Rotate microscope to view vertical specimen
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Richard Superfine |
Rotate microscope to view vertical specimen
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We are interested in viewing a cell culture that has been turned vertically
so that the surface liquid can drain. We therefore need to rotate the microscope so that its optical axis is horizontal. We want to be able to rotate the microscope continuously from a vertical optical axis (normal configuration) to a horizontal one. Does anyone know of a publication that describes the mechanical design of a rotation assembly for accomplishing this? Thanks, Rich Richard Superfine Bowman and Gordon Gray Professor Department of Physics and Astronomy Director, Center for Computer Integrated Systems for Microscopy and Manipulation (CISMM) Phillips Hall CB3255 University of North Carolina Chapel Hill, NC 27599-3255 919.962.1185 CISMM.org |
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Rosemary.White |
Re: Rotate microscope to view vertical specimen
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Dear Richard,
Nina Allen, at UNC Raleigh, has a setup like this, I believe, used to study plant responses to gravity. If I remember correctly, one of her confocals is on its side, too. cheers, Rosemary Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia ph 61 2 6246 5475 fx 61 2 6246 5334 On 15/04/09 2:26 PM, "Richard Superfine" <[hidden email]> wrote: > We are interested in viewing a cell culture that has been turned vertically > so that the surface liquid can drain. We therefore need to rotate the > microscope so that its optical axis is horizontal. We want to be able to > rotate the microscope continuously from a vertical optical axis (normal > configuration) to a horizontal one. Does anyone know of a publication that > describes the mechanical design of a rotation assembly for accomplishing this? > Thanks, > Rich > > Richard Superfine > Bowman and Gordon Gray Professor > Department of Physics and Astronomy > Director, Center for Computer Integrated Systems for Microscopy and > Manipulation (CISMM) Phillips Hall CB3255 University of North Carolina > Chapel Hill, NC 27599-3255 > 919.962.1185 CISMM.org |
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Rosemary.White |
Re: Rotate microscope to view vertical specimen
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Oops, it's NCSU, not UNC at Raleigh.... The tyranny of (quite long)
distance... On 15/04/09 1:54 PM, "Rosemary White" <[hidden email]> wrote: > Dear Richard, > > Nina Allen, at UNC Raleigh, has a setup like this, I believe, used to study > plant responses to gravity. If I remember correctly, one of her confocals > is on its side, too. > > cheers, > Rosemary > > Rosemary White > CSIRO Plant Industry > GPO Box 1600 > Canberra, ACT 2601 > Australia > > ph 61 2 6246 5475 > fx 61 2 6246 5334 > > > > On 15/04/09 2:26 PM, "Richard Superfine" <[hidden email]> wrote: > >> We are interested in viewing a cell culture that has been turned vertically >> so that the surface liquid can drain. We therefore need to rotate the >> microscope so that its optical axis is horizontal. We want to be able to >> rotate the microscope continuously from a vertical optical axis (normal >> configuration) to a horizontal one. Does anyone know of a publication that >> describes the mechanical design of a rotation assembly for accomplishing >> this? >> Thanks, >> Rich >> >> Richard Superfine >> Bowman and Gordon Gray Professor >> Department of Physics and Astronomy >> Director, Center for Computer Integrated Systems for Microscopy and >> Manipulation (CISMM) Phillips Hall CB3255 University of North Carolina >> Chapel Hill, NC 27599-3255 >> 919.962.1185 CISMM.org |
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Stephen Cody-2 |
Re: Rotate microscope to view vertical specimen
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In reply to this post by Richard Superfine
Dear Richard,
A simple and fairly cost effective way to do it may be with the "Objective Inverter"
The name implies inverting the objective. But I think it is capable of turning the objective to any angle.
No commercial interest, and I have not used this system either, but it looks very cleaver.
Cheers
Steve Cody
2009/4/15 Richard Superfine <[hidden email]> We are interested in viewing a cell culture that has been turned vertically -- Stephen H. Cody Consulting Microscopist |
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Andrew Resnick |
Re: Rotate microscope to view vertical specimen
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In reply to this post by Richard Superfine
A colleague at NASA was studying colloidal crystals, and because of
sedimentation had the exact same situation. He was using a Zeiss Axiskop, which had a well-shaped base that could conveniently be anchored/clamped to a plate. The entire assembly could be rotated by hand, and the final orientation held in place by use of a automobile drum brake. I'll see if he's still around and put him in touch. Andy At 11:26 PM 4/14/2009, you wrote: >We are interested in viewing a cell culture that has been turned vertically >so that the surface liquid can drain. We therefore need to rotate the >microscope so that its optical axis is horizontal. We want to be able to >rotate the microscope continuously from a vertical optical axis (normal >configuration) to a horizontal one. Does anyone know of a publication that >describes the mechanical design of a rotation assembly for accomplishing this? >Thanks, >Rich > >Richard Superfine >Bowman and Gordon Gray Professor >Department of Physics and Astronomy >Director, Center for Computer Integrated Systems for Microscopy and >Manipulation (CISMM) Phillips Hall CB3255 University of North Carolina >Chapel Hill, NC 27599-3255 >919.962.1185 CISMM.org Andrew Resnick, Ph. D. Instructor Department of Physiology and Biophysics Case Western Reserve University 216-368-6899 (V) 216-368-4223 (F) |
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Nina Allen |
Re: Rotate microscope to view vertical specimen
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In reply to this post by Rosemary.White
This is correct and it still works.
