SNR

[Sarah Kefayati]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello all,

 

I need to calculate signal to background as I am going through the depth.I am using a Tetraspeck beads injected to the arabdiopsis leaf to test this.So far I have done that with this way:I have cropped the beads in the different depth and I have use the simple standard deviation to show that I am loosing my signal as I am going through the depth.But I don't think that is a good scale of SNR.There is an article http://www.biophysj.org/cgi/rapidpdf/biophysj.106.102459v1 

They have calculated exactly what I am looking for with the same approch of injecting beads to the OHBS and calculating the SNR in each depth.But the method that they have calculated SNR is not clear for me.I appreciate if you could check the paper and give me any idea that you get.I tried to contact the author,no luck!

 

Thanks

Sarah

[Zoltan]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Sarah,

If I may be so bold as to point out that the lack of response to your post may reflect that you perhaps overwhelmed the forum with your endless supply of questions.  We all like to rely on help from the numerous and very generous experts on this wonderful forum, but we also do try to solve our problems first, and only address the forum if we ran out of ideas.  Just my 2c, of course.    

    Best,

Zoltan

On Fri, May 16, 2008 at 10:42 PM, Sarah Kefayati <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello all,

 

I need to calculate signal to background as I am going through the depth.I am using a Tetraspeck beads injected to the arabdiopsis leaf to test this.So far I have done that with this way:I have cropped the beads in the different depth and I have use the simple standard deviation to show that I am loosing my signal as I am going through the depth.But I don't think that is a good scale of SNR.There is an article http://www.biophysj.org/cgi/rapidpdf/biophysj.106.102459v1 

They have calculated exactly what I am looking for with the same approch of injecting beads to the OHBS and calculating the SNR in each depth.But the method that they have calculated SNR is not clear for me.I appreciate if you could check the paper and give me any idea that you get.I tried to contact the author,no luck!

 

Thanks

Sarah

--
--
Zoltan Cseresnyes
Facility manager, Imaging Suite

[Sarah Kefayati]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Zoltan,

 

Thank you for your comment and your concern.

 

But I just want to point out that it would be nice if we try not to prejudge a person by the emails and the number of the questions. First:I am sending emails asking not only my questions but also the questions from different people in our lab.

second,you do not know me ,how did you come to the conclusion that I am not trying to find my answer first myself?

and third,question is question,it dose not matter if two persons asking 2 questions or one person asking 2 question.I have not been told that there is a limit in the number of the questions.there is no obligation in the forum that people should answer every question that has been ask if someone knows something and feels like to share he will.

 

I appreciate if you do not discourage a student with this prejudged comments in the academic forum.

 

Regards

Sarah

 

On Fri, May 16, 2008 at 7:45 PM, Zoltan Cseresnyes <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Sarah,

If I may be so bold as to point out that the lack of response to your post may reflect that you perhaps overwhelmed the forum with your endless supply of questions.  We all like to rely on help from the numerous and very generous experts on this wonderful forum, but we also do try to solve our problems first, and only address the forum if we ran out of ideas.  Just my 2c, of course.    

    Best,

Zoltan

On Fri, May 16, 2008 at 10:42 PM, Sarah Kefayati <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello all,

 

I need to calculate signal to background as I am going through the depth.I am using a Tetraspeck beads injected to the arabdiopsis leaf to test this.So far I have done that with this way:I have cropped the beads in the different depth and I have use the simple standard deviation to show that I am loosing my signal as I am going through the depth.But I don't think that is a good scale of SNR.There is an article http://www.biophysj.org/cgi/rapidpdf/biophysj.106.102459v1 

They have calculated exactly what I am looking for with the same approch of injecting beads to the OHBS and calculating the SNR in each depth.But the method that they have calculated SNR is not clear for me.I appreciate if you could check the paper and give me any idea that you get.I tried to contact the author,no luck!

 

Thanks

Sarah

--
--
Zoltan Cseresnyes
Facility manager, Imaging Suite

[Guy Cox]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Sarah,

 

          One reason there may not have been much response on this

is that there was a detailed discussion of calculation of SNR on this

list fairly recently.  It ended up so mathematical that mere biologists

like me would not dare to comment on the topic again!  If you look

through the archives I'm sure you'll find it quite easily, and there may

be something there that will help you.

 

                                                                                   Guy

 

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone [phone]     Fax [phone]
Mobile [phone]
______________________________________________
     http://www.guycox.net

 

---

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sarah Kefayati
Sent: Saturday, 17 May 2008 10:02 AM
To: [hidden email]
Subject: Re: SNR

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Zoltan,

 

Thank you for your comment and your concern.

