SHG

[Sarah Kefayati]

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Hello all,

 

I appreciate if you could help me with my question:

 

I just want to make sure about this fact that by using a non-linear crystal in the path of my Ti-sapphire laser I will have the frequency doubled beam which excites my sample via traditional one-photon excitation.

But dose the intensity of the emitted fluorescence depend on excitation power linearly or quadratically?

 

any information in this regard is highly appreciated.

 

Thanks

Sarah

[Peng Xi-2]

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Hi Sarah,
    Just like you asked, it will be an one-photon excitation. So,
although it is possible that you create a femtosecond 400nm laser pulse,
it is still linear excitation -- one photon process. Thank you!
    I am very interested in the experiment itself -- have you tried to
put a SHG crystal inside the Ti:Sa laser cavity and see the 400nm emission?

Best regards,
Peng Xi
Associate Professor
Institute for Laser Medicine and Biophotonics
Shanghai Jiao Tong University
800 Dongchuan Rd.
Shanghai 200240, China
Tel: [phone]
http://biophotonics.sjtu.edu.cn/



Sarah Kefayati wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Hello all,
>  
> I appreciate if you could help me with my question:
>  
> I just want to make sure about this fact that by using a non-linear
> crystal in the path of my Ti-sapphire laser I will have the frequency
> doubled beam which excites my sample via traditional one-photon
> excitation.
> But dose the intensity of the emitted fluorescence depend on
> excitation power linearly or quadratically?
>  
> any information in this regard is highly appreciated.
>  
> Thanks
> Sarah