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Request: Hamamatsu Orca ER manual...

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Peter Gabriel Pitrone

Request: Hamamatsu Orca ER manual...

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Howdy Y'all,

This is not a confocal related question. BUT since this forum is such a
big group of microscopists, I'd figure I would try anyway...

I am currently trying to find a PDF version of the Hamamatsu Orca ER user
guide/manual. Does anyone have access to it?! Searches on Google only
brought up the datasheet.

Thanks for any and all help in advance!!

Regards,
Pete

--
Peter Gabriel Pitrone - TechRMS
Microscopy/Imaging Specialist
Prof. Dr. Pavel Tomancak group
Max Planck Institute for
Molecular Biology and Genetics
Pfotenhauerstr. 108
01307 Dresden

"If a straight line fit is required, obtain only two data points." - Anon.

Tobias Rose

Re: Request: Hamamatsu Orca ER manual...

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...I know. Finding Hamamatsu manuals is a pain! I managed to get my hands on the Orca05G02 manual.
This camera has a 150ER CCD - not sure if that will help you.

http://goo.gl/n6egt

cheers,
Tobias

> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]] On Behalf Of Peter
> Gabriel Pitrone
> Sent: Monday, November 26, 2012 12:30 PM
> To: [hidden email]
> Subject: Request: Hamamatsu Orca ER manual...
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Howdy Y'all,
>
> This is not a confocal related question. BUT since this forum is such a big
> group of microscopists, I'd figure I would try anyway...
>
> I am currently trying to find a PDF version of the Hamamatsu Orca ER user
> guide/manual. Does anyone have access to it?! Searches on Google only
> brought up the datasheet.
>
> Thanks for any and all help in advance!!
>
> Regards,
> Pete
>
> --
> Peter Gabriel Pitrone - TechRMS
> Microscopy/Imaging Specialist
> Prof. Dr. Pavel Tomancak group
> Max Planck Institute for
> Molecular Biology and Genetics
> Pfotenhauerstr. 108
> 01307 Dresden
>
> "If a straight line fit is required, obtain only two data points." - Anon.

Peter Gabriel Pitrone

Re: Request: Hamamatsu Orca ER manual...

Peter Gabriel Pitrone

Re: Request: Hamamatsu Orca ER manual...

In reply to this post by Peter Gabriel Pitrone

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To all it may concern,

I got a copy from Hamamatsu, so I don't need anymore help finding it.
Thanks to everyone who replied and were willing to help...

Pete

On Mon, November 26, 2012 12:30 pm, Peter Gabriel Pitrone wrote:
| *****
| To join, leave or search the confocal microscopy listserv, go to:
| http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
| *****
|
| Howdy Y'all,
|
| This is not a confocal related question. BUT since this forum is such a
| big group of microscopists, I'd figure I would try anyway...
|
| I am currently trying to find a PDF version of the Hamamatsu Orca ER user
| guide/manual. Does anyone have access to it?! Searches on Google only
| brought up the datasheet.
|
| Thanks for any and all help in advance!!
|
| Regards,
| Pete
|
| --
| Peter Gabriel Pitrone - TechRMS
| Microscopy/Imaging Specialist
| Prof. Dr. Pavel Tomancak group
| Max Planck Institute for
| Molecular Biology and Genetics
| Pfotenhauerstr. 108
| 01307 Dresden
|
| "If a straight line fit is required, obtain only two data points." - Anon.
|

--
Peter Gabriel Pitrone - TechRMS
Microscopy/Imaging Specialist
Prof. Dr. Pavel Tomancak group
Max Planck Institute for
Molecular Biology and Genetics
Pfotenhauerstr. 108
01307 Dresden

"If a straight line fit is required, obtain only two data points." - Anon.

