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Dear Colleagues,
Just wanted to share a few thoughts with anyone who is interested. In 1991 Paul Adams [SUNY-SB] had an MRC600 sitting quietly in a lab humming with electrophysiologists. By good fortune, Barry Burbach, SuperTech, took me, a truly green PhD, under his wing and I had the the most enjoyable years studying nuclear calcium transients, neuronal population imaging, thalamic brain slices and more. This was all built on the shoulders of Roger Tsien: BAPTA, fura-2, fluo-3, etc., etc. and because of his tools (in no small measure), I was able to land, in 1997, at Northeastern University as an Asst. Professor. His tools continued to drive my research (e.g. EGFP) and I started teaching a course called Biological Imaging that was largely inspired by Roger. FRET imaging of cAMP, GFP mutations and the calcium-imaging protein Cameleon (my favorite Tsien papers to present) revealed to the world what was possible when fluorophore and molecular biology met confocal microscope. I could easily have taught an entire course based just upon Roger's work. For many years I asked (my students) when will Roger Tsien win HIS Nobel Prize? That was a joyous day for me. This is a sad one. But the light that Roger shown on cells and brain slices and zebrafish will never be extinguished.
Donald M. O'Malley
Assoc. Professor
Dept. Biology & Program in Behavioral Neuroscience
Northeastern University
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617-373-2284
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