Resonant scanner from A1R vs SP5
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Dear list,
I'm looking for general information about the comparison between the resonant scanners in the SP5 versus A1R. Apart other comments, are possible future upgrades by software in the image format, panning and quick switching between the high resolution scanner and the resonant one? Thank you very much in advance. -- Juan Luis Ribas Servicio de Microscopía Centro de Investigación, Tecnología e Innovación Universidad de Sevilla Av. Reina Mercedes 4b 41012 Sevilla |
 
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George McNamara |
Re: Resonant scanner from A1R vs SP5 ...
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Hi Juan,
On the SP5, when you start LAS AF the choice is resonant scanner or high resolution scanner. No way currently to quickly switch, but do you really need to? If you have a motorized stage, you can tile scan in resonant scanner mode faster than you could exit and restart. George p.s. I've noticed on both the Leica SP5, Zeiss LSM510 and Zeiss LSM710 that "faster is brighter" for many fluorophores. That is, a 0.4 us dwell time results in a brighter image than a 4 us which is brighter than a 40 us. This implies that <0.4 us might be even brighter - i.e. resonant scanner mode. A couple of possible explanations (not mutually exclusive):
high speed scanning has the potential to increase fluorescence yield and to reduce photobleaching. Borlinghaus RT. Microsc Res Tech. 2006 Sep;69(9):689-92.PMID: 16878313 At 12:37 PM 5/7/2010, you wrote: Dear list, George McNamara, Ph.D. Image Core Manager Analytical Imaging Core Facility University of Miami, Miller School of Medicine Miami, FL 33136 [hidden email] [hidden email] 305-243-8436 office http://www.sylvester.org/AICF (Analytical Imaging Core Facility) http://www.sylvester.org/AICF/pubspectra.zip (the entire 2000+ spectra .xlsx file is in the zip file) http://home.earthlink.net/~geomcnamara |
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Maclyn McCarty |
Re: Resonant scanner from A1R vs SP5 ...
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Hi List,
Is anyone out there using the eLabExperts booking, charging etc system? see http://www.elabexperts.com/core/default.aspx it looks really good and seems to be a more refined (and payed for) version of PPMS If you have it, what has your experience been? Does it do everything it should? Cheers Cam *Cameron J. Nowell *Microscopy Manager Centre for Advanced Microscopy Ludwig Institute for Cancer Research PO Box 2008 Royal Melbourne Hospital Victoria, 3050 AUSTRALIA *Office*: +61 3 9341 3155 *Mobile*: +61422882700 *Fax*: +61 3 9341 3104 Facility Website <http://www.ludwig.edu.au/confocal/> -----Original Message----- From: Confocal Microscopy List on behalf of Maclyn McCarty Sent: Mon 24/05/2010 11:26 PM To: [hidden email] Subject: Re: Resonant scanner from A1R vs SP5 ... What is the advantage of using resonant scanner? Is there any technical comparison of the resonant scanner of A1 and SP5? Mac. On Sat, 8/5/10, George McNamara <[hidden email]> wrote: From: George McNamara <[hidden email]> Subject: Re: Resonant scanner from A1R vs SP5 ... To: [hidden email] Date: Saturday, 8 May, 2010, 2:45 PM Hi Juan, On the SP5, when you start LAS AF the choice is resonant scanner or high resolution scanner. No way currently to quickly switch, but do you really need to? If you have a motorized stage, you can tile scan in resonant scanner mode faster than you could exit and restart. George p.s. I've noticed on both the Leica SP5, Zeiss LSM510 and Zeiss LSM710 that "faster is brighter" for many fluorophores. That is, a 0.4 us dwell time results in a brighter image than a 4 us which is brighter than a 40 us. This implies that <0.4 us might be even brighter - i.e. resonant scanner mode. A couple of possible explanations (not mutually exclusive): a) photophysics - re TRex (PubMed 19337661), papers by Sanden/Spielmann/Widergren et al (PubMed 20375039, 20196585, 19007245, 17385841). b) calibration method(s) by Zeiss and/or Leica that try to (but do not always) match output at all settings. See also high speed scanning has the potential to