*****
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*****
We've had great success with DABCO antifade in TDE mounting media. See
protocol here:
http://hcbi.fas.harvard.edu/files/hcbi_recommended_mounting_media_0.pdfThe refractive index match (mounting medium/coverslip/oil) greatly improves
the achievable resolution and reduces processing artifacts.
-Doug
On Thu, Sep 3, 2015 at 8:19 AM, echatre <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Hello,
>
> We are looking for an antifading which would be effective with a far-red
> dye, for instance AF647 or Cy5, for SIM experiments (with Elyra system from
> Zeiss).
> We have already tested FMG and PBS + glycerol but they were ineffective.
>
> Thanks for your help.
> elodie chatre
>
> --
> Elodie Chatre
>
> PLATIM / Lyon Bio Image facility
> US8 / UMS3444 BioSciences
> Ecole Normale Supérieure de Lyon
> 46 allée d'Italie, 69007 Lyon
>
> tel : +33 4.72.72.87.62
> fax : +33 4.72.72.87.66
>
http://www.sfr-biosciences.fr/plateformes/Phenotypage-cellulaire/PLATIM>
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