Request for a working protocol

[sunandod]

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Hello Everyone
    We are planning to label fribronectin(Sigma) with Alexa 647. Our
starting amount is less than 5 micro gram of Fibronectin.
Do anyone of you have a working protocol to follow?

Thanks in advance.
Sunando

[mcfrain]

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Hi Sunando,

It depends on what assay you are performing, although our lab published a
paper on Molecular and Cellular Biology where we applied a protocol to
monitor the FN remodeling. You can find the paper through:
Galectin Binding to Mgat5-Modified N-Glycans Regulates Fibronectin Matrix
Remodeling in Tumor Cells <http://mcb.asm.org/content/26/8/3181.full>

Mol Cell Biol.<http://www.ncbi.nlm.nih.gov/pubmed/?term=Galectin+Binding+to+Mgat5-Modified+N-Glycans+Regulates+Fibronectin+Matrix+Remodeling+in+Tumor+Cells#>2006
Apr;26(8):3181-93.
Lagana A<http://www.ncbi.nlm.nih.gov/pubmed?term=Lagana%20A%5BAuthor%5D&cauthor=true&cauthor_uid=16581792>,
Goetz JG<http://www.ncbi.nlm.nih.gov/pubmed?term=Goetz%20JG%5BAuthor%5D&cauthor=true&cauthor_uid=16581792>,
Cheung P<http://www.ncbi.nlm.nih.gov/pubmed?term=Cheung%20P%5BAuthor%5D&cauthor=true&cauthor_uid=16581792>,
Raz A<http://www.ncbi.nlm.nih.gov/pubmed?term=Raz%20A%5BAuthor%5D&cauthor=true&cauthor_uid=16581792>,
Dennis JW<http://www.ncbi.nlm.nih.gov/pubmed?term=Dennis%20JW%5BAuthor%5D&cauthor=true&cauthor_uid=16581792>,
Nabi IR<http://www.ncbi.nlm.nih.gov/pubmed?term=Nabi%20IR%5BAuthor%5D&cauthor=true&cauthor_uid=16581792>
.


Cheers,

Ray


On Mon, Aug 12, 2013 at 10:10 AM, sunando_imap <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> Hello Everyone
>    We are planning to label fribronectin(Sigma) with Alexa 647. Our
> starting amount is less than 5 micro gram of Fibronectin.
> Do anyone of you have a working protocol to follow?
>
> Thanks in advance.
> Sunando
>