increase fluorescence yield and to reduce photobleaching. Borlinghaus RT. Microsc Res Tech. 2006 Sep;69(9):689-92.PMID: 16878313 At 12:37 PM 5/7/2010, you wrote: Dear list, I'm looking for general information about the comparison between the resonant scanners in the SP5 versus A1R. Apart other comments, are possible future upgrades by software in the image format, panning and quick switching between the high resolution scanner and the resonant one? Thank you very much in advance. -- Juan Luis Ribas Servicio de Microscopía Centro de Investigación, Tecnología e Innovación Universidad de Sevilla Av. Reina Mercedes 4b 41012 Sevilla |
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yuansheng sun |
Re: eLabExperts Management System
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We just started using it. I think it is a very good and useful tool
for managing a facility. It meets all our needs for managing our facility. Cheers. - sheng Yuansheng Sun, Ph.D Keck Center for Cellular Imaging University of Virginia On Tue, May 25, 2010 at 6:59 AM, Cameron Nowell <[hidden email]> wrote: > Hi List, > > Is anyone out there using the eLabExperts booking, charging etc system? see http://www.elabexperts.com/core/default.aspx > > it looks really good and seems to be a more refined (and payed for) version of PPMS > > If you have it, what has your experience been? Does it do everything it should? > > > Cheers > > > Cam > > > > *Cameron J. Nowell > *Microscopy Manager > Centre for Advanced Microscopy > Ludwig Institute for Cancer Research > PO Box 2008 > Royal Melbourne Hospital > Victoria, 3050 > AUSTRALIA > > *Office*: +61 3 9341 3155 > *Mobile*: +61422882700 > *Fax*: +61 3 9341 3104 > > Facility Website <http://www.ludwig.edu.au/confocal/> > > > > > > -----Original Message----- > From: Confocal Microscopy List on behalf of Maclyn McCarty > Sent: Mon 24/05/2010 11:26 PM > To: [hidden email] > Subject: Re: Resonant scanner from A1R vs SP5 ... > > > What is the advantage of using resonant scanner? > > Is there any technical comparison of the resonant scanner of A1 and SP5? > > Mac. > > > On Sat, 8/5/10, George McNamara <[hidden email]> wrote: > > From: George McNamara <[hidden email]> > Subject: Re: Resonant scanner from A1R vs SP5 ... > To: [hidden email] > Date: Saturday, 8 May, 2010, 2:45 PM > > > > Hi Juan, > > > On the SP5, when you start LAS AF the choice is resonant scanner or high > resolution scanner. No way currently to quickly switch, but do you really > need to? If you have a motorized stage, you can tile scan in resonant > scanner mode faster than you could exit and restart. > > > George > > p.s. I've noticed on both the Leica SP5, Zeiss LSM510 and Zeiss LSM710 > that "faster is brighter" for many fluorophores. That is, a 0.4 > us dwell time results in a brighter image than a 4 us which is brighter > than a 40 us. This implies that <0.4 us might be even brighter - i.e. > resonant scanner mode. A couple of possible explanations (not mutually > exclusive): > > a) photophysics - re TRex (PubMed 19337661), papers by > Sanden/Spielmann/Widergren et al (PubMed 20375039, 20196585, 19007245, > 17385841). > b) calibration method(s) by Zeiss and/or Leica that try to (but do > not always) match output at all settings. > See also > > > high speed > scanning has the potential to increase fluorescence yield and to reduce > photobleaching. Borlinghaus RT. Microsc Res Tech. 2006 > Sep;69(9):689-92.PMID: 16878313 > > > > > > > > > At 12:37 PM 5/7/2010, you wrote: > > Dear list, > > I'm looking for general information about the comparison between the > resonant scanners in the SP5 versus A1R. Apart other comments, are > possible future upgrades by software in the image format, panning and > quick switching between the high resolution scanner and the resonant > one? > > > Thank you very much in advance. > > > -- > > > Juan Luis Ribas > > Servicio de Microscopía > > Centro de Investigación, Tecnología e Innovación > > Universidad de Sevilla > > Av. Reina Mercedes 4b > > 41012 Sevilla > > > |
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Nuno Moreno |
Re: eLabExperts Management System
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Hi!