Nina On 4/14/09 10:54 PM, "Rosemary White" <[hidden email]> wrote: > Dear Richard, > > Nina Allen, at UNC Raleigh, has a setup like this, I believe, used to study > plant responses to gravity. If I remember correctly, one of her confocals > is on its side, too. > > cheers, > Rosemary > > Rosemary White > CSIRO Plant Industry > GPO Box 1600 > Canberra, ACT 2601 > Australia > > ph 61 2 6246 5475 > fx 61 2 6246 5334 > > > > On 15/04/09 2:26 PM, "Richard Superfine" <[hidden email]> wrote: > >> We are interested in viewing a cell culture that has been turned vertically >> so that the surface liquid can drain. We therefore need to rotate the >> microscope so that its optical axis is horizontal. We want to be able to >> rotate the microscope continuously from a vertical optical axis (normal >> configuration) to a horizontal one. Does anyone know of a publication that >> describes the mechanical design of a rotation assembly for accomplishing >> this? >> Thanks, >> Rich >> >> Richard Superfine >> Bowman and Gordon Gray Professor >> Department of Physics and Astronomy >> Director, Center for Computer Integrated Systems for Microscopy and >> Manipulation (CISMM) Phillips Hall CB3255 University of North Carolina >> Chapel Hill, NC 27599-3255 >> 919.962.1185 CISMM.org > Nina Stromgren Allen Professor Emerita Department of Plant Biology North Carolina State University Raleigh, NC 27695-7612 Phone: 919-515-8382,2727 Fax: 919-515-3436 E-mail: [hidden email] ***************************************************** |
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Nina Allen |
Re: Rotate microscope to view vertical specimen
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In reply to this post by Rosemary.White
Duly noted and NC State did not win the basketball championship ...that
other school did.... Nina On 4/14/09 11:05 PM, "Rosemary White" <[hidden email]> wrote: > Oops, it's NCSU, not UNC at Raleigh.... The tyranny of (quite long) > distance... > > > On 15/04/09 1:54 PM, "Rosemary White" <[hidden email]> wrote: > >> Dear Richard, >> >> Nina Allen, at UNC Raleigh, has a setup like this, I believe, used to study >> plant responses to gravity. If I remember correctly, one of her confocals >> is on its side, too. >> >> cheers, >> Rosemary >> >> Rosemary White >> CSIRO Plant Industry >> GPO Box 1600 >> Canberra, ACT 2601 >> Australia >> >> ph 61 2 6246 5475 >> fx 61 2 6246 5334 >> >> >> >> On 15/04/09 2:26 PM, "Richard Superfine" <[hidden email]> wrote: >> >>> We are interested in viewing a cell culture that has been turned vertically >>> so that the surface liquid can drain. We therefore need to rotate the >>> microscope so that its optical axis is horizontal. We want to be able to >>> rotate the microscope continuously from a vertical optical axis (normal >>> configuration) to a horizontal one. Does anyone know of a publication that >>> describes the mechanical design of a rotation assembly for accomplishing >>> this? >>> Thanks, >>> Rich >>> >>> Richard Superfine >>> Bowman and Gordon Gray Professor >>> Department of Physics and Astronomy >>> Director, Center for Computer Integrated Systems for Microscopy and >>> Manipulation (CISMM) Phillips Hall CB3255 University of North Carolina >>> Chapel Hill, NC 27599-3255 >>> 919.962.1185 CISMM.org > Nina Stromgren Allen Professor Emerita Department of Plant Biology North Carolina State University Raleigh, NC 27695-7612 Phone: 919-515-8382,2727 Fax: 919-515-3436 E-mail: [hidden email] ***************************************************** |
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Knecht, David |
Re: Rotate microscope to view vertical specimen
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Your speaking of the University of Connecticut, of course! Dave
On Apr 15, 2009, at 2:46 PM, Nina Allen wrote:
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax) |
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Robert Peterson-3-3 |
Re: Rotate microscope to view vertical specimen
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Congrats to the Lady .... Connecticutters?
