Removable glue for optics
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all, I apologyze for this not real confocal question... I am recently putting mi hands on a Olympus IX81 equipped for fluorescence image acquisition and I would like to move into deconvolution of wide field images. I am asking If any of you tried this technique on this microscope, and the minimal optical/software configuration suggested (I have a light guide stable illumination, filter wheel for excitation filters, good CCD camera, vibration free environment, unfotunately not UPLANSAPO objectives at the moment) I wonder also If the Z axis motorized movement is accurate enough for the purpose, the company says 10 nm steps... Thanks, best regards Stefano Stefano Giovannardi PhD DiSTA - BRD Università dell'Insubria via J.H. Dunant 3 21100 Varese Italy [hidden email] |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Stefano, The IX81 is a standard configuration on many Olympus confocals. The z-focus is sufficient for optical volumes. If the scope is old and heavily used, it may need repair, which is much less than buying a new scope. Our FV-1000 has about 20,000 hours of use and the IX81 is still very solid. Does the illumination automation include shutter control? the lack of UPLANSAPO lenses are not a deal breaker depending on the wavelengths with which you will be working and possessing sufficient NA. You can use PSF beads (which you need anyway), ring beads or reflections to test the field flatness and chromatic aberrations in the lenses you do have. Such samples can also be used to measure any stage drift or focus problems by running 2D and 3D timelapses. If the camera is mounted with 2X doubling, that will reduce the field curvature. Increasing mag to the camera would help with curvature and maybe improve resolution. The 2 leading commercial software apps for deconvolution are Huygens (SVI, NL) and Autodeblur (Autoquant, USA). Each has its adherents, I'm happy with Huygens. Regards, Glen Glen MacDonald Cellular Morphology Core Center for Human Development and Disability Box 357920 University of Washington Seattle, WA 98195-7920 USA (206) 616-4156 [hidden email] On Jun 27, 2012, at 4:45 AM, Stefano Giovannardi wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear all, > > I apologyze for this not real confocal question... > I am recently putting mi hands on a Olympus IX81 equipped for fluorescence image acquisition and I would like to move into deconvolution of wide field images. > I am asking If any of you tried this technique on this microscope, and the minimal optical/software configuration suggested (I have a light guide stable illumination, filter wheel for excitation filters, good CCD camera, vibration free environment, unfotunately not UPLANSAPO objectives at the moment) > I wonder also If the Z axis motorized movement is accurate enough for the purpose, the company says 10 nm steps... > > Thanks, best regards > Stefano > > Stefano Giovannardi PhD > DiSTA - BRD > Università dell'Insubria > via J.H. Dunant 3 > 21100 Varese Italy > [hidden email] |
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In reply to this post by Stefano Giovannardi
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Ciao Stefano, I could suggest contact Dr Javier Adur (Lab Dra. María Fernanda Izaguirre) from Univ Entre Rios, Argentina, at [hidden email] ( http://www.bioingenieria.edu.ar/grupos/microscopia/MicroInicio.html). I know they have made such a Z- motorization in an Olympus microscope (sorry, no remember model) to perform deconvolution. Regards, José Pellegrino Inst Exptl Physiol CONICET - Natl Univ Rosario ________________________________________ De: Confocal Microscopy List [[hidden email]] En nombre de Stefano Giovannardi [[hidden email]] Enviado el: miércoles, 27 de junio de 2012 08:45 a.m. Para: [hidden email] Asunto: deconvolution ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all, I apologyze for this not real confocal question... I am recently putting mi hands on a Olympus IX81 equipped for fluorescence image acquisition and I would like to move into deconvolution of wide field images. I am asking If any of you tried this technique on this microscope, and the minimal optical/software configuration suggested (I have a light guide stable illumination, filter wheel for excitation filters, good CCD camera, vibration free environment, unfotunately not UPLANSAPO objectives at the moment) I wonder also If the Z axis motorized movement is accurate enough for the purpose, the company says 10 nm steps... Thanks, best regards Stefano Stefano Giovannardi PhD DiSTA - BRD Università dell'Insubria via J.H. Dunant 3 21100 Varese Italy [hidden email] |
