Release of OMERO & Bio-Formats 5.0.4

[Jason Swedlow-2]

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Dear All,

Today we are launching 5.0.4, a bug-fixing release.

For OMERO this includes:
-- fixes for Java 8 issues - both the OMERO clients and OMERO.server should
now be compatible with this Java update
-- a fix for uploading masks in OMERO.matlab

Bio-Formats improvements include:
-- multiple fixes for the .ND2 formats
-- Java 8 fixes
-- support for PicoQuant .bin files (thanks to Ian Munro)


Full details are available on the OMERO Github milestone[1] and the
Bio-Formats Github milestone[2] pages.

The software is available from
http://downloads.openmicroscopy.org/omero/5.0.4/
and
http://downloads.openmicroscopy.org/bio-formats/5.0.4/

For information on upgrading your server, see the upgrade guide for system
administrators -
http://www.openmicroscopy.org/site/support/omero5/sysadmins/server-upgrade.html
Note that you need to upgrade your OMERO.server to take advantage of the
improved support for .ND2 in Bio-Formats.

For full details of the next major release, see the 5.1 milestone page-
http://trac.openmicroscopy.org.uk/ome/milestone/5.1.0
We currently intend 5.0.4 to be the last update to the 5.0.x series. Our
development focus is now on the OME 5.1 line and updating the OME Data
Model for the next schema release, and while we will continue to support
both 4.4.11 and 5.0.4 through early 2015, we will only perform critical bug
fixes on the 5.0.x line and do not intend any fixes on the 4.4.x line.

Kind regards,
The OME team


[1]
https://github.com/openmicroscopy/openmicroscopy/issues?q=milestone%3A5.0.4+is%3Aclosed
[2]
https://github.com/openmicroscopy/bioformats/issues?q=milestone%3A5.0.4+is%3Aclosed


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Centre for Gene Regulation & Expression
College of Life Sciences
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[Michael Abanto]

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Hi,

I have a user who wants to do live cell imaging and then fix, stain and re-image. The idea is to track the cell first while it's alive, and then check out it's expression etc after. The problem is that the cells are not very adherent and they detach easily if any fluid is changed, e.g. during subsequent washing/staining.

So, does anyone know of a method to sufficiently immobilize the cells after a live-cell experiment so that they can be post fixed and stained without being washed away?

The user has had very limited success with PLL, and I wondered if someone had some clever (and preferably low cost/easily implemented) single cell trick.

Thanks for any reply,

Mike