Chair Cell Biology and Physiology
> > Professor Immunology
> > Director Center for Biologic Imaging
> > BSTS 225
> > University of Pittsburgh
> > 3500 Terrace St
> > Pittsburgh PA 15261
> > 412-352-2277
> > www.cbi.pitt.edu
> >
> > ________________________________________
> > From: Confocal Microscopy List
> [[hidden email]] On
> > Behalf Of Adrian Smith [[hidden email]]
> > Sent: Wednesday, May 13, 2009 8:04 PM
> > To: [hidden email]
> > Subject: Re: Recommendations for commercial multi-photon
> system
> > purchase
> >
> > On 14/05/2009, at 1:57 AM, Alison North wrote:
> >
> >> If you were only interested in multiphoton imaging, the
> LaVision has
> >> certain advantages, but if you need visible lasers too then I'm
> >> afraid it won't suit your purposes.
> >
> > I'm not sure of the details but the LaVision BioTec did
> mention some
> > confocal options to me in passing when we were discussing
> > specifications recently. We were not interested so I didn't
> ask any
> > details but it might be worth clarifying with them what they can
> > currently offer.
> >
> > From recollection I think the Prairie was also MP-only, at
> least when
> > we were looking (that was one of its selling points). Maybe
> that has
> > changed or I'm misrembering?
> >
> > Regards,
> >
> > Adrian Smith
> > Centenary Institute, Sydney, Australia
>
>
> --
> BEGIN-ANTISPAM-VOTING-LINKS
> ------------------------------------------------------
>
> Teach CanIt if this mail (ID 868940829) is spam:
> Spam:
> https://antispam.osu.edu/b.php?c=s&i=868940829&m=73ec3abfd3abNot
> spam:    https://antispam.osu.edu/b.php?c=n&i=868940829&m=73ec3abfd3ab
> Forget vote:
> https://antispam.osu.edu/b.php?c=f&i=868940829&m=73ec3abfd3ab----
> --------------------------------------------------
> END-ANTISPAM-VOTING-LINKS
>

Eric Scarfone

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Guy Cox

Thanks for the precision Guy. You must be at least as old as I am!
2000 was about the time I installed the Biorad 1024 MP back in France. Did your Leica system actually worked back then (I mean with pico second laser, OS 9 and all?) ;=)

Eric Scarfone, PhD, CNRS,
Center for Hearing and communication Research
Department of Clinical Neuroscience
Karolinska Institutet

Postal Address:
CFH, M1:02
Karolinska Hospital,
SE-171 76 Stockholm, Sweden

Work: +46 (0)8-517 79343,
Cell: +46 (0)70 888 2352
Fax: +46 (0)8-301876

email: [hidden email]
http://www.ki.se/cfh/

----- Original Message -----
From: Guy Cox <[hidden email]>
Date: Thursday, May 14, 2009 3:15 am
Subject: Re: Recommendations for commercial multi-photon system purchase
To: [hidden email]

> Just to keep the record straight, our Leica MP system has non-

> descanneddetectors in both transmission and epi directions - this
> was delivered
> in 2000, ie 9 years ago. We were a fairly early customer for
> these. It
> was (IMHO) a better implementation than the Bio-Rad one (less
> sensitiveto room light). Zeiss back then did have non-descanned
> detection but
> only in transmitted direction - it worked quite well so long as your
> sample wasn't impossibly thick.
>
> Guy
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006

> ______________________________________________

> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861 http://www.guycox.net
> ______________________________________________
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]]On Behalf Of Eric Scarfone
> Sent: Thursday, 14 May 2009 12:46 AM
> To: [hidden email]
> Subject: Re: Recommendations for commercial multi-photon system
> purchase
> Hi Lisa
> Lucky you to purchase such a machine!!
>
> The beauty with multiphoton excitation is that optical sectioning
> is
> achieved by the limited volume within which photon density is
> sufficient
> for 2 (or more) quasi simultaneous photon absorption events to
> take
> place. For a given laser setting, the thickness within which this

> occurs

> is only determined by the NA of your objective. ALL the
> fluorescence
> coming from your sample is emitted by this NA determined plane.
> There is
>
> no "out of focus" fluorescence. This is very different from Confocal.
>
> To make full use of this one would like to collect as much of the
> fluorescent photons emitted by the samples as possible. The most
> effective way to do that is to use non-descanned external
> detectors both
>
> in the reflected pathway and in the transmitted pathway.
>
> To my knowledge this was implemented first by Warren Zipfel in
> Watt Webb
>
> lab at Cornell and then commercialized by Bio-Rad on the last
> system
> they put on the market before diseappearing at the end of the
> previous
> century.... After a pretty long absorption/digestion process this
> was

> implemented rather recently by Zeiss.