Vitaly Boyko

8 um/pixel EMCCD

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Dear All,

We are looking for an 1024x1024 EMCCD camera with smaller pixels than 13-16 um. Though I do not follow in great detail recent developments in EMCCD technology, there are probably no 1024x1024 EMCCD cameras with 16um/pixel.
I would appreciate your feedback on 8 um/pixel EMCCDs for wide-field/TIRF microscopy. I assume that new CMOS cameras have little advantage over interline CCDs alike Orca AG, except for profit making that is of interest to vendors only.

Are there any EMCCD cameras with 6.4 um/pixel?

Many thanks in advance,

Vitaly

lechristophe

Re: 8 um/pixel EMCCD

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Dear Vitaly,

As regards sensors, e2V has a 16µM 1024x1024 sensor that is used in
the Andor Ixon 888. Texas Instruments has a 8 µM 1002x1004 EMCCD
sensor that was used in the first Hamamatsu EMCCD camera but the QE
was quite low as it was not back-illuminated. See this table with all
existing sensors (maybe new ones are coming):

http://www.emccd.com/what_is_emccd/emccd_sensors/

Cheers,

Christophe

On Wed, Nov 28, 2012 at 8:52 PM, Vitaly Boyko <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All,
>
> We are looking for an 1024x1024 EMCCD camera with smaller pixels than 13-16 um. Though I do not follow in great detail recent developments in EMCCD technology, there are probably no 1024x1024 EMCCD cameras with 16um/pixel.
> I would appreciate your feedback on 8 um/pixel EMCCDs for wide-field/TIRF microscopy. I assume that new CMOS cameras have little advantage over interline CCDs alike Orca AG, except for profit making that is of interest to vendors only.
>
> Are there any EMCCD cameras with 6.4 um/pixel?
>
> Many thanks in advance,
>
> Vitaly

Csúcs Gábor

Re: 8 um/pixel EMCCD

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Dear Vitaly,

Christophe summarized very nicely the details/available information about
EMCCDs. I'd add just one remark: I kind of disagree with you about the
performance of the new sCMOS cameras. I our hands (we tested the Andor Neo
and the Hamamatsu Flash 4) they clearly outperformed the "standard"
interline CCDs so we have decided gradually upgrade all of our wide-field
systems with those cameras. Furthermore, (of course this is very much
company dependent) they are not so much more expensive then an interline
camera (if bought new). There is some (clearly somewhat debated) data
available about the sCMOS cameras even out-performing the back-illuminated
EMCCDs. This is certainly not true for all applications and I don't want
to into the subject, but overall I think that sCMOS cameras are a very
interesting choice/alternative for fluorescent imaging.

Greetings Gabor

Light Microscopy and Screening Centre
ETH Zurich, HPM G41
Schafmattstrasse 18
CH-8093, Zurich
Switzerland

Phone: +41 44 633 6221
Mobile: +41 79 7582158

On 11/29/12 9:50 AM, "Christophe Leterrier"
<[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Dear Vitaly,
>
>As regards sensors, e2V has a 16µM 1024x1024 sensor that is used in
>the Andor Ixon 888. Texas Instruments has a 8 µM 1002x1004 EMCCD
>sensor that was used in the first Hamamatsu EMCCD camera but the QE
>was quite low as it was not back-illuminated. See this table with all
>existing sensors (maybe new ones are coming):
>
>http://www.emccd.com/what_is_emccd/emccd_sensors/
>
>Cheers,
>
>Christophe
>
>On Wed, Nov 28, 2012 at 8:52 PM, Vitaly Boyko <[hidden email]>
>wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear All,
>>
>> We are looking for an 1024x1024 EMCCD camera with smaller pixels than
>>13-16 um. Though I do not follow in great detail recent developments in
>>EMCCD technology, there are probably no 1024x1024 EMCCD cameras with
>>16um/pixel.
>> I would appreciate your feedback on 8 um/pixel EMCCDs for
>>wide-field/TIRF microscopy. I assume that new CMOS cameras have little
>>advantage over interline CCDs alike Orca AG, except for profit making
>>that is of interest to vendors only.
>>
>> Are there any EMCCD cameras with 6.4 um/pixel?
>>
>> Many thanks in advance,
>>
>> Vitaly

