Refractive Index Question

[Neil Anthony]

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Hi all, I hope the scopes treat you well.

I was wondering if there's any kind of material, thin film, plastic, nano mesh, composite etc etc anything that has a similar refractive index to live cell imaging media.  I believe the refractive index of PBS is 1.334 ish and something like DMEM is a little higher, maybe 1.34.

I would like to find some that I can use to constrain non-adherent cells as close to the coverslip as possible explicitly for a water dipping lens.  The idea would be to sandwich (possibly pulling it tight around something like cling film) the cells on a coverslip.

Does anybody know of any material that might come close?

Thanks in advance for your time.

Neil



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[mmodel]

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Hi Neil,

I don't know of any solid material with n = 1.334 (by the way, the refractive indices of PBS, DMEM and of water are practically the same). Plastics have n close to 1.5. Proteins increase n by ~0.19 per g/mL.

But perhaps you could stabilize cells with poly-l-lys or some other sticky coating.

Mike

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Anthony, Neil
Sent: Wednesday, August 16, 2017 1:43 PM
To: [hidden email]
Subject: Refractive Index Question

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Hi all, I hope the scopes treat you well.

I was wondering if there's any kind of material, thin film, plastic, nano mesh, composite etc etc anything that has a similar refractive index to live cell imaging media.  I believe the refractive index of PBS is 1.334 ish and something like DMEM is a little higher, maybe 1.34.

I would like to find some that I can use to constrain non-adherent cells as close to the coverslip as possible explicitly for a water dipping lens.  The idea would be to sandwich (possibly pulling it tight around something like cling film) the cells on a coverslip.

Does anybody know of any material that might come close?

Thanks in advance for your time.

Neil



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[Mark A. Sanders]

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Hi Neil,

You may want to consider CyGEL (http://www.biostatus.com/CyGel/ <http://www.biostatus.com/CyGel/>). I believe that it has an R=~1.37.

No commercial interests…


Cheers,
Mark
************************************************
Mark A. Sanders      University of Minnesota
Program Director      Twin Cities Campus
University Imaging Centers  
Nikon Center of Excellence <https://www.nikoninstruments.com/Imaging-Centers/Nikon-Centers-of-Excellence/Nikon-CofE-Locations/Americas/University-of-Minnesota>
www.uic.umn.edu <http://www.uic.umn.edu/>
http://uic.umn.edu/content/locations <http://uic.umn.edu/content/locations>

 

> On Aug 16, 2017, at 12:42 PM, Anthony, Neil <[hidden email]> wrote:
>
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> *****
>
> Hi all, I hope the scopes treat you well.
>
> I was wondering if there's any kind of material, thin film, plastic, nano mesh, composite etc etc anything that has a similar refractive index to live cell imaging media.  I believe the refractive index of PBS is 1.334 ish and something like DMEM is a little higher, maybe 1.34.
>
> I would like to find some that I can use to constrain non-adherent cells as close to the coverslip as possible explicitly for a water dipping lens.  The idea would be to sandwich (possibly pulling it tight around something like cling film) the cells on a coverslip.
>
> Does anybody know of any material that might come close?
>
> Thanks in advance for your time.
>
> Neil
>
>
>
> ________________________________
>
> This e-mail message (including any attachments) is for the sole use of
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[Eric Girard]

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Could this work?

https://www.google.ca/url?sa=t&source=web&rct=j&url=https://arxiv.org/pdf/1212.0820&ved=0ahUKEwjY0q-4rNzVAhWf14MKHUuOBfAQFgghMAA&usg=AFQjCNGoFUNelU07maMIyDJzpbQfYOFHZg


Eric Girard


Le 16 août 2017 1:42 PM, "Anthony, Neil" <[hidden email]> a écrit :

> *****
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> *****
>
> Hi all, I hope the scopes treat you well.
>
> I was wondering if there's any kind of material, thin film, plastic, nano
> mesh, composite etc etc anything that has a similar refractive index to
> live cell imaging media.  I believe the refractive index of PBS is 1.334
> ish and something like DMEM is a little higher, maybe 1.34.
>
> I would like to find some that I can use to constrain non-adherent cells
> as close to the coverslip as possible explicitly for a water dipping lens.
> The idea would be to sandwich (possibly pulling it tight around something
> like cling film) the cells on a coverslip.
>
> Does anybody know of any material that might come close?
>
> Thanks in advance for your time.
>
> Neil
>
>
>
> ________________________________
>
> This e-mail message (including any attachments) is for the sole use of
> the intended recipient(s) and may contain confidential and privileged
> information. If the reader of this message is not the intended
> recipient, you are hereby notified that any dissemination, distribution
> or copying of this message (including any attachments) is strictly
> prohibited.
>
> If you have received this message in error, please contact
> the sender by reply e-mail message and destroy all copies of the
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>

[Alberto Diaspro]

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Gradient of glucose concentration allow to have gradients of refractive index from 1.33 to approx. 1.47.
I used it for performing some contrast matching against nucleus. Redundant to say that increasing concentration viscosity changes.
Maybe you can be interested in
https://www.researchgate.net/publication/11376486_Influence_of_Refractive-Index_Mismatch_in_High-Resolution_Three-Dimensional_Confocal_Microscopy
Best
Alby


Alberto iPad

> Il giorno 16 ago 2017, alle ore 19:42, Anthony, Neil <[hidden email]> ha scritto:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all, I hope the scopes treat you well.
>
> I was wondering if there's any kind of material, thin film, plastic, nano mesh, composite etc etc anything that has a similar refractive index to live cell imaging media.  I believe the refractive index of PBS is 1.334 ish and something like DMEM is a little higher, maybe 1.34.
>
> I would like to find some that I can use to constrain non-adherent cells as close to the coverslip as possible explicitly for a water dipping lens.  The idea would be to sandwich (possibly pulling it tight around something like cling film) the cells on a coverslip.
>
> Does anybody know of any material that might come close?
>
> Thanks in advance for your time.
>
> Neil
>
>
>
> ________________________________
>
> This e-mail message (including any attachments) is for the sole use of
> the intended recipient(s) and may contain confidential and privileged
> information. If the reader of this message is not the intended
> recipient, you are hereby notified that any dissemination, distribution
> or copying of this message (including any attachments) is strictly
> prohibited.
>
> If you have received this message in error, please contact
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[Rusty Nicovich]

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MY Polymers makes a number of optical adhesives with refractive indices
very close to cell medium.

http://www.mypolymers.com/products

These have been used to make polymer chips for holding live cells for
imaging and for the basis of the soSPIM microscope.  Virgile Viasnoff at
NUS is the chip expert, with JB Sibarita and Remi Galland the optics
experts on the team that has published those.  You would have to mold your
own chambers, but on the other hand you could mold your own chambers + UV
cure.

FEP (fluorinated ethylene propylene) tubing is used for sample holding in
light sheet microscopy.  That plastic has a RI of ~1.34.  A number of
vendors sell sheets or films of this for not much money that should do what
you're looking for.

Thanks,
Rusty

On Wed, Aug 16, 2017 at 11:11 AM, Eric Girard <[hidden email]>
wrote:

> *****
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> *****
>
> Could this work?
>
> https://www.google.ca/url?sa=t&source=web&rct=j&url=https:/
> /arxiv.org/pdf/1212.0820&ved=0ahUKEwjY0q-4rNzVAhWf14MKHUuOBfAQFgghMAA&usg=
> AFQjCNGoFUNelU07maMIyDJzpbQfYOFHZg
>
>
> Eric Girard
>
>
> Le 16 août 2017 1:42 PM, "Anthony, Neil" <[hidden email]> a écrit :
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi all, I hope the scopes treat you well.
> >
> > I was wondering if there's any kind of material, thin film, plastic, nano
> > mesh, composite etc etc anything that has a similar refractive index to
> > live cell imaging media.  I believe the refractive index of PBS is 1.334
> > ish and something like DMEM is a little higher, maybe 1.34.
> >
> > I would like to find some that I can use to constrain non-adherent cells
> > as close to the coverslip as possible explicitly for a water dipping
> lens.
> > The idea would be to sandwich (possibly pulling it tight around something
> > like cling film) the cells on a coverslip.
> >
> > Does anybody know of any material that might come close?
> >
> > Thanks in advance for your time.
> >
> > Neil
> >
> >
> >
> > ________________________________
> >
> > This e-mail message (including any attachments) is for the sole use of
> > the intended recipient(s) and may contain confidential and privileged
> > information. If the reader of this message is not the intended
> > recipient, you are hereby notified that any dissemination, distribution
> > or copying of this message (including any attachments) is strictly
> > prohibited.
> >
> > If you have received this message in error, please contact
> > the sender by reply e-mail message and destroy all copies of the
> > original message (including attachments).
> >
>

[Gary Laevsky]

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Lumox (Sarstedt) might work.  Gas permeable, fluid impermeable.  Film-like
...

On Wed, Aug 16, 2017 at 2:34 PM, Alberto Diaspro <[hidden email]> wrote:

> *****
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> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> Gradient of glucose concentration allow to have gradients of refractive
> index from 1.33 to approx. 1.47.
> I used it for performing some contrast matching against nucleus. Redundant
> to say that increasing concentration viscosity changes.
> Maybe you can be interested in
> https://www.researchgate.net/publication/11376486_
> Influence_of_Refractive-Index_Mismatch_in_High-Resolution_
> Three-Dimensional_Confocal_Microscopy
> Best
> Alby
>
>
> Alberto iPad
>
> > Il giorno 16 ago 2017, alle ore 19:42, Anthony, Neil <[hidden email]>
> ha scritto:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi all, I hope the scopes treat you well.
> >
> > I was wondering if there's any kind of material, thin film, plastic,
> nano mesh, composite etc etc anything that has a similar refractive index
> to live cell imaging media.  I believe the refractive index of PBS is 1.334
> ish and something like DMEM is a little higher, maybe 1.34.
> >
> > I would like to find some that I can use to constrain non-adherent cells
> as close to the coverslip as possible explicitly for a water dipping lens.
> The idea would be to sandwich (possibly pulling it tight around something
> like cling film) the cells on a coverslip.
> >
> > Does anybody know of any material that might come close?
> >
> > Thanks in advance for your time.
> >
> > Neil
> >
> >
> >
> > ________________________________
> >
> > This e-mail message (including any attachments) is for the sole use of
> > the intended recipient(s) and may contain confidential and privileged
> > information. If the reader of this message is not the intended
> > recipient, you are hereby notified that any dissemination, distribution
> > or copying of this message (including any attachments) is strictly
> > prohibited.
> >
> > If you have received this message in error, please contact
> > the sender by reply e-mail message and destroy all copies of the
> > original message (including attachments).
>

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

[Dan Focht]

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Neil


You could look into Glycosan from ESI BIO.
http://www.esibio.com/about-us/our-technology/

Another possibility is to use a sperm slide with a fixed micron spacing.
They load by capillary action and are available from a number of suppliers such as Cellvu.com.
If you need supplemental environmental control call me!


Dan


On Aug 16, 2017, at 1:42 PM, Anthony, Neil wrote:

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Hi all, I hope the scopes treat you well.

I was wondering if there's any kind of material, thin film, plastic, nano mesh, composite etc etc anything that has a similar refractive index to live cell imaging media.  I believe the refractive index of PBS is 1.334 ish and something like DMEM is a little higher, maybe 1.34.

I would like to find some that I can use to constrain non-adherent cells as close to the coverslip as possible explicitly for a water dipping lens.  The idea would be to sandwich (possibly pulling it tight around something like cling film) the cells on a coverslip.

Does anybody know of any material that might come close?

Thanks in advance for your time.

Neil


________________________________

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Dan Focht
Bioptechs Inc.
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Butler, PA 16002-9259
 
Office: 724-282-7145
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[hidden email]
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[Julie-Christine Lévesque]

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*No commercial interest

Hi Neil,
You might try CyGEL.. It is specified that the refractive index is 1.37.
Best,


Julie-Christine Levesque, M.Sc.
Bioimaging platform manager
Centre de recherche en infectiologie, local R-2749.5
CRCHU de Québec, pavillon CHUL

[hidden email]<mailto:[hidden email]>


Le 16 août 2017 à 13:42, Anthony, Neil <[hidden email]<mailto:[hidden email]>> a écrit :

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Hi all, I hope the scopes treat you well.

I was wondering if there's any kind of material, thin film, plastic, nano mesh, composite etc etc anything that has a similar refractive index to live cell imaging media.  I believe the refractive index of PBS is 1.334 ish and something like DMEM is a little higher, maybe 1.34.

I would like to find some that I can use to constrain non-adherent cells as close to the coverslip as possible explicitly for a water dipping lens.  The idea would be to sandwich (possibly pulling it tight around something like cling film) the cells on a coverslip.

Does anybody know of any material that might come close?

Thanks in advance for your time.

Neil



________________________________

This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
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[Alberto Diaspro]

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About glucose

https://www.researchgate.net/figure/258717603_fig1_Fig-3-Refractive-index-of-the-aqueous-solution-of-glucose-versus-glucose-concentration
Best
Alby

Alberto iPad

> Il giorno 16 ago 2017, alle ore 19:42, Anthony, Neil <[hidden email]> ha scritto:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all, I hope the scopes treat you well.
>
> I was wondering if there's any kind of material, thin film, plastic, nano mesh, composite etc etc anything that has a similar refractive index to live cell imaging media.  I believe the refractive index of PBS is 1.334 ish and something like DMEM is a little higher, maybe 1.34.
>
> I would like to find some that I can use to constrain non-adherent cells as close to the coverslip as possible explicitly for a water dipping lens.  The idea would be to sandwich (possibly pulling it tight around something like cling film) the cells on a coverslip.
>
> Does anybody know of any material that might come close?
>
> Thanks in advance for your time.
>
> Neil
>
>
>
> ________________________________
>
> This e-mail message (including any attachments) is for the sole use of
> the intended recipient(s) and may contain confidential and privileged
> information. If the reader of this message is not the intended
> recipient, you are hereby notified that any dissemination, distribution
> or copying of this message (including any attachments) is strictly
> prohibited.
>
> If you have received this message in error, please contact
> the sender by reply e-mail message and destroy all copies of the
> original message (including attachments).

[Sven Terclavers-3]

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Hi Neil,

For Lightsheet live cell imaging sometimes FEP tubes are used as they have similar RI. Maybe that's something?
Best regards,

Sven

Sent from my iPhone

> On Aug 16, 2017, at 13:42, Anthony, Neil <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all, I hope the scopes treat you well.
>
> I was wondering if there's any kind of material, thin film, plastic, nano mesh, composite etc etc anything that has a similar refractive index to live cell imaging media.  I believe the refractive index of PBS is 1.334 ish and something like DMEM is a little higher, maybe 1.34.
>
> I would like to find some that I can use to constrain non-adherent cells as close to the coverslip as possible explicitly for a water dipping lens.  The idea would be to sandwich (possibly pulling it tight around something like cling film) the cells on a coverslip.
>
> Does anybody know of any material that might come close?
>
> Thanks in advance for your time.
>
> Neil
>
>
>
> ________________________________
>
> This e-mail message (including any attachments) is for the sole use of
> the intended recipient(s) and may contain confidential and privileged
> information. If the reader of this message is not the intended
> recipient, you are hereby notified that any dissemination, distribution
> or copying of this message (including any attachments) is strictly
> prohibited.
>
> If you have received this message in error, please contact
> the sender by reply e-mail message and destroy all copies of the
> original message (including attachments).

[Jason Miller]

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Fluorocarbon filmed plates have refractive index of nearly water. I wonder if this material could help?

http://www.coylab.com/wp-content/uploads/2016/05/Perm-Plates-Lit-101811WR.pdf


-------

Jason Miller, MD, PhD

University of Michigan Kellogg Eye Center



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E-mail: [hidden email]<mailto:[hidden email]>



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On Wed, Aug 16, 2017 at 1:42 PM, Anthony, Neil <[hidden email]<mailto:[hidden email]>> wrote:
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Hi all, I hope the scopes treat you well.

I was wondering if there's any kind of material, thin film, plastic, nano
mesh, composite etc etc anything that has a similar refractive index to
live cell imaging media.  I believe the refractive index of PBS is 1.334
ish and something like DMEM is a little higher, maybe 1.34.

I would like to find some that I can use to constrain non-adherent cells
as close to the coverslip as possible explicitly for a water dipping lens.
The idea would be to sandwich (possibly pulling it tight around something
like cling film) the cells on a coverslip.

Does anybody know of any material that might come close?

Thanks in advance for your time.

Neil



________________________________

This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly
prohibited.

If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
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[Neil Anthony]

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Thank you all for your responses!  What great avenues for me to explore.  How I love the confocal listserv!

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sven Terclavers
Sent: Wednesday, August 16, 2017 4:23 PM
To: [hidden email]
Subject: Re: Refractive Index Question

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Hi Neil,

For Lightsheet live cell imaging sometimes FEP tubes are used as they have similar RI. Maybe that's something?
Best regards,

Sven

Sent from my iPhone

> On Aug 16, 2017, at 13:42, Anthony, Neil <[hidden email]> wrote:
>
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> Hi all, I hope the scopes treat you well.
>
> I was wondering if there's any kind of material, thin film, plastic, nano mesh, composite etc etc anything that has a similar refractive index to live cell imaging media.  I believe the refractive index of PBS is 1.334 ish and something like DMEM is a little higher, maybe 1.34.
>
> I would like to find some that I can use to constrain non-adherent cells as close to the coverslip as possible explicitly for a water dipping lens.  The idea would be to sandwich (possibly pulling it tight around something like cling film) the cells on a coverslip.
>
> Does anybody know of any material that might come close?
>
> Thanks in advance for your time.
>
> Neil
>
>
>
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[daj1u06]

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if you don't need a physical barrier, you could try pretreating the containing vessel with 1% alcian blue in dH2O for about 5 mins and then rinsing till you don't get any more blue washing out. Add cells and simply let them settle and stick. We use it routinely for imaging lymphoma cells in glass bottom chamber slides on our inverted confocal (have never tried with plastic bottom vessels).
Dave Johnston,
Biomedical Imaging Unit, Southampton UK.