> Others might have come up with similar solution but I do not know
> about
> it.
>
> I'd were you, for this level of price, I'd ask for an on site test
> with
> your own samples. Do not rely on "in factory" demos!!
>
> Cheers
> Eric
>
>
> Eric Scarfone, PhD, CNRS,
> Center for Hearing and communication Research
> Department of Clinical Neuroscience
> Karolinska Institutet
>
> Postal Address:
> CFH, M1:02
> Karolinska Hospital,
> SE-171 76 Stockholm, Sweden
>
> Work: +46 (0)8-517 79343,
> Cell: +46 (0)70 888 2352
> Fax: +46 (0)8-301876
>
> email: [hidden email]
> http://www.ki.se/cfh/
>
>
> ----- Original Message -----
> From: "Cameron, Lisa" <[hidden email]>

> Date: Wednesday, May 13, 2009 4:14 pm

> Subject: Recommendations for commercial multi-photon system purchase
> To: [hidden email]
>
> > I have been investigating commercial multi-photon systems for
> > awhile in order to
> > purchase a system for my Institute's core microscopy facility.
> Our
> > interest is
> > to have the capability to do intravital imaging on an upright
> > stand, but also be
> > able to have facility users be able to put slides on and use the
> > visible scanner
> > and detectors. I realize this is a tall order for such
> versatility
> > in one
> > system, but since it is for a core (which needs to bring in
> > revenue), I'm
> > looking for the most flexible system. Does anyone have any
> > suggestions about the

> > most recent systems on the market? Or could you point out

> factors
> > you think are
> > the most important for making the decision on which company to
> go
> > with? Please
> > feel free to contact me off line.
> >
> > I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus
> MPE
> > and Prairie's
> > system.
> >
> > (BTW - my own experience is with widefield and confocal live-
> cell
> > imaging, so I
> > have not done 2-p myself, but have been learning about it for
> about
> > a year)
> >
> > Thanks!
> > - Lisa
> >
> > ---------------------------------------
> > Lisa Cameron, Ph.D.
> > Director of Confocal and Light Microscopy
> > Dana Farber Cancer Institute
> > 44 Binney St.; JF 215

> > Boston, MA 02115

> > Office phone: 617-582-8824
> > Fax: 617-582-8750
> > [hidden email]
> >
> >
> >
> > The information in this e-mail is intended only for the person
> to
> > whom it is
> > addressed. If you believe this e-mail was sent to you in error
> and
> > the e-mail
> > contains patient information, please contact the Partners
> > Compliance HelpLine at
> > http://www.partners.org/complianceline . If the e-mail was sent
> to
> > you in error
> > but does not contain patient information, please contact the
> sender
> > and properly
> > dispose of the e-mail.
> >
>
> Internal Virus Database is out-of-date.
> Checked by AVG.
> Version: 7.5.557 / Virus Database: 270.12.11/2089 - Release Date:

> 30/04/2009 5:53 PM
>
>

Guy Cox

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by RICHARD BURRY

This should not be any problem on (for example) a Prairie or LaVision system fitted on an Olympus stand. I really find it hard to believe that Olympus would not supply this lens for such use. Can anyone actually confirm that they will not? Is there anyone from Olympus on this list?

Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net <http://www.guycox.net/>

________________________________

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of RICHARD BURRY
Sent: Thursday, 14 May 2009 10:47 PM
To: [hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase

The Olympus 25x 1.05 N.A. objective has an enlarged aperture in the back image plane to allow more light throughput. This requires additional lenses capable of filling the aperture, which Olympus has. While the lens may fit on other microscopes, with ut the filling of this aperture, it will not perform as well as it could.

Dick

----- Original Message -----
From: Adrian Smith <[hidden email]>
Date: Thursday, May 14, 2009 8:02 am
Subject: Re: Recommendations for commercial multi-photon system purchase
To: [hidden email]

> I don't believe there is any technical reason why the 25x
> Olympus will
> not work on the LaVision BioTec or Prairie systems.
>
> However, my understanding is that Olympus will only sell
> that
> objective with a new Olympus MPE system... (which makes it a lot
> more
> than just "super expensive" :).
>
> Extremely disappointing decision on Olympus' part.
>
> I would absolutely love to try the objective on our Olympus
> microscope
> (supplied by LaVision BioTec) but I am unable to. If it performs
> as
> well as the marketing material suggests then I would be willing
> to pay
> a premium for it.
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
>
> On 14/05/2009, at 9:23 PM, Watkins, Simon C wrote:
>
> > If and when you try the olympus system make sure they bring
> you the
> > super expensive dipping/coverslip water optic (25X1.12 NA)
> which
> > they designed specifically for 2p. It is absolutely
> spectacular,
> > and pretty much the only lens we use for all our mpe work
> nowadays.
> > When you compare systems it may make you lean towards
> this
> > particular manufacturer. Of course the LaVision and
> Prairie scan
> > heads will fit on the Oly stand, not sure whether they will
> work
> > with this lens but it would be great to find out (anyone out
> there
> > tried this combo yet).
> > S.
> > Simon C. Watkins Ph.D, FRC Path
> > Professor and Vice Chair Cell Biology and Physiology
> > Professor Immunology
> > Director Center for Biologic Imaging
> > BSTS 225
> > University of Pittsburgh
> > 3500 Terrace St
> > Pittsburgh PA 15261
> > 412-352-2277
> > www.cbi.pitt.edu
> >
> > ________________________________________
> > From: Confocal Microscopy List
> [[hidden email]] On
> > Behalf Of Adrian Smith [[hidden email]]
> > Sent: Wednesday, May 13, 2009 8:04 PM
> > To: [hidden email]
> > Subject: Re: Recommendations for commercial multi-photon
> system
> > purchase
> >
> > On 14/05/2009, at 1:57 AM, Alison North wrote:
> >
> >> If you were only interested in multiphoton imaging, the
> LaVision has
> >> certain advantages, but if you need visible lasers too then I'm
> >> afraid it won't suit your purposes.
> >
> > I'm not sure of the details but the LaVision BioTec did
> mention some
> > confocal options to me in passing when we were discussing
> > specifications recently. We were not interested so I didn't
> ask any
> > details but it might be worth clarifying with them what they can
> > currently offer.
> >
> > From recollection I think the Prairie was also MP-only, at
> least when
> > we were looking (that was one of its selling points). Maybe
> that has
> > changed or I'm misrembering?
> >
> > Regards,
> >
> > Adrian Smith
> > Centenary Institute, Sydney, Australia
>
>
> --
> BEGIN-ANTISPAM-VOTING-LINKS
> ------------------------------------------------------
>
> Teach CanIt if this mail (ID 868940829) is spam:
> Spam:
> https://antispam.osu.edu/b.php?c=s&i=868940829&m=73ec3abfd3abNot
> spam: https://antispam.osu.edu/b.php?c=n&i=868940829&m=73ec3abfd3ab
> Forget vote:
> https://antispam.osu.edu/b.php?c=f&i=868940829&m=73ec3abfd3ab----
> --------------------------------------------------
> END-ANTISPAM-VOTING-LINKS
>

Richard W. Burry, Ph.D.
Department of Neuroscience, College of Medicine
Campus Microscopy and Imaging Facility, Director
The Ohio State University
Associate Editor, Journal of Histochemistry and Cytochemistry
277 Biomedical Research Tower
460 West Twelfth Avenue
Columbus, Ohio 43210
Voice 614.292.2814 Cell 614.638.3345 Fax 614.247.8849

Internal Virus Database is out-of-date.
Checked by AVG.
Version: 7.5.557 / Virus Database: 270.12.11/2089 - Release Date: 30/04/2009 5:53 PM

Internal Virus Database is out-of-date.
Checked by AVG.
Version: 7.5.557 / Virus Database: 270.12.11/2089 - Release Date: 30/04/2009 5:53 PM

neeraj Gohad-3

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Cameron Nowell

On the Subject of those black incubators for shielding microscopes from external light, is there a good source for those in the US?