 

But I just want to point out that it would be nice if we try not to prejudge a person by the emails and the number of the questions. First:I am sending emails asking not only my questions but also the questions from different people in our lab.

second,you do not know me ,how did you come to the conclusion that I am not trying to find my answer first myself?

and third,question is question,it dose not matter if two persons asking 2 questions or one person asking 2 question.I have not been told that there is a limit in the number of the questions.there is no obligation in the forum that people should answer every question that has been ask if someone knows something and feels like to share he will.

 

I appreciate if you do not discourage a student with this prejudged comments in the academic forum.

 

Regards

Sarah

 

On Fri, May 16, 2008 at 7:45 PM, Zoltan Cseresnyes <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Sarah,

If I may be so bold as to point out that the lack of response to your post may reflect that you perhaps overwhelmed the forum with your endless supply of questions.  We all like to rely on help from the numerous and very generous experts on this wonderful forum, but we also do try to solve our problems first, and only address the forum if we ran out of ideas.  Just my 2c, of course.    

    Best,

Zoltan

On Fri, May 16, 2008 at 10:42 PM, Sarah Kefayati <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello all,

 

I need to calculate signal to background as I am going through the depth.I am using a Tetraspeck beads injected to the arabdiopsis leaf to test this.So far I have done that with this way:I have cropped the beads in the different depth and I have use the simple standard deviation to show that I am loosing my signal as I am going through the depth.But I don't think that is a good scale of SNR.There is an article http://www.biophysj.org/cgi/rapidpdf/biophysj.106.102459v1 

They have calculated exactly what I am looking for with the same approch of injecting beads to the OHBS and calculating the SNR in each depth.But the method that they have calculated SNR is not clear for me.I appreciate if you could check the paper and give me any idea that you get.I tried to contact the author,no luck!

 

Thanks

Sarah

--
--
Zoltan Cseresnyes
Facility manager, Imaging Suite

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[J.P. Shields]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Wellll… I was going to ask a question, but, ah, maybe I’ll just go to the M&M listserver instead.

John

 

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Zoltan Cseresnyes
Sent: Friday, May 16, 2008 7:45 PM
To: [hidden email]
Subject: Re: SNR

 

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Sarah,

 

If I may be so bold as to point out that the lack of response to your post may reflect that you perhaps overwhelmed the forum with your endless supply of questions.  We all like to rely on help from the numerous and very generous experts on this wonderful forum, but we also do try to solve our problems first, and only address the forum if we ran out of ideas.  Just my 2c, of course.    

    Best,

 

Zoltan

 

 

On Fri, May 16, 2008 at 10:42 PM, Sarah Kefayati <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello all,

 

I need to calculate signal to background as I am going through the depth.I am using a Tetraspeck beads injected to the arabdiopsis leaf to test this.So far I have done that with this way:I have cropped the beads in the different depth and I have use the simple standard deviation to show that I am loosing my signal as I am going through the depth.But I don't think that is a good scale of SNR.There is an article http://www.biophysj.org/cgi/rapidpdf/biophysj.106.102459v1 

They have calculated exactly what I am looking for with the same approch of injecting beads to the OHBS and calculating the SNR in each depth.But the method that they have calculated SNR is not clear for me.I appreciate if you could check the paper and give me any idea that you get.I tried to contact the author,no luck!

 

Thanks

Sarah

--
--
Zoltan Cseresnyes
Facility manager, Imaging Suite

--
This message has been scanned for viruses and
dangerous content by MailScanner, and is
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[José M. Pellegrino]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Are you kidding?? This is a serious forum!!

José

----- Original Message -----

From: [hidden email]

To: [hidden email]

Sent: Wednesday, May 21, 2008 11:20 AM

Subject: Re: SNR

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Wellll… I was going to ask a question, but, ah, maybe I’ll just go to the M&M listserver instead.

John

 

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Zoltan Cseresnyes
Sent: Friday, May 16, 2008 7:45 PM
To: [hidden email]
Subject: Re: SNR

 

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Sarah,

 

If I may be so bold as to point out that the lack of response to your post may reflect that you perhaps overwhelmed the forum with your endless supply of questions.  We all like to rely on help from the numerous and very generous experts on this wonderful forum, but we also do try to solve our problems first, and only address the forum if we ran out of ideas.  Just my 2c, of course.    