Check our open source solution. It does everything this one does that is really useful for a facility. an example: http://calendar.igc.gulbenkian.pt/weekview.php?resource=12 -yellow entries: pre-reservations -green entries: with confirmation in situ at a certain time (entry time +/- configurable interval ). -blue entries: have waiting list with SMS or email warning in case of entry deleted -red entries: user did not show up -4 different types of resource handling: a) direct booking b) pre-booking with confirmation in situ and during entry time +/- configurable interval c) pre-booking with admin confirmation. Automatic warning for all situations with .ics integration d) just monitoring, eg, -80, ultracentrifuges, etc -Fully configurable reports with spread sheet export -different levels of administration (resource, user, permissions, etc) -harware monitoring integrated, tested on several confocals, computers, -80c freezers, Facscan, Facscalibur (temperature and pressure included), etc with sms or email warning in case of exceeding threshold If you are not a fan of open source software, we have protocols with companies to set servers and install the hardware monitoring. All the best, NM PS1: I'm starting to make the documentation. Wish me good luck :( PS2: Next week we should have a demo site. If interested please email me On 5/25/10 2:38 PM, yuansheng sun wrote: > We just started using it. I think it is a very good and useful tool > for managing a facility. It meets all our needs for managing our > facility. Cheers. - sheng > > > Yuansheng Sun, Ph.D > Keck Center for Cellular Imaging > University of Virginia > > > On Tue, May 25, 2010 at 6:59 AM, Cameron Nowell > <[hidden email]> wrote: >> Hi List, >> >> Is anyone out there using the eLabExperts booking, charging etc system? see http://www.elabexperts.com/core/default.aspx >> >> it looks really good and seems to be a more refined (and payed for) version of PPMS >> >> If you have it, what has your experience been? Does it do everything it should? >> >> >> Cheers >> >> >> Cam >> >> >> >> *Cameron J. Nowell >> *Microscopy Manager >> Centre for Advanced Microscopy >> Ludwig Institute for Cancer Research >> PO Box 2008 >> Royal Melbourne Hospital >> Victoria, 3050 >> AUSTRALIA >> >> *Office*: +61 3 9341 3155 >> *Mobile*: +61422882700 >> *Fax*: +61 3 9341 3104 >> >> Facility Website<http://www.ludwig.edu.au/confocal/> >> >> >> >> >> >> -----Original Message----- >> From: Confocal Microscopy List on behalf of Maclyn McCarty >> Sent: Mon 24/05/2010 11:26 PM >> To: [hidden email] >> Subject: Re: Resonant scanner from A1R vs SP5 ... >> >> >> What is the advantage of using resonant scanner? >> >> Is there any technical comparison of the resonant scanner of A1 and SP5? >> >> Mac. >> >> >> On Sat, 8/5/10, George McNamara<[hidden email]> wrote: >> >> From: George McNamara<[hidden email]> >> Subject: Re: Resonant scanner from A1R vs SP5 ... >> To: [hidden email] >> Date: Saturday, 8 May, 2010, 2:45 PM >> >> >> >> Hi Juan, >> >> >> On the SP5, when you start LAS AF the choice is resonant scanner or high >> resolution scanner. No way currently to quickly switch, but do you really >> need to? If you have a motorized stage, you can tile scan in resonant >> scanner mode faster than you could exit and restart. >> >> >> George >> >> p.s. I've noticed on both the Leica SP5, Zeiss LSM510 and Zeiss LSM710 >> that "faster is brighter" for many fluorophores. That is, a 0.4 >> us dwell time results in a brighter image than a 4 us which is brighter >> than a 40 us. This implies that<0.4 us might be even brighter - i.e. >> resonant scanner mode. A couple of possible explanations (not mutually >> exclusive): >> >> a) photophysics - re TRex (PubMed 19337661), papers by >> Sanden/Spielmann/Widergren et al (PubMed 20375039, 20196585, 19007245, >> 17385841). >> b) calibration method(s) by Zeiss and/or