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In reply to this post by Stefano Giovannardi
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** For this I can recoment SVI, a conny in The Netherlands. They know a lot of deconvolution, so please, contact them! |
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In reply to this post by Thomas Horn
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Are you referring to "First Contact"? It's a sort of polymer goo. I first saw it at Photonics West and picked up a bit to try around my lab for cleaning really dirty mirrors. It seems to work fairly well on things like grease, fingerprints and dust. It does have some glue-like properties when it dries, but doesn't have a lot of hold strength (so you can peel it off to clean the optic!) so may not work great as a glue. On the other hand, it may be just sticky enough to work as a temporary solution. If you have a large contact area I think it would hold fairly well. Do note that it doesn't play well with many plastics because of the solvents used to keep the polymer liquid for the initial application... Craig On Wed, Jun 27, 2012 at 3:29 AM, Horn Thomas <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Tobias, > A colleague showed to me a polymer that is made to clean optical surfaces. > It works like a glue: you pour it onto the surface, it polymerizes and then > you peel it off like silicon with all the dirt and dust. So it should work > temporarily like also as an adhesive to stick a mirror to another surface. > The advantage is, its made for optics so it will not harm any coatings or > delicate surface. See link below > Best regards, > Thomas > ETH > Basel > > > No commercial interest > > > http://www.photoniccleaning.com/products/#original solutions > > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Mark Cannell > Sent: Montag, 25. Juni 2012 17:45 > To: [hidden email] > Subject: Re: Removable glue for optics > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Three thoughts: > > PVA blobs which can be peeled after setting or hot glue (stronger -harder > to peel). Nail varnish is good too. I've used all 3 but YMMV depending on > area of contact etc. > > HTH > > Mark > > > On 25/06/2012, at 4:38 PM, Tobias Rose wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Dear all, > > > > I need to glue some glass optics onto anodized aluminum surfaces (edges > of coated dichroic mirror surface and of a full reflective silver mirror). > The problem is that I'd like to be able to remove the mirrors at some point > without completely ruining their reflective surfaces and leaving too much > residue on the glass and aluminum. > > The glued parts are not in the optical path so the glue does not have to > be transparent. Also the glue can be quite weak, just enough so that the > mirrors don't fall off. > > > > Can anyone suggest a nice glue for that? > > > > Best, > > Tobias > |
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In reply to this post by Guy Cox-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Thanks to everybody for their feedback. It certainely will help us in making the right decision for our needs. Regards, -Prabhakar On 6/27/2012 4:27 AM, Guy Cox wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > It is generally considered good form to put "Commercial Response" in the header of messages like this. Of course we all value messages from vendors, but it is nice to be able to distinguish them. > > Guy > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Guy Cox, MA, DPhil(Oxon), Honorary Associate, > Australian Centre for Microscopy & Microanalysis, > Madsen Building F09, University of Sydney, NSW 2006 > > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 > ______________________________________________ > http://www.guycox.net > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mark Donaghy > Sent: Wednesday, 27 June 2012 7:03 PM > To: [hidden email] > Subject: Re: CMOS Camera > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Prabhakar, > > They are indeed different chips, hence different characteristics. Andor is using > the Fairchild chip. There are other sCMOS cameras to consider as well. Raptor > offers a sCMOS camera (Osprey) which uses a CMOSIS chip (you can Google > these guys to find out more information). This is a 4.2MP (2048x2048) chip with > 64% QE at 600nm. It also uses a global shutter, something to consider. It is a > small, light camera that offers a very attractive price point. Feel free to contact > me for more information. > > Mark > |
 
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Steffen Dietzel |
'First contact' cleaning reagent. was: Removable glue for optics
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In reply to this post by Craig Brideau