Best,

Neeraj.

Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435

Please Note my NEW Email address: [hidden email]

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cameron Nowell
Sent: Thursday, May 14, 2009 5:22 AM
To: [hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase

Hi Sylvie

The incubators were made in Melbourne by Clear State Solutions (www.clearstatesolutions.com)

Pecon also make them for Leica microscopes (http://www.pecon.biz/?page_id=113)

Cheers

Cam

________________________________

From: Confocal Microscopy List on behalf of Sylvie Le Guyader
Sent: Thu 14/05/2009 6:22 PM
To: [hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase

I think Olympus has now implemented the tuning of the laser and the
compensation within their software.

Cameron, where did you get these black incubators? I am interested in
getting one done as well.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14157 Huddinge
Sweden
+46 (0)8 608 9240

Watkins, Simon C

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Adrian Smith-6

the objective is considerably larger than the standard oly objectives. both in thread diameter and length so modifications of the stand using oly parts is needed too.

Simon C. Watkins Ph.D, FRCPath
Professor and Vice Chair, Cell Biology and Physiology
Professor, Immunology
Director, Center for Biologic Imaging
BSTS 225, University of Pittsburgh
3500 Terrace St.
Pittsburgh PA 15261
Tel: 412-352-2277
Fax:412-648-2797
URL: http://www.cbi.pitt.edu

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Adrian Smith
Sent: Thursday, May 14, 2009 7:58 AM
To: [hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase

I don't believe there is any technical reason why the 25x Olympus will
not work on the LaVision BioTec or Prairie systems.

However, my understanding is that Olympus will only sell that
objective with a new Olympus MPE system... (which makes it a lot more
than just "super expensive" :).

Extremely disappointing decision on Olympus' part.

I would absolutely love to try the objective on our Olympus microscope
(supplied by LaVision BioTec) but I am unable to. If it performs as
well as the marketing material suggests then I would be willing to pay
a premium for it.

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

On 14/05/2009, at 9:23 PM, Watkins, Simon C wrote:

Guy Cox

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Eric Scarfone

The Leica SP2 was not OS9 - it was Win 2000. The older Leica systems were OS9 - a very good OS in its time but there's no denying Leica did persevere with it beyond its use-by date. Nor was it a pico-second laser (that was not very good) we had a femtosecond Coherent Mira. (The Cornell patent was never taken out in Australia).

The funny thing was that in spite of pioneering ND detectors on the 1024, when Bio-Rad brought out their Radiance MP (which was about the time we were making our purchase decision) it did NOT have ND detectors! They introduced them later- and I remember very well one SPIE conference whan they finally had a Radiance MP with ND detectors. They had a lunchtime demo and would not show them - so after the crowd had left I sat down at the system and tried them. Instantly hugely better images than they's been getting in the demo. And they had one potential customer (not me - it was too late for me) who had been desperate to see these detectors in action! You've got it - they had a great product and a keen customer but would not bring them together. No wonder they went under. It was very sad for me - I'd known the people involved from 1987, and had brought two Bio-Rad confocals. But one cannot run a core facility on sentiment.

Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net <http://www.guycox.net/>

________________________________

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Eric Scarfone
Sent: Thursday, 14 May 2009 10:51 PM
To: [hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase

Thanks for the precision Guy. You must be at least as old as I am!
2000 was about the time I installed the Biorad 1024 MP back in France. Did your Leica system actually worked back then (I mean with pico second laser, OS 9 and all?) ;=)

Eric Scarfone, PhD, CNRS,
Center for Hearing and communication Research
Department of Clinical Neuroscience
Karolinska Institutet

