SSD-RAID for new wide-field microscopy system

[RJ3]

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SSDs on an acquisition computer really only need to be large enough in capacity for about a day's worth of experiments (obviously more is convenient).

The SSDs write speed also needs to be fast enough to not be a bottleneck in your work flow. As someone else pointed out, a 10-link camlink framegrabber like the BitFlow Carbon that is used with the Andor Zyla will be bottlenecked by a SATA III SSD.

A single NVMe 4-lane PCIe SSD will pretty much beat 4x SATA III drives in RAID 0. SATA III has made the way of the dinosaur, and should not be used in new systems unless you have capacity or budgeting concerns. SATA III supports 500 MB/sec write, 4 in a RAID 0 will support about 2000 MB/sec.

If you need more throughput than about 2000 MB/sec writes, which a single NVMe x4 can handle by itself, you should look into RAID 0 of NVMe drives. There are PCIe cards that fit in a 16-lane slot which can accommodate up to 4 NVMe drives (with 4-lanes each) in RAID 0 configuration.

Of course you can look at things like sequential vs. random writes, real-world benchmarks etc, so these are estimations.

[Ralf Palmisano]

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Hi Mirco,

I am literally signing everything Nuno Mureno wrote. In addition whoever is the manufatcurer. I consider it bad advise to invest in an 11 GB graphics card and even worse to call 6.000$ for a 2 TB SSD extension. That is just playing foul or they have no clue about what they are selling and why...

My pennie in here

Ralph

Am 23/05/2019 um 14:57 schrieb Nuno Moreno:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Mirco
>
> Honestly I see no reason to have so much memory and such a high spec graphics card for an acquisition computer. Regarding the HDs, I would keep the 2TB and add extra space non SSD. Then just automate data flow from the fast to the slow drive. 2TB extra for 6000$ is just too much and might be too short anyway.
>
> Just my 2 cents
> \N
>
>
>
>> On 23 May 2019, at 14:38, Mirco Martino <[hidden email]> wrote:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi all,
>>
>> we are buying a new fluorescent microscope wide-field system. The system include a workstation with 64GB DDR4 RAM, 11GB of graphic card, 512 gb of memory and SSD-RAID of 2TB. There is an option to increase the SSD-RAID to 4TB for  about 6000$ extra. The system will be use for both fixed materiel imaging and for live-imaging with time-laps, z-stack, tiling and multi-fluorophores experiments. I was wondering if it is worth to invest those extra money for this option or not. This will be the first time we will have time-laps experiments, so I don't know what to expect as pictures size.
>>
>> What do you think?
>>
>> Thanks in advance for your comments and opinions!
>>

--
Ralf Palmisano
Head - Optical Imaging Centre Erlangen

Fellow Royal Microscopical Society
Member Royal Society of Medicine

Speaker Scientific Advisory Board "German Society for Microscopy and Image Analysis"
Board of Directors "Core Technologies for Life Sciences"

Cauerstr. 3
91058 Erlangen

[phone] (Secretary)
[phone] (Office)

[Julia Edgar]

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Dear All
A very naive question I know...

How do you display time-lapse combined with confocal z stack? Flatten the z stack and play the time-lapse movie of the flattened (maximum intensity projection?) images?

If so, how do you generate the movie using Zen software and/or Fiji?

Many thanks in advance.
Julia

Get Outlook for iOS<https://aka.ms/o0ukef>

________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Ralf Palmisano <[hidden email]>
Sent: Saturday, May 25, 2019 1:47 am
To: [hidden email]
Subject: Re: SSD-RAID for new wide-field microscopy system

*****
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*****

Hi Mirco,

I am literally signing everything Nuno Mureno wrote. In addition whoever is the manufatcurer. I consider it bad advise to invest in an 11 GB graphics card and even worse to call 6.000$ for a 2 TB SSD extension. That is just playing foul or they have no clue about what they are selling and why...

My pennie in here

Ralph

Am 23/05/2019 um 14:57 schrieb Nuno Moreno:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Mirco
>
> Honestly I see no reason to have so much memory and such a high spec graphics card for an acquisition computer. Regarding the HDs, I would keep the 2TB and add extra space non SSD. Then just automate data flow from the fast to the slow drive. 2TB extra for 6000$ is just too much and might be too short anyway.
>
> Just my 2 cents
> \N
>
>
>
>> On 23 May 2019, at 14:38, Mirco Martino <[hidden email]> wrote:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi all,
>>
>> we are buying a new fluorescent microscope wide-field system. The system include a workstation with 64GB DDR4 RAM, 11GB of graphic card, 512 gb of memory and SSD-RAID of 2TB. There is an option to increase the SSD-RAID to 4TB for about 6000$ extra. The system will be use for both fixed materiel imaging and for live-imaging with time-laps, z-stack, tiling and multi-fluorophores experiments. I was wondering if it is worth to invest those extra money for this option or not. This will be the first time we will have time-laps experiments, so I don't know what to expect as pictures size.
>>
>> What do you think?
>>
>> Thanks in advance for your comments and opinions!
>>

--
Ralf Palmisano
Head - Optical Imaging Centre Erlangen

Fellow Royal Microscopical Society
Member Royal Society of Medicine

Speaker Scientific Advisory Board "German Society for Microscