Register Today for CYTO U's latest webinar: The Use of Nanoparticles for Cell Identification and Tracking on April 16!

[Pulliam, Kanika F.]

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The Use of Nanoparticles for Cell Identification and Tracking



Presented by

Paul Rees



Professor, College of Engineering

Swansea University



April 16, 2015

6:00 am PT US/Canada
9:00 am ET US/Canada
2:00 pm GMT/UK/Portugal
3:00 pm CET/Berlin

Register: http://cytou.peachnewmedia.com
Cost: FREE

Paul Rees received his degree and Ph.D. in physics from Cardiff University in 1990 and 1993, respectively. Research for his PhD included the calculation of the optical properties of semiconductor lasers and the measurement of spontaneous emission from laser diodes under operating conditions. From 1994 to 1996, he was a Research Fellow at the Department of Physics, Trinity College, Dublin, Ireland, where his research included the study of self-pulsation in laser diodes and the theory of many-body effects in wide band-gap semiconductors. In 1996, he joined the School of Informatics, University of Wales, Bangor, U.K., where he was appointed Senior Lecturer in 2000. Research interests at Bangor included the effect of optical feedback in laser diodes, the use of chaos for data encryption, quantum computing and the theory of quantum-dot lasers.

In 2005 he was appointed to a Chair of Nanotechnology in the newly-formed Multidisciplinary Nanotechnology Centre at Swansea University. Current research interests include the theory of the electrical and optical properties of self assembled and colloidal semiconductor quantum dots. Recent research has involved the modelling of biological cell cycles and the use of these theories in deconvolving useful information from high throughput measurement techniques such as flow cytometry and time lapse microscopy. A major focus of current research is the investigation of the uptake of nano and micro-particles in biological cells. Using the high throughput measurement techniques to investigate the heterogeneity of this uptake for large cell populations, allowing the calculation of the statistical distributions for the number particles delivered to a cell. Recent research has involved the application of engineering systems approaches to both biology and medicine in order to deconvolve meaningful information from data.

Webinar Summary:
Colloidal quantum dots are widely used as fluorescent markers for biological cells.  These particles are optically stable and bio-compatible with the cellular environment. The wavelength of the quantum dot fluorescence can be tuned across the optical spectrum by varying the size, structure and chemical composition of the colloidal particles. Initially, colloidal dots were used as direct replacements of existing fluorescence probes but more recently applications have emerged that exploit the unique properties of these nanoparticles.

In this presentation, Paul will discuss the use of colloidal nanoparticles to measure the proliferation of cells using flow cytometry. Then the use of nanoparticles to track the movement of cells using a fluorescence microscope will be presented and finally he will outline the use of multi-colored nanoparticles to uniquely bar code cells for identification and tracking.


CYTO U Webinar Recordings
A recording of this webinar will be posted online at CYTO U within 24-48 hours after the live event for free viewing by all.  You may also want to watch the recordings of CYTO U's last three webinars Forensic Flow Cytometry by Jennifer Wilshire, Standardization of Flow Cytometry Assays: What for? How? Where does it lead us? by Tomáš Kalina, and Growing a Successful and Fruitful Core: You Reap What You Sow by Joanne Lannigan at CYTO U.  For more details, visit: http://cytou.peachnewmedia.com.

CYTO University
CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, and interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all. Learn more at http://cytou.peachnewmedia.com

CYTO U Access for ISAC Members
For current ISAC members, your user login and password to CYTO U are the same user login and password you have for the members-only section of the ISAC Web site. If you remember your user log in to the ISAC Web site but cannot remember your password, you can obtain your password from the ISAC Web site by clicking on the link "forgot your password?".  If you cannot remember your username, you can contact [hidden email] for your login information.


____________________
Kanika F. Pulliam, Ph.D.
Education Manager
International Society for Advancement of Cytometry (ISAC)
9650 Rockville Pike
Bethesda, MD 20814
[phone]
[hidden email]<mailto:[hidden email]>