Leica that try to (but do >> not always) match output at all settings. >> See also >> >> >> high speed >> scanning has the potential to increase fluorescence yield and to reduce >> photobleaching. Borlinghaus RT. Microsc Res Tech. 2006 >> Sep;69(9):689-92.PMID: 16878313 >> >> >> >> >> >> >> >> >> At 12:37 PM 5/7/2010, you wrote: >> >> Dear list, >> >> I'm looking for general information about the comparison between the >> resonant scanners in the SP5 versus A1R. Apart other comments, are >> possible future upgrades by software in the image format, panning and >> quick switching between the high resolution scanner and the resonant >> one? >> >> >> Thank you very much in advance. >> >> >> -- >> >> >> Juan Luis Ribas >> >> Servicio de Microscopía >> >> Centro de Investigación, Tecnología e Innovación >> >> Universidad de Sevilla >> >> Av. Reina Mercedes 4b >> >> 41012 Sevilla >> >> >> |
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Jerry (Gerald) Sedgewick |
1st Annual Imaging in Research Workshop *commercial/academic announcement*
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Hello All,
44 percent of cases investigated by the Office of Research Integrity in 2005-6 involved accusations of image fraud, compared with about 6 percent a decade before that (1). These cases are rising, and most involve graduate and post-doctoral students. Students and staff who self-report familiarity with imaging programs like Photoshop may be using it improperly. In response to this trend, the "1st Annual Imaging in Research Course: Ethics, Acquisition, Post-Processing, Output and Segmenting" is being held at the University of Minnesota Continuing Education Center in Minneapolis/St. Paul, Minnesota, August 16 - 19, 2010, sponsored by the Histochemical Society and the Adobe Corporation. Attendees can choose to attend the course for 3- or 4-days. This workshop will educate those in science, medicine and engineering about correct techniques when acquiring, post-processing, and adjusting images for outputs; along with techniques that work for segmenting complex, biological images (for subsequent image analysis). Other benefits of taking the course will likely result in: Faster acceptance of submitted manuscripts Authors better able to demonstrate outcomes to their target audience Faster results from quantitation, with improved ability to segment desired features Better documentation of imaging procedures Standardization of post-processing Learning to adjust and modify images minimally and through the objective use of numbers. Jerry Sedgewick will present, along with invited speakers. Jerry directed a core light microscopy and imaging facility for 15 years at the University of Minnesota, published 2 books on Photoshop and digital imaging, and his quantitative work has led to FDA approval for start up companies. Please go to http://www.imagingandanalysis.com/seminars.html for more information. There is a limit of 30 seats. If you are a core facility director, you may also wish to pass this along to your users. Information and techniques learned in this course will aid in allaying concern about how your users acquire and post-process images; and will lead to better grant and publication opportunities, as well as improved images. The cost is $590 for 3-days and $840 for 4-days, U.S. dollars. It includes lunch, beverages and snacks. Registrations for those who received information about this course via their core facility will receive discounts. All the best, Jerry Sedgewick 1. "Journals Find Many Images in Research Are Faked," Jeffrey R. Young, The Chronicle of Higher Education, September 9, 2009) |
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RICHARD BURRY |
Re: 1st Annual Imaging in Research Workshop *commercial/academic announcement*
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Bill