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Has anybody tried this 'First contact' reagent for cleaning the front lenses of objectives? In theory, it should be perfect to clean e.g. concave lenses with narrow diameter without leaving residues. However, it contains acetone as a solvent, so I am wondering how the objective's glue is going to respond to that. Does this pose a risk or are current day objectives acetone resistant? The list of components from the MSDS: FORMAL GLYCOL 10-40% BIS(METHOXY)METHANE 10-30% ETHYL ALCOHOL 30-50% ACETONE 10-30% ETHYL LACTATE <10% ETHYL ACETATE <10% Cheers, Steffen On 27.06.2012 19:52, Craig Brideau wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Are you referring to "First Contact"? It's a sort of polymer goo. I first > saw it at Photonics West and picked up a bit to try around my lab for > cleaning really dirty mirrors. It seems to work fairly well on things like > grease, fingerprints and dust. It does have some glue-like properties when > it dries, but doesn't have a lot of hold strength (so you can peel it off > to clean the optic!) so may not work great as a glue. On the other hand, > it may be just sticky enough to work as a temporary solution. If you have > a large contact area I think it would hold fairly well. Do note that it > doesn't play well with many plastics because of the solvents used to keep > the polymer liquid for the initial application... > > Craig > > > > On Wed, Jun 27, 2012 at 3:29 AM, Horn Thomas<[hidden email]>wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Hi Tobias, >> A colleague showed to me a polymer that is made to clean optical surfaces. >> It works like a glue: you pour it onto the surface, it polymerizes and then >> you peel it off like silicon with all the dirt and dust. So it should work >> temporarily like also as an adhesive to stick a mirror to another surface. >> The advantage is, its made for optics so it will not harm any coatings or >> delicate surface. See link below >> Best regards, >> Thomas >> ETH >> Basel >> >> >> No commercial interest >> >> >> http://www.photoniccleaning.com/products/#original solutions >> >> >> >> >> >> -----Original Message----- >> From: Confocal Microscopy List [mailto:[hidden email]] >> On Behalf Of Mark Cannell >> Sent: Montag, 25. Juni 2012 17:45 >> To: [hidden email] >> Subject: Re: Removable glue for optics >> >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Three thoughts: >> >> PVA blobs which can be peeled after setting or hot glue (stronger -harder >> to peel). Nail varnish is good too. I've used all 3 but YMMV depending on >> area of contact etc. >> >> HTH >> >> Mark >> >> >> On 25/06/2012, at 4:38 PM, Tobias Rose wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> Dear all, >>> >>> I need to glue some glass optics onto anodized aluminum surfaces (edges >> of coated dichroic mirror surface and of a full reflective silver mirror). >> The problem is that I'd like to be able to remove the mirrors at some point >> without completely ruining their reflective surfaces and leaving too much >> residue on the glass and aluminum. >>> The glued parts are not in the optical path so the glue does not have to >> be transparent. Also the glue can be quite weak, just enough so that the >> mirrors don't fall off. >>> >>> Can anyone suggest a nice glue for that? >>> >>> Best, >>> Tobias >> > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Walter-Brendel-Zentrum für experimentelle Medizin (WBex) Head of light microscopy Mail room: Marchioninistr. 15, D-81377 München Building location: Marchioninistr. 27, München-Großhadern |
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Glen MacDonald-2 |
Re: 'First contact' cleaning reagent. was: Removable glue for optics
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Treat it like any other solvent. The product states very clearly to avoid contact with plastics and paint unless without carefully testing on an expendable subject. It requires about 20 min to dry which would hold the solvent in contact with any lens glue situated beneath the plastic layer. On the other hand, some lenses appear to have a relatively wide margin of glass between the polished region and the mountant. In those cases maybe it could be applied carefully to avoid the glue. But, who knows how the coating will behave? I found that the plastic back apertures of newer Zeiss objectives will dissolve in methanol! Glen Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 [hidden email] On Jun 28, 2012, at 1:52 AM, Steffen Dietzel wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Has anybody tried this 'First contact' reagent for cleaning the front lenses of objectives? In theory, it should be perfect to