Orla Hanrahan

Re: 8 um/pixel EMCCD

In reply to this post by Vitaly Boyko

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Dear Vitaly,

Andor have an iXon 888 EMCCD camera which has a pixel format of 1024 x 1024
and 13 um pixels. In addition, we have the Luca R EMCCD which has a pixel
format of 1000 x 1000 and a pixel size of 8 um. For more detailed
information on these EMCCD cameras please refer to the specifications on our
website:

http://www.andor.com/scientific-cameras/ixon-emccd-camera-series/ixon3-888
http://www.andor.com/scientific-cameras/luca-emccd-camera-series/luca-r

If you would like to discuss these options further please contact me.

Best wishes,

Orla

Andriy Chmyrov

Re: 8 um/pixel EMCCD

In reply to this post by Vitaly Boyko

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Dear Vitaly,

Andor has following cameras:
Luca R, 1004 x 1002 pixels, cooling to -20C, 8 x 8 µm pixels, 12.4 fps
Luca S, 658 x 496 pixels, cooling to -20, 10 x 10 µm pixels, 37.2 fps

I had very positive impressions from working with Luca S camera, and it's
cost efficient.

Best,
Andriy

James Joubert

Re: 8 um/pixel EMCCD

In reply to this post by Vitaly Boyko

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Dear Vitaly,

You are right that there are no current 1024x1024 16um pixel EMCCD cameras
on the market. There is a TI EMCCD chip with 1004x1002 array and 8um pixels,
front illuminated. There is also an e2v EMCCD chip with 1024x1024 array,
13um pixels, and back illuminated. These might serve your needs.
The TI chip is available in QImaging's EM-C2, which is remarkably fast:
http://www.qimaging.com/products/cameras/scientific/rolera-emc2.php
The e2v chip is available in the Photometrics CascadeII:1024 and the
Princeton Instruments ProEM+:1024B:
http://www.photometrics.com/products/emccdcams/cascade2_1024.php
http://www.princetoninstruments.com/products/imcam/proem/

Since you mentioned CMOS, you may wish to check out a 4-part series in
Microscopy & Analysis discussing camera fundamentals and comparing CMOS,
EMCCD, and CCD technologies. I contributed some material, and it's an
interesting read. You can find it here:

http://www.microscopy-analysis.com/magazine-article/digital-camera-technologies-scientific-bio-imaging-part-1-sensors

If you have any further questions, please shoot me an email.

Best regards,

James

Vitaly Boyko

Re: 8 um/pixel EMCCD

In reply to this post by Orla Hanrahan

Thank you very much for the update. I am very much surprised
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Dear All,

Thank you very much for the update. I am very much surprised if not shocked that for the last five years there was no progress along 8 um EMCCDs, and of non-existence of 8um/pixel BI-EMCCDs. sCMOS cameras seem to represent a good alternative to interline CCDs, especially the cooled ones with 4-5.5 MPixels.

With best regards,

Vitaly

________________________________
From: Orla Hanrahan <[hidden email]>
To: [hidden email]
Sent: Thursday, November 29, 2012 7:04 AM
Subject: Re: 8 um/pixel EMCCD

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Dear Vitaly,

Andor have an iXon 888 EMCCD camera which has a pixel format of 1024 x 1024
and 13 um pixels. In addition, we have the Luca R EMCCD which has a pixel
format of 1000 x 1000 and a pixel size of 8 um. For more detailed
information on these EMCCD cameras please refer to the specifications on our
website:

http://www.andor.com/scientific-cameras/ixon-emccd-camera-series/ixon3-888
http://www.andor.com/scientific-cameras/luca-emccd-camera-series/luca-r

If you would like to discuss these options further please contact me.

Best wishes,

Orla