I see the HCS is sponsoring a meeting on image ethics this summer. See bold below. Dick ----- Original Message ----- From: "Jerry (Gerald) Sedgewick" <[hidden email]> Date: Tuesday, May 25, 2010 12:34 pm Subject: 1st Annual Imaging in Research Workshop *commercial/academic announcement* To: [hidden email] > Hello All, > > 44 percent of cases investigated by the Office of Research > Integrity in > 2005-6 involved accusations of image fraud, compared with about > 6 percent > a decade before that (1). These cases are rising, and most involve > graduate and post-doctoral students. Students and staff > who self-report > familiarity with imaging programs like Photoshop may be using it > improperly. > > In response to this trend, the "1st Annual Imaging in Research Course: > Ethics, Acquisition, Post-Processing, Output and Segmenting" is being > held at the University of Minnesota Continuing Education Center in > Minneapolis/St. Paul, Minnesota, August 16 - 19, 2010, sponsored > by the > Histochemical Society and the Adobe Corporation. Attendees > can choose to > attend the course for 3- or 4-days. This workshop will > educate those in > science, medicine and engineering about correct techniques when > acquiring, post-processing, and adjusting images for outputs; > along with > techniques that work for segmenting complex, biological images (for > subsequent image analysis). > > Other benefits of taking the course will likely result in: > > > Faster acceptance of submitted manuscripts > > Authors better able to demonstrate outcomes to their target audience > > Faster results from quantitation, with improved ability to > segment > desired features > > Better documentation of imaging procedures > > Standardization of post-processing > > Learning to adjust and modify images minimally and through the > objective > use of numbers. > > Jerry Sedgewick will present, along with invited speakers. Jerry > directed a core light microscopy and imaging facility for 15 > years at the > University of Minnesota, published 2 books on Photoshop and digital > imaging, and his quantitative work has led to FDA approval for > start up > companies. > > Please go to http://www.imagingandanalysis.com/seminars.html for more > information. There is a limit of 30 seats. > > If you are a core facility director, you may also wish to pass > this along > to your users. Information and techniques learned in this > course will > aid in allaying concern about how your users acquire and post-process > images; and will lead to better grant and publication > opportunities, as > well as improved images. > > The cost is $590 for 3-days and $840 for 4-days, U.S. dollars. It > includes lunch, beverages and snacks. Registrations for > those who > received information about this course via their core facility will > receive discounts. > > All the best, > > Jerry Sedgewick > > 1. "Journals Find Many Images in Research Are Faked," > Jeffrey R. Young, The Chronicle of Higher Education, September > 9, 2009) > > > -- > BEGIN-ANTISPAM-VOTING-LINKS > ------------------------------------------------------ > > Teach CanIt if this mail (ID 1043290605) is spam: > Spam: > https://antispam.osu.edu/b.php?i=1043290605&m=855efe3218ec&c=sNot spam: https://antispam.osu.edu/b.php?i=1043290605&m=855efe3218ec&c=n > Forget vote: > https://antispam.osu.edu/b.php?i=1043290605&m=855efe3218ec&c=f--- > --------------------------------------------------- > END-ANTISPAM-VOTING-LINKS > Richard W. Burry, Ph.D. Department of Neuroscience, College of Medicine Campus Microscopy and Imaging Facility, Director The Ohio State University Associate Editor, Journal of Histochemistry and Cytochemistry 277 Biomedical Research Tower 460 West Twelfth Avenue Columbus, Ohio 43210 Voice 614.292.2814 Cell 614.638.3345 Fax 614.247.8849 |
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Roshma Azeem |
Re: Resonant scanner from A1R vs SP5 ...