clean e.g. concave lenses with narrow diameter without leaving residues. > > However, it contains acetone as a solvent, so I am wondering how the objective's glue is going to respond to that. Does this pose a risk or are current day objectives acetone resistant? The list of components from the MSDS: > FORMAL GLYCOL 10-40% > BIS(METHOXY)METHANE 10-30% > ETHYL ALCOHOL 30-50% > ACETONE 10-30% > ETHYL LACTATE <10% > ETHYL ACETATE <10% > > Cheers, > Steffen > > > On 27.06.2012 19:52, Craig Brideau wrote: >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Are you referring to "First Contact"? It's a sort of polymer goo. I first >> saw it at Photonics West and picked up a bit to try around my lab for >> cleaning really dirty mirrors. It seems to work fairly well on things like >> grease, fingerprints and dust. It does have some glue-like properties when >> it dries, but doesn't have a lot of hold strength (so you can peel it off >> to clean the optic!) so may not work great as a glue. On the other hand, >> it may be just sticky enough to work as a temporary solution. If you have >> a large contact area I think it would hold fairly well. Do note that it >> doesn't play well with many plastics because of the solvents used to keep >> the polymer liquid for the initial application... >> >> Craig >> >> >> >> On Wed, Jun 27, 2012 at 3:29 AM, Horn Thomas<[hidden email]>wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> Hi Tobias, >>> A colleague showed to me a polymer that is made to clean optical surfaces. >>> It works like a glue: you pour it onto the surface, it polymerizes and then >>> you peel it off like silicon with all the dirt and dust. So it should work >>> temporarily like also as an adhesive to stick a mirror to another surface. >>> The advantage is, its made for optics so it will not harm any coatings or >>> delicate surface. See link below >>> Best regards, >>> Thomas >>> ETH >>> Basel >>> >>> >>> No commercial interest >>> >>> >>> http://www.photoniccleaning.com/products/#original solutions >>> >>> >>> >>> >>> >>> -----Original Message----- >>> From: Confocal Microscopy List [mailto:[hidden email]] >>> On Behalf Of Mark Cannell >>> Sent: Montag, 25. Juni 2012 17:45 >>> To: [hidden email] >>> Subject: Re: Removable glue for optics >>> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> Three thoughts: >>> >>> PVA blobs which can be peeled after setting or hot glue (stronger -harder >>> to peel). Nail varnish is good too. I've used all 3 but YMMV depending on >>> area of contact etc. >>> >>> HTH >>> >>> Mark >>> >>> >>> On 25/06/2012, at 4:38 PM, Tobias Rose wrote: >>> >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> ***** >>>> >>>> Dear all, >>>> >>>> I need to glue some glass optics onto anodized aluminum surfaces (edges >>> of coated dichroic mirror surface and of a full reflective silver mirror). >>> The problem is that I'd like to be able to remove the mirrors at some point >>> without completely ruining their reflective surfaces and leaving too much >>> residue on the glass and aluminum. >>>> The glued parts are not in the optical path so the glue does not have to >>> be transparent. Also the glue can be quite weak, just enough so that the >>> mirrors don't fall off. >>>> >>>> Can anyone suggest a nice glue for that? >>>> >>>> Best, >>>> Tobias >>> >> > > > -- > ------------------------------------------------------------ > Steffen Dietzel, PD Dr. rer. nat > Ludwig-Maximilians-Universität München > Walter-Brendel-Zentrum für experimentelle Medizin (WBex) > Head of light microscopy > > Mail room: > Marchioninistr. 15, D-81377 München > > Building location: > Marchioninistr. 27, München-Großhadern |
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Craig Brideau |
Re: 'First contact' cleaning reagent. was: Removable glue for optics
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Your news that the back apertures of Zeiss objectives dissolves in methanol was quite alarming! Thanks for sharing that warning. Regarding the First Contact stuff; I make a point of never letting it touch anything other than glass. If I have to clean an optic, I only use it on the center. I am careful to stay away from the edges of any mount just in case the solvents damage things. Craig On Thu, Jun 28, 2012 at 8:58 AM, Glen MacDonald <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Treat it like any other solvent. The product states very clearly to avoid > contact with plastics and paint unless without carefully testing on an > expendable subject. It requires about 20 min to dry which would hold the > solvent in contact with any lens glue situated beneath the plastic layer. > On the other hand, some lenses appear to have a relatively wide margin of > glass between