Postal Address:
CFH, M1:02
Karolinska Hospital,
SE-171 76 Stockholm, Sweden

Work: +46 (0)8-517 79343,
Cell: +46 (0)70 888 2352
Fax: +46 (0)8-301876

email: [hidden email]
http://www.ki.se/cfh/

----- Original Message -----
From: Guy Cox <[hidden email]>
Date: Thursday, May 14, 2009 3:15 am
Subject: Re: Recommendations for commercial multi-photon system purchase
To: [hidden email]

> Just to keep the record straight, our Leica MP system has non-
> descanneddetectors in both transmission and epi directions - this
> was delivered
> in 2000, ie 9 years ago. We were a fairly early customer for
> these. It
> was (IMHO) a better implementation than the Bio-Rad one (less
> sensitiveto room light). Zeiss back then did have non-descanned
> detection but
> only in transmitted direction - it worked quite well so long as your
> sample wasn't impossibly thick.
>
> Guy
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861 http://www.guycox.net
> ______________________________________________
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]]On Behalf Of Eric Scarfone
> Sent: Thursday, 14 May 2009 12:46 AM
> To: [hidden email]
> Subject: Re: Recommendations for commercial multi-photon system
> purchase
> Hi Lisa
> Lucky you to purchase such a machine!!
>
> The beauty with multiphoton excitation is that optical sectioning
> is
> achieved by the limited volume within which photon density is
> sufficient
> for 2 (or more) quasi simultaneous photon absorption events to
> take
> place. For a given laser setting, the thickness within which this
> occurs
> is only determined by the NA of your objective. ALL the
> fluorescence
> coming from your sample is emitted by this NA determined plane.
> There is
>
> no "out of focus" fluorescence. This is very different from Confocal.
>
> To make full use of this one would like to collect as much of the
> fluorescent photons emitted by the samples as possible. The most
> effective way to do that is to use non-descanned external
> detectors both
>
> in the reflected pathway and in the transmitted pathway.
>
> To my knowledge this was implemented first by Warren Zipfel in
> Watt Webb
>
> lab at Cornell and then commercialized by Bio-Rad on the last
> system
> they put on the market before diseappearing at the end of the
> previous
> century.... After a pretty long absorption/digestion process this
> was
> implemented rather recently by Zeiss.
> Others might have come up with similar solution but I do not know
> about
> it.
>
> I'd were you, for this level of price, I'd ask for an on site test
> with
> your own samples. Do not rely on "in factory" demos!!
>
> Cheers
> Eric
>
>
> Eric Scarfone, PhD, CNRS,
> Center for Hearing and communication Research
> Department of Clinical Neuroscience
> Karolinska Institutet
>
> Postal Address:
> CFH, M1:02
> Karolinska Hospital,
> SE-171 76 Stockholm, Sweden
>
> Work: +46 (0)8-517 79343,
> Cell: +46 (0)70 888 2352
> Fax: +46 (0)8-301876
>
> email: [hidden email]
> http://www.ki.se/cfh/
>
>
> ----- Original Message -----
> From: "Cameron, Lisa" <[hidden email]>
> Date: Wednesday, May 13, 2009 4:14 pm
> Subject: Recommendations for commercial multi-photon system purchase
> To: [hidden email]
>
> > I have been investigating commercial multi-photon systems for
> > awhile in order to
> > purchase a system for my Institute's core microscopy facility.
> Our
> > interest is
> > to have the capability to do intravital imaging on an upright
> > stand, but also be
> > able to have facility users be able to put slides on and use the
> > visible scanner
> > and detectors. I realize this is a tall order for such
> versatility
> > in one
> > system, but since it is for a core (which needs to bring in
> > revenue), I'm
> > looking for the most flexible system. Does anyone have any
> > suggestions about the
> > most recent systems on the market? Or could you point out
> factors
> > you think are
> > the most important for making the decision on which company to
> go
> > with? Please
> > feel free to contact me off line.
> >
> > I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus
> MPE
> > and Prairie's
> > system.
> >
> > (BTW - my own experience is with widefield and confocal live-
> cell
> > imaging, so I
> > have not done 2-p myself, but have been learning about it for
> about
> > a year)
> >
> > Thanks!
> > - Lisa
> >
> > ---------------------------------------
> > Lisa Cameron, Ph.D.
> > Director of Confocal and Light Microscopy
> > Dana Farber Cancer Institute
> > 44 Binney St.; JF 215
> > Boston, MA 02115
> > Office phone: 617-582-8824
> > Fax: 617-582-8750
> > [hidden email]
> >
> >
> >
> > The information in this e-mail is intended only for the person
> to
> > whom it is
> > addressed. If you believe this e-mail was sent to you in error
> and
> > the e-mail
> > contains patient information, please contact the Partners
> > Compliance HelpLine at
> > http://www.partners.org/complianceline . If the e-mail was sent
> to
> > you in error
> > but does not contain patient information, please contact the
> sender
> > and properly
> > dispose of the e-mail.
> >
>
> Internal Virus Database is out-of-date.
> Checked by AVG.
> Version: 7.5.557 / Virus Database: 270.12.11/2089 - Release Date:
> 30/04/2009 5:53 PM
>
>

