Recommendations on Membrane Permeable Dyes

[Nikolaus Naredi-Rainer]

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Dear List,

I am currently looking for a dye (if possible excitable around 488nm) that
is able to penetrate the membrane of giant unilamellar vesicles (GUVs).
These membranes are mainly made of DOPC and similar phospholipid
derivatives.
The aim would be to get a positive control for a fluorescent molecule that
is able to diffuse into the GUV and is accordingly detectable with
Fluorescence Correlation Spectroscopy within the GUV.
So, guessing from literature, I would need a dye that is neither too
hydrophobic nor too lipophobic.
Has anyone of you a dye in mind that is useful for my problem set?
Help is much appreciated!
Thanks in advance,
Niko

—
Dr. Nikolaus Naredi-Rainer
Bioanalytics Group
Department of Biosystems Science and Engineering
ETH Zürich

Vladimir-Prelog-Weg 3, HCI F 301
8093 Zürich
Switzerland

email: [hidden email]
web: http://www.dittrich.ethz.ch/

[Michael Model]

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I think most intracellular probes are amphiphilic. Only if it diffuses
inside it might also diffuse outside unless there the probe is charged
(say, positive) and GUV has a negative potential.

Mike Model

On Fri, Mar 6, 2015 at 4:17 AM, Nikolaus Naredi-Rainer <[hidden email]
> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear List,
>
> I am currently looking for a dye (if possible excitable around 488nm) that
> is able to penetrate the membrane of giant unilamellar vesicles (GUVs).
> These membranes are mainly made of DOPC and similar phospholipid
> derivatives.
> The aim would be to get a positive control for a fluorescent molecule that
> is able to diffuse into the GUV and is accordingly detectable with
> Fluorescence Correlation Spectroscopy within the GUV.
> So, guessing from literature, I would need a dye that is neither too
> hydrophobic nor too lipophobic.
> Has anyone of you a dye in mind that is useful for my problem set?
> Help is much appreciated!
> Thanks in advance,
> Niko
>
> —
> Dr. Nikolaus Naredi-Rainer
> Bioanalytics Group
> Department of Biosystems Science and Engineering
> ETH Zürich
>
> Vladimir-Prelog-Weg 3, HCI F 301
> 8093 Zürich
> Switzerland
>
> email: [hidden email]
> web: http://www.dittrich.ethz.ch/
>

[Michael Giacomelli]

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Most live cell stains have good membrane permeability (which really just
means that they are about equally hydro/lipophilic such that they diffuse
through a lipid membrane without having enough affinity to become trapped
there).   The acridine family is a good choice though, since they are 488
excitable, highly membrane permeable, and quite bright.  If you have
acridine orange or acriflavine around, I would try those first.

Mike


On Fri, Mar 6, 2015 at 11:59 AM, Michael Model <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I think most intracellular probes are amphiphilic. Only if it diffuses
> inside it might also diffuse outside unless there the probe is charged
> (say, positive) and GUV has a negative potential.
>
> Mike Model
>
> On Fri, Mar 6, 2015 at 4:17 AM, Nikolaus Naredi-Rainer <

[hidden email]

>> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear List,
>>
>> I am currently looking for a dye (if possible excitable around 488nm)

that
>> is able to penetrate the membrane of giant unilamellar vesicles (GUVs).
>> These membranes are mainly made of DOPC and similar phospholipid
>> derivatives.
>> The aim would be to get a positive control for a fluorescent molecule
that

>> is able to diffuse into the GUV and is accordingly detectable with
>> Fluorescence Correlation Spectroscopy within the GUV.
>> So, guessing from literature, I would need a dye that is neither too
>> hydrophobic nor too lipophobic.
>> Has anyone of you a dye in mind that is useful for my problem set?
>> Help is much appreciated!
>> Thanks in advance,
>> Niko
>>
>> —
>> Dr. Nikolaus Naredi-Rainer
>> Bioanalytics Group
>> Department of Biosystems Science and Engineering
>> ETH Zürich
>>
>> Vladimir-Prelog-Weg 3, HCI F 301
>> 8093 Zürich
>> Switzerland
>>
>> email: [hidden email]
>> web: http://www.dittrich.ethz.ch/
>>