JK. A great year for the Huskies, nonetheless - Go Heels!!! No hard feelings towards those "other" local sports teams, my wife is a wolfpack grad and I spent 5 years at UNC-NJ, I mean "Duke". Sent from my Verizon Wireless BlackBerry From: David Knecht Date: Wed, 15 Apr 2009 17:31:04 -0400 To: <[hidden email]> Subject: Re: Rotate microscope to view vertical specimen Your speaking of the University of Connecticut, of course! Dave On Apr 15, 2009, at 2:46 PM, Nina Allen wrote:
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax) |
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Edelmann, Richard E. Dr. |
Re: Rotate microscope to view vertical specimen
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In reply to this post by Stephen Cody-2
We "rotated" a confocal a number of years ago. This "Objective
Inverter" seems like an easier way. The biggest issue is that the focus rack and gears are designed for a verticle motion. There is A lot of whiplash when changing focus directions. Our system has approximately 185 µm of slack before changing directions in focusing. So you are pretty much lmited to focusing in one direction. For z-series you always have to reset the focus gearing since the normal stepers etc do not take into accoutn a the large whiplash. Oh, ours is an older PCM2000 on a Nikon Optiphot. On 15 Apr 2009 at 1:12, Stephen Cody wrote: > Dear Richard, > > A simple and fairly cost effective way to do it may be with the "Objective Inverter" > http://www.lsmtech.com/objectiveinverter.html > > The name implies inverting the objective. But I think it is capable of turning the objective to any angle. > > No commercial interest, and I have not used this system either, but it looks very cleaver. > > Cheers > Steve Cody > > 2009/4/15 Richard Superfine <[hidden email]<mailto:[hidden email]>> > We are interested in viewing a cell culture that has been turned vertically > so that the surface liquid can drain. We therefore need to rotate the > microscope so that its optical axis is horizontal. We want to be able to > rotate the microscope continuously from a vertical optical axis (normal > configuration) to a horizontal one. Does anyone know of a publication that > describes the mechanical design of a rotation assembly for accomplishing this? > Thanks, > Rich > > Richard Superfine > Bowman and Gordon Gray Professor > Department of Physics and Astronomy > Director, Center for Computer Integrated Systems for Microscopy and > Manipulation (CISMM) Phillips Hall CB3255 University of North Carolina > Chapel Hill, NC 27599-3255 > 919.962.1185 CISMM.org > > > > -- > Stephen H. Cody > Consulting Microscopist > Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: [hidden email] http://www.emf.muohio.edu |
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Craig Brideau |
Re: Rotate microscope to view vertical specimen
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When you are operating horizontally you have to use springs to keep
tension on the gears at all times. When you are vertical gravity does this for you. Craig On Thu, Apr 16, 2009 at 11:31 AM, Richard E. Edelmann <[hidden email]> wrote: > We "rotated" a confocal a number of years ago. This "Objective > Inverter" seems like an easier way. > > The biggest issue is that the focus rack and gears are designed for a > verticle motion. There is A lot of whiplash when changing focus > directions. Our system has approximately 185 µm of slack before > changing directions in focusing. So you are pretty much lmited to > focusing in one direction. For z-series you always have to reset the > focus gearing since the normal stepers etc do not take into accoutn a > the large whiplash. > > Oh, ours is an older PCM2000 on a Nikon Optiphot. > > > > > > On 15 Apr 2009 at 1:12, Stephen Cody wrote: > >> Dear Richard, >> >> A simple and fairly cost effective way to do it may be with the "Objective Inverter" >> http://www.lsmtech.com/objectiveinverter.html >> >> The name implies inverting the objective. But I think it is capable of turning the objective to any angle. >> >> No commercial interest, and I have not used this system either, but it looks very cleaver. >> >> Cheers >> Steve Cody >> >> 2009/4/15 Richard Superfine <[hidden email]<mailto:[hidden email]>> >> We are interested in viewing a cell culture that has been turned vertically >> so that the surface liquid can drain. We therefore need to rotate the >> microscope so that its optical axis is horizontal. We want to be able to >> rotate the microscope continuously from a vertical optical axis (normal >> configuration) to a horizontal one. Does anyone know of a publication that >> describes the mechanical design of a rotation assembly for accomplishing this? >> Thanks, >> Rich >> >> Richard Superfine >> Bowman and Gordon Gray Professor >> Department of Physics and Astronomy >> Director, Center for Computer Integrated Systems for Microscopy and >> Manipulation (CISMM) Phillips Hall CB3255 University of North Carolina >> Chapel Hill, NC 27599-3255 >> 919.962.1185 CISMM.org >> >> >> >> -- >> Stephen H. Cody >> Consulting Microscopist >> > > > Richard E. Edelmann, Ph.D. > EXPO Editor, Microscopy and Microanalysis Supplement > Electron Microscopy Facility Director > 364 Pearson Hall > Miami University, Oxford, OH 45056 > Ph: 513.529.5712 Fax: 513.529.4243 > E-mail: [hidden email] > http://www.emf.muohio.edu > |
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