the polished region and the mountant. In those cases maybe > it could be applied carefully to avoid the glue. But, who knows how the > coating will behave? I found that the plastic back apertures of newer Zeiss > objectives will dissolve in methanol! > > Glen > > Glen MacDonald > Core for Communication Research > Virginia Merrill Bloedel Hearing Research Center > Box 357923 > University of Washington > Seattle, WA 98195-7923 USA > (206) 616-4156 > [hidden email] > > > > > On Jun 28, 2012, at 1:52 AM, Steffen Dietzel wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Has anybody tried this 'First contact' reagent for cleaning the front > lenses of objectives? In theory, it should be perfect to clean e.g. concave > lenses with narrow diameter without leaving residues. > > > > However, it contains acetone as a solvent, so I am wondering how the > objective's glue is going to respond to that. Does this pose a risk or are > current day objectives acetone resistant? The list of components from the > MSDS: > > FORMAL GLYCOL 10-40% > > BIS(METHOXY)METHANE 10-30% > > ETHYL ALCOHOL 30-50% > > ACETONE 10-30% > > ETHYL LACTATE <10% > > ETHYL ACETATE <10% > > > > Cheers, > > Steffen > > > > > > On 27.06.2012 19:52, Craig Brideau wrote: > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> ***** > >> > >> Are you referring to "First Contact"? It's a sort of polymer goo. I > first > >> saw it at Photonics West and picked up a bit to try around my lab for > >> cleaning really dirty mirrors. It seems to work fairly well on things > like > >> grease, fingerprints and dust. It does have some glue-like properties > when > >> it dries, but doesn't have a lot of hold strength (so you can peel it > off > >> to clean the optic!) so may not work great as a glue. On the other > hand, > >> it may be just sticky enough to work as a temporary solution. If you > have > >> a large contact area I think it would hold fairly well. Do note that it > >> doesn't play well with many plastics because of the solvents used to > keep > >> the polymer liquid for the initial application... > >> > >> Craig > >> > >> > >> > >> On Wed, Jun 27, 2012 at 3:29 AM, Horn Thomas<[hidden email] > >wrote: > >> > >>> ***** > >>> To join, leave or search the confocal microscopy listserv, go to: > >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>> ***** > >>> > >>> Hi Tobias, > >>> A colleague showed to me a polymer that is made to clean optical > surfaces. > >>> It works like a glue: you pour it onto the surface, it polymerizes and > then > >>> you peel it off like silicon with all the dirt and dust. So it should > work > >>> temporarily like also as an adhesive to stick a mirror to another > surface. > >>> The advantage is, its made for optics so it will not harm any coatings > or > >>> delicate surface. See link below > >>> Best regards, > >>> Thomas > >>> ETH > >>> Basel > >>> > >>> > >>> No commercial interest > >>> > >>> > >>> http://www.photoniccleaning.com/products/#original solutions > >>> > >>> > >>> > >>> > >>> > >>> -----Original Message----- > >>> From: Confocal Microscopy List [mailto: > [hidden email]] > >>> On Behalf Of Mark Cannell > >>> Sent: Montag, 25. Juni 2012 17:45 > >>> To: [hidden email] > >>> Subject: Re: Removable glue for optics > >>> > >>> ***** > >>> To join, leave or search the confocal microscopy listserv, go to: > >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>> ***** > >>> > >>> Three thoughts: > >>> > >>> PVA blobs which can be peeled after setting or hot glue (stronger > -harder > >>> to peel). Nail varnish is good too. I've used all 3 but YMMV > depending on > >>> area of contact etc. > >>> > >>> HTH > >>> > >>> Mark > >>> > >>> > >>> On 25/06/2012, at 4:38 PM, Tobias Rose wrote: > >>> > >>>> ***** > >>>> To join, leave or search the confocal microscopy listserv, go to: > >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>>> ***** > >>>> > >>>> Dear all, > >>>> > >>>> I need to glue some glass optics onto anodized aluminum surfaces > (edges > >>> of coated dichroic mirror surface and of a full reflective silver > mirror). > >>> The problem is that I'd like to be able to remove the mirrors at some > point > >>> without completely ruining their reflective surfaces and leaving too > much > >>> residue on the glass and aluminum. > >>>> The glued parts are not in the optical path so the glue does not have > to > >>> be transparent. Also the glue can be quite weak, just enough so that > the > >>> mirrors don't fall off. > >>>> > >>>> Can anyone suggest a nice glue for that? > >>>> > >>>> Best, > >>>> Tobias > >>> > >> > > > > > > -- > > ------------------------------------------------------------ > > Steffen Dietzel, PD Dr. rer. nat > > Ludwig-Maximilians-Universität München > > Walter-Brendel-Zentrum für experimentelle Medizin (WBex) > > Head of light microscopy > > > > Mail room: > > Marchioninistr. 15, D-81377 München > > > > Building location: > > Marchioninistr. 27, München-Großhadern > |