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In reply to this post by Maclyn McCarty
Hi Mac, Fast acquisition time is required to analyze rapid biological processes in a cell. Resonant scanners are about 10 times faster compared to the speed of conventional scanners that are able to acquire fast frame recording and provide real time live images. Due to the faster frame rate, they are useful in resolving the complicated dynamic changes in living cells. Resonant scanners have some disadvantages like higher readout noise. In addition, their duty cycle is short as resonant scanners accelerate fast that may affect the scanning mirror . Further technical information can be seen at the following sites: http://www.microscopyu.com/tutorials/flash/resonantscanning/confocalresonantscanning/index.html http://www.microscopyu.com/articles/confocal/resonantscanning.html Nikon's A1R has a hybrid scanner and the SP5 has tandem scanner. Roshma. |
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George McNamara |
Re: Resonant scanner from A1R vs SP5 ...
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In reply to this post by Maclyn McCarty
Hi Mac,
On the Leica SP5 standard scanner can be operated from 100 Hz to 1400 Hz, with a default of 400 Hz (lines per second). The default (or less) is needed is you want to operate at 1x zoom - higher scan speeds use smaller field of views (you can zoom way up at any speed). Resonant scanner operates at 8000 Hz (lines per second). Upshot: resonant scanner operates at 5.7x faster than the fastest standard scanner speed. George George At 09:26 AM 5/24/2010, you wrote: What is the advantage of using resonant scanner? George McNamara, Ph.D. Image Core Manager Analytical Imaging Core Facility University of Miami, Miller School of Medicine Miami, FL 33136 [hidden email] [hidden email] 305-243-8436 office http://www.sylvester.org/AICF (Analytical Imaging Core Facility) http://www.sylvester.org/AICF/pubspectra.zip (the entire 2000+ spectra .xlsx file is in the zip file) http://home.earthlink.net/~geomcnamara |
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David Strachan |
Re: Resonant scanner from A1R vs SP5 ...
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I am sure you can just type in the box to select other values than the
nice round numbers given originally, even down to 1 hertz. See below….. On the Leica SP5 standard scanner
can be operated from 100 Hz to 1400 Hz, David Strachan The Beatson Institute
for Cancer Research ( Direct Line +44 (0) 141 330 6872 ( Fax +44 (0) 141 942 6521 * E-mail [hidden email] From:
Confocal Microscopy List [mailto:[hidden email]] On Behalf Of George McNamara Hi Mac, What is the advantage of
using resonant scanner? From: George McNamara
<[hidden email]> Subject: Re: Resonant
scanner from A1R vs SP5 ... To:
[hidden email] Date: Saturday, 8 May, 2010, 2:45 PM Hi Juan, On the SP5, when you start LAS AF the choice is
resonant scanner or high resolution scanner. No way currently to quickly
switch, but do you really need to? If you have a motorized stage, you can tile
scan in resonant scanner mode faster than you could exit and restart. George p.s. I've noticed on both
the Leica SP5, Zeiss LSM510 and Zeiss LSM710 that "faster is brighter"
for many fluorophores. That is, a 0.4 us dwell time results in a brighter image
than a 4 us which is brighter than a 40 us. This implies that <0.4 us might
be even brighter - i.e. resonant scanner mode. A couple of possible
explanations (not mutually exclusive): a) photophysics - re TRex
(PubMed 19337661), papers by Sanden/Spielmann/Widergren et al (PubMed 20375039,
20196585, 19007245, 17385841). b) calibration method(s)
by Zeiss and/or Leica that try to (but do not always) match output at all
settings. See also high speed scanning
has the potential to increase fluorescence yield and to reduce photobleaching.
Borlinghaus RT. Microsc Res Tech. 2006 Sep;69(9):689-92.PMID: 16878313 At 12:37 PM 5/7/2010, you
wrote: Dear list, I'm looking for general information about the
comparison between the resonant scanners in the SP5 versus A1R. Apart other
comments, are possible future upgrades by software in the image format, panning
and quick switching between the high resolution scanner and the resonant one? Thank you very much in advance. -- Juan Luis Ribas Servicio de Microscopía Centro de Investigación,
Tecnología e Innovación Universidad de Sevilla Av. Reina Mercedes 4b 41012 Sevilla
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