*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
On May 4, 2011, at 9:41 AM, Katherina Garcia Garcia wrote:
>
> We are going to buy a Multiphoton Microscope and we would like to
> add a laser ablation module to this system. One company offers us to
> do the photoablation with the same laser we do multiphoton imaging.
>
>
> Do you have experience doing photoablation with a multiphoton laser?
>
Dear Katherine,
Two few years ago, we were doing laser ablation on the actin cortex of
floating and adhering fibroblasts.
Jan Peychl set up an Olympus FlowView 1000, with the second scanner
coupled to a 405nm PicoQuant pulsed laser (single photon ablation). We
were happy with it: ablation was gentle enough for the cell to
recover, and we had a large range of power for which the ablation
effect could be tuned.
We also tried doing ablation on the samples on Nicola Maghelli's
homemade 2 photons, using the imaging infrared pulsed laser.
Though our experience was very limited, we had a very hard time
finding suitable parameters for the ablation to occur. Depending on
the output power, we had either no effect (or bleaching), either the
whole cell exploded. The parameters set for which the cortex was
ablated was very sensitive, and once we have found it for one cell, it
would not work for another cell.
So we did not pursue in this direction, and went back to single-photon
ablation.
Cheers
jy
--
Jean-Yves Tinevez
PFID - Imagopole
Institut Pasteur
25-28, rue du Docteur Roux
75724 Paris cedex 15
France
tel: +33 1 40 61 35 40
Locked