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David Baddeley |
Re: 'First contact' cleaning reagent. was: Removable glue for optics
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In reply to this post by Steffen Dietzel
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I've seen the consequences of acetone on a modern objective front lens first hand, and it ain't pretty (the first element of the lens started to 'float' and could be moved independently of the rest of the objective by pushing on it with a cotton bud). Suffice to say it was a very expensive repair, and I'd avoid solvents anywhere near the front lens. cheers, David ________________________________ From: Steffen Dietzel <[hidden email]> To: [hidden email] Sent: Thursday, 28 June 2012 8:52 PM Subject: 'First contact' cleaning reagent. was: Removable glue for optics ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Has anybody tried this 'First contact' reagent for cleaning the front lenses of objectives? In theory, it should be perfect to clean e.g. concave lenses with narrow diameter without leaving residues. However, it contains acetone as a solvent, so I am wondering how the objective's glue is going to respond to that. Does this pose a risk or are current day objectives acetone resistant? The list of components from the MSDS: FORMAL GLYCOL 10-40% BIS(METHOXY)METHANE 10-30% ETHYL ALCOHOL 30-50% ACETONE 10-30% ETHYL LACTATE <10% ETHYL ACETATE <10% Cheers, Steffen On 27.06.2012 19:52, Craig Brideau wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Are you referring to "First Contact"? It's a sort of polymer goo. I first > saw it at Photonics West and picked up a bit to try around my lab for > cleaning really dirty mirrors. It seems to work fairly well on things like > grease, fingerprints and dust. It does have some glue-like properties when > it dries, but doesn't have a lot of hold strength (so you can peel it off > to clean the optic!) so may not work great as a glue. On the other hand, > it may be just sticky enough to work as a temporary solution. If you have > a large contact area I think it would hold fairly well. Do note that it > doesn't play well with many plastics because of the solvents used to keep > the polymer liquid for the initial application... > > Craig > > > > On Wed, Jun 27, 2012 at 3:29 AM, Horn Thomas<[hidden email]>wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Hi Tobias, >> A colleague showed to me a polymer that is made to clean optical surfaces. >> It works like a glue: you pour it onto the surface, it polymerizes and then >> you peel it off like silicon with all the dirt and dust. So it should work >> temporarily like also as an adhesive to stick a mirror to another surface. >> The advantage is, its made for optics so it will not harm any coatings or >> delicate surface. See link below >> Best regards, >> Thomas >> ETH >> Basel >> >> >> No commercial interest >> >> >> http://www.photoniccleaning.com/products/#original solutions >> >> >> >> >> >> -----Original Message----- >> From: Confocal Microscopy List [mailto:[hidden email]] >> On Behalf Of Mark Cannell >> Sent: Montag, 25. Juni 2012 17:45 >> To: [hidden email] >> Subject: Re: Removable glue for optics >> >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Three thoughts: >> >> PVA blobs which can be peeled after setting or hot glue (stronger -harder >> to peel). Nail varnish is good too. I've used all 3 but YMMV depending on >> area of contact etc. >> >> HTH >> >> Mark >> >> >> On 25/06/2012, at 4:38 PM, Tobias Rose wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> Dear all, >>> >>> I need to glue some glass optics onto anodized aluminum surfaces (edges >> of coated dichroic mirror surface and of a full reflective silver mirror). >> The problem is that I'd like to be able to remove the mirrors at some point >> without completely ruining their reflective surfaces and leaving too much >> residue on the glass and aluminum. >>> The glued parts are not in the optical path so the glue does not have to >> be transparent. Also the glue can be quite weak, just enough so that the >> mirrors don't fall off. >>> >>> Can anyone suggest a nice glue for that? >>> >>> Best, >>> Tobias >> > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Walter-Brendel-Zentrum für experimentelle Medizin (WBex) Head of light microscopy Mail room: Marchioninistr. 15, D-81377 München Building location: Marchioninistr. 27, München-Großhadern |
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