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Cameron, Lisa

Re: Recommendations for commercial multi-photon system purchase

In reply to this post by Watkins, Simon C

Thanks to all for your comments on commercial 2-p systems.

As for the Olympus objective - the rep from the local distributor (which no
longer is, since Olympus just went direct) said, Olympus has restricted sale of
this "super objective" to people who have or purchase Olympus MPE systems only
because they are (or do not want to) lose to the competition. Also something
about having to manufacture these lens and not having them available for their
own Olympus customers. Seems like if they just stepped up production, they could
make money selling them to whomever...

Zeiss as put some effort into making a lens to compete with this - they have a
20x 1.0 WD=1.8 that they say is competing well. I have not compared these two
objectives directly myself to be able to make any conclusions yet.

- Lisa

---------------------------------------
Lisa Cameron, Ph.D.
Director of Confocal and Light Microscopy
Dana Farber Cancer Institute
44 Binney St.; JF 215
Boston, MA 02115
Office phone: 617-582-8824
Fax: 617-582-8750
Office location: Jimmy Fund Bldg. 220B

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Watkins, Simon C
Sent: Thursday, May 14, 2009 9:17 AM
To: [hidden email]
Subject: Re: [CONFOCALMICROSCOPY] Recommendations for commercial multi-photon
system purchase

the objective is considerably larger than the standard oly objectives. both in
thread diameter and length so modifications of the stand using oly parts is
needed too.

Simon C. Watkins Ph.D, FRCPath
Professor and Vice Chair, Cell Biology and Physiology
Professor, Immunology
Director, Center for Biologic Imaging
BSTS 225, University of Pittsburgh
3500 Terrace St.
Pittsburgh PA 15261
Tel: 412-352-2277
Fax:412-648-2797
URL: http://www.cbi.pitt.edu

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Adrian Smith
Sent: Thursday, May 14, 2009 7:58 AM
To: [hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase

I don't believe there is any technical reason why the 25x Olympus will
not work on the LaVision BioTec or Prairie systems.

However, my understanding is that Olympus will only sell that
objective with a new Olympus MPE system... (which makes it a lot more
than just "super expensive" :).

Extremely disappointing decision on Olympus' part.

I would absolutely love to try the objective on our Olympus microscope
(supplied by LaVision BioTec) but I am unable to. If it performs as
well as the marketing material suggests then I would be willing to pay
a premium for it.

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

On 14/05/2009, at 9:23 PM, Watkins, Simon C wrote:

Guy Cox

Re: Recommendations for commercial multi-photon system purchase

Olympus are certainly not the only people to make such a lens. Leica have one (it's almost the size of a beer can!) but that's not much comfort if you are running a third-party system based on an Olympus stand. I guess there's a caveat emptor here - make sure if you are buying a third-party system on a "Big 4" stand that the microscope manufacturer will warrant to make all new stuff available to you. Or else buy elsewhere.

Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cameron, Lisa
Sent: Thursday, 14 May 2009 11:26 PM
To: [hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase

Thanks to all for your comments on commercial 2-p systems.