    Best,

 

Zoltan

 

 

On Fri, May 16, 2008 at 10:42 PM, Sarah Kefayati <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello all,

 

I need to calculate signal to background as I am going through the depth.I am using a Tetraspeck beads injected to the arabdiopsis leaf to test this.So far I have done that with this way:I have cropped the beads in the different depth and I have use the simple standard deviation to show that I am loosing my signal as I am going through the depth.But I don't think that is a good scale of SNR.There is an article http://www.biophysj.org/cgi/rapidpdf/biophysj.106.102459v1 

They have calculated exactly what I am looking for with the same approch of injecting beads to the OHBS and calculating the SNR in each depth.But the method that they have calculated SNR is not clear for me.I appreciate if you could check the paper and give me any idea that you get.I tried to contact the author,no luck!

 

Thanks

Sarah

--
--
Zoltan Cseresnyes
Facility manager, Imaging Suite

__________ Información de NOD32, revisión 3116 (20080521) __________

Este mensaje ha sido analizado con NOD32 antivirus system
http://www.nod32.com

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This message has been scanned for viruses and
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[Dale Callaham]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Ok, Zoltan made a fairly insensitive, inconsiderate rebuff and I don't
think it is representative of the Confocal Listserver sensibilities on
the whole. It is always appropriate to do your homework first, but this
forum should also be congenial and there are probably better ways to
have made his point. Now this is degenerating into a chat, dissin' the
Microcopy List, and filling my mailbox with unnecessary bytes. Can we
please move on?

Thanks,

Dale

José M. Pellegrino wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Are you kidding?? This is a serious forum!!
> José
>
>     ----- Original Message -----
>     *From:* J.P. Shields <mailto:[hidden email]>
>     *To:* [hidden email]
>     <mailto:[hidden email]>
>     *Sent:* Wednesday, May 21, 2008 11:20 AM
>     *Subject:* Re: SNR
>
>     Search the CONFOCAL archive at
>     http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>     Wellll… I was going to ask a question, but, ah, maybe I’ll just go
>     to the M&M listserver instead.
>
>     John
>
>      
>
>     *From:* Confocal Microscopy List
>     [mailto:[hidden email]] *On Behalf Of *Zoltan Cseresnyes
>     *Sent:* Friday, May 16, 2008 7:45 PM
>     *To:* [hidden email]
>     <mailto:[hidden email]>
>     *Subject:* Re: SNR
>
>      
>
>     Search the CONFOCAL archive at
>     http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>     Sarah,
>
>      
>
>     If I may be so bold as to point out that the lack of response to
>     your post may reflect that you perhaps overwhelmed the forum with
>     your endless supply of questions.  We all like to rely on help from
>     the numerous and very generous experts on this wonderful forum, but
>     we also do try to solve our problems first, and only address the
>     forum if we ran out of ideas.  Just my 2c, of course.    
>
>         Best,
>
>      
>
>     Zoltan
>
>      
>
>      
>
>     On Fri, May 16, 2008 at 10:42 PM, Sarah Kefayati <[hidden email]
>     <mailto:[hidden email]>> wrote:
>
>     Search the CONFOCAL archive at
>     http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>         Hello all,
>
>          
>
>         I need to calculate signal to background as I am going through
>         the depth.I am using a Tetraspeck beads injected to the
>         arabdiopsis leaf to test this.So far I have done that with this
>         way:I have cropped the beads in the different depth and I have
>         use the simple standard deviation to show that I am loosing my
>         signal as I am going through the depth.But I don't think that is
>         a good scale of SNR.There is an
>         article http://www.biophysj.org/cgi/rapidpdf/biophysj.106.102459v1 
>
>         They have calculated exactly what I am looking for with the same
>         approch of injecting beads to the OHBS and calculating the SNR
>         in each depth.But the method that they have calculated SNR is
>         not clear for me.I appreciate if you could check the paper and
>         give me any idea that you get.I tried to contact the author,no luck!
>
>          
>
>         Thanks
>
>         Sarah
>
>
>
>
>     --
>     --
>     Zoltan Cseresnyes
>     Facility manager, Imaging Suite
>
>
>
>     __________ Información de NOD32, revisión 3116 (20080521) __________
>
>     Este mensaje ha sido analizado con NOD32 antivirus system
>     http://www.nod32.com
>
>     --
>     This message has been scanned for viruses and
>     dangerous content by *MailScanner* <http://www.mailscanner.info/>,
>     and is
>     believed to be clean.