Reflex microscope
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Boswell, Carl A - (cboswell) |
Reflex microscope
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I know someone at Arizona State University (Phoenix) that is looking for a
Reflex microscope to look at surface details of dental specimens. Anyone with experience with one, and know where one might be found? Thanks, carl Carl A. Boswell, Ph.D. Molecular and Cellular Biology University of Arizona 520-954-7053 FAX 520-621-3709 |
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I think any of the confocals in the market have a reflection mode and are
capable of getting a topographical image of a surface with this. At least topographical images of silicone skin replicas has been done with a standard Leica SP2. There are also more specialised equipment to do this, like the PLµ confocal profilometer by Sensofar, http://www.sensofar.com/products.html (no commercial interest) Best, Xavi. ___________________________________ Xavier Sanjuan Servei de Microscòpia Confocal Departament de Ciències Experimentals i de la Salut Universitat Pompeu Fabra Parc de Recerca Biomèdica de Barcelona Doctor Aiguader, 88 - Sala 309 08003 Barcelona - Spain Tel.: + 34 93 316 08 64 Fax: + 34 93 316 09 01 E-mail: [hidden email] Web: http://www.upf.edu/sct -----Mensaje original----- De: Confocal Microscopy List [mailto:[hidden email]] En nombre de Carl Boswell Enviado el: martes, 20 de enero de 2009 18:59 Para: [hidden email] Asunto: Reflex microscope I know someone at Arizona State University (Phoenix) that is looking for a Reflex microscope to look at surface details of dental specimens. Anyone with experience with one, and know where one might be found? Thanks, carl Carl A. Boswell, Ph.D. Molecular and Cellular Biology University of Arizona 520-954-7053 FAX 520-621-3709 |
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care should be executed while sample preparation on what is the resolution you are looking for if u neeed a higher resolution your working distance is low cut the sample enough from the bottom so that u get max magnification without compromising on resolution.
On Wed, Jan 21, 2009 at 4:16 PM, Xavier Sanjuan <[hidden email]> wrote: I think any of the confocals in the market have a reflection mode and are |
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care should be executed while sample preparation on what is the resolution you are looking for if u neeed a higher resolution your working distance is low cut the sample enough from the bottom so that u get max magnification without compromising on resolution. |
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In reply to this post by Boswell, Carl A - (cboswell)
I have done topographical imaging of tooth replicas, made with fluorescently
stained dental epoxy. I have used Olympus FV1000 and the 40x/0.6 long working distance objective; Fluorescence mode worked better than the reflective mode in my case, because the surface reflectivity was very uneven. Topographical projections were made in ImageJ with TopoJ plugin - this gave a better output than the TOPO projection function built in the Olympus confocal software. The results were OK; I have not measured the z-resolution of the topographical map, but my guesstimate is that it is somewhere between 20 and 100 nm. An example (of the reflective mode and a 3D test specimen) is shown on our web site: http://microscopy.tamu.edu/picture-of-the- month.html (scorll down to April 2008). The biggest problem was the speed of acquisition - the need to scan large areas with lots of pixels, plus doing 50 nm or smaller z-step over several mm depth quickly made it impractical or impossible to do imaging for anything more than just a handful of samples. The spinning-disk confocal profilometers are much much faster. besides the Sensofar system mentioned before, I know that Zeiss and Nanofocus have such spinning disk systems. Regards, Stan Stanislav Vitha, Ph.D. Microscopy and Imaging Center Texas A&M University BSBW 119 College Station, TX 77843-2257 http://microscopy.tamu.edu On Wed, 21 Jan 2009 18:56:38 +0530, regan m <[hidden email]> wrote: >care should be executed while sample preparation on what is the resolution >you are looking for if u neeed a higher resolution your working distance is >low cut the sample enough from the bottom so that u get max magnification >without compromising on resolution. > >On Wed, Jan 21, 2009 at 4:16 PM, Xavier Sanjuan <[hidden email]>wrote: > >> I think any of the confocals in the market have a reflection mode and are >> capable of getting a topographical image of a surface with this. At least >> topographical images of silicone skin replicas has been done with a >> standard >> Leica SP2. >> >> There are also more specialised equipment to do this, like the PLµ confocal >> profilometer by Sensofar, http://www.sensofar.com/products.html (no >> commercial interest) >> >> Best, >> >> Xavi. >> >> ___________________________________ >> >> Xavier Sanjuan >> Servei de Microscòpia Confocal >> Departament de Ciències Experimentals i de la Salut >> Universitat Pompeu Fabra >> Parc de Recerca Biomèdica de Barcelona >> Doctor Aiguader, 88 - Sala 309 >> 08003 Barcelona - Spain >> >> Tel.: + 34 93 316 08 64 >> Fax: + 34 93 316 09 01 >> E-mail: [hidden email] >> Web: http://www.upf.edu/sct >> >> -----Mensaje original----- >> De: Confocal Microscopy List >> nombre de Carl Boswell >> Enviado el: martes, 20 de enero de 2009 18:59 >> Para: [hidden email] >> Asunto: Reflex microscope >> >> I know someone at Arizona State University (Phoenix) that is looking for a >> Reflex microscope to look at surface details of dental specimens. Anyone >> with experience with one, and know where one might be found? >> >> Thanks, >> carl >> >> Carl A. Boswell, Ph.D. >> Molecular and Cellular Biology >> University of Arizona >> 520-954-7053 >> FAX 520-621-3709 >> > |
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