As for the Olympus objective - the rep from the local distributor (which no longer is, since Olympus just went direct) said, Olympus has restricted sale of this "super objective" to people who have or purchase Olympus MPE systems only because they are (or do not want to) lose to the competition. Also something about having to manufacture these lens and not having them available for their own Olympus customers. Seems like if they just stepped up production, they could make money selling them to whomever...

Zeiss as put some effort into making a lens to compete with this - they have a 20x 1.0 WD=1.8 that they say is competing well. I have not compared these two objectives directly myself to be able to make any conclusions yet.

- Lisa

---------------------------------------
Lisa Cameron, Ph.D.
Director of Confocal and Light Microscopy Dana Farber Cancer Institute
44 Binney St.; JF 215
Boston, MA 02115
Office phone: 617-582-8824
Fax: 617-582-8750
Office location: Jimmy Fund Bldg. 220B

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Watkins, Simon C
Sent: Thursday, May 14, 2009 9:17 AM
To: [hidden email]
Subject: Re: [CONFOCALMICROSCOPY] Recommendations for commercial multi-photon system purchase

the objective is considerably larger than the standard oly objectives. both in thread diameter and length so modifications of the stand using oly parts is needed too.

Simon C. Watkins Ph.D, FRCPath
Professor and Vice Chair, Cell Biology and Physiology Professor, Immunology Director, Center for Biologic Imaging BSTS 225, University of Pittsburgh 3500 Terrace St.
Pittsburgh PA 15261
Tel: 412-352-2277
Fax:412-648-2797
URL: http://www.cbi.pitt.edu

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Adrian Smith
Sent: Thursday, May 14, 2009 7:58 AM
To: [hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase

I don't believe there is any technical reason why the 25x Olympus will not work on the LaVision BioTec or Prairie systems.

However, my understanding is that Olympus will only sell that objective with a new Olympus MPE system... (which makes it a lot more than just "super expensive" :).

Extremely disappointing decision on Olympus' part.

I would absolutely love to try the objective on our Olympus microscope (supplied by LaVision BioTec) but I am unable to. If it performs as well as the marketing material suggests then I would be willing to pay a premium for it.

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

On 14/05/2009, at 9:23 PM, Watkins, Simon C wrote:

> If and when you try the olympus system make sure they bring you the
> super expensive dipping/coverslip water optic (25X1.12 NA) which they
> designed specifically for 2p. It is absolutely spectacular,
> and pretty much the only lens we use for all our mpe work nowadays.
> When you compare systems it may make you lean towards this particular
> manufacturer. Of course the LaVision and Prairie scan heads will fit
> on the Oly stand, not sure whether they will work with this lens but
> it would be great to find out (anyone out there tried this combo yet).
> S.
> Simon C. Watkins Ph.D, FRC Path
> Professor and Vice Chair Cell Biology and Physiology Professor
> Immunology Director Center for Biologic Imaging BSTS 225 University of
> Pittsburgh 3500 Terrace St Pittsburgh PA 15261
> 412-352-2277
> www.cbi.pitt.edu
>
> ________________________________________
> From: Confocal Microscopy List [[hidden email]] On
> Behalf Of Adrian Smith [[hidden email]]
> Sent: Wednesday, May 13, 2009 8:04 PM
> To: [hidden email]
> Subject: Re: Recommendations for commercial multi-photon system
> purchase
>
> On 14/05/2009, at 1:57 AM, Alison North wrote:
>
>> If you were only interested in multiphoton imaging, the LaVision has
>> certain advantages, but if you need visible lasers too then I'm
>> afraid it won't suit your purposes.
>
> I'm not sure of the details but the LaVision BioTec did mention some
> confocal options to me in passing when we were discussing
> specifications recently. We were not interested so I didn't ask any
> details but it might be worth clarifying with them what they can
> currently offer.
>
> From recollection I think the Prairie was also MP-only, at least when
> we were looking (that was one of its selling points). Maybe that has
> changed or I'm misrembering?
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia

The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.

Internal Virus Database is out-of-date.
Checked by AVG.
Version: 7.5.557 / Virus Database: 270.12.11/2089 - Release Date: 30/04/2009 5:53 PM

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Checked by AVG.
Version: 7.5.557 / Virus Database: 270.12.11/2089 - Release Date: 30/04/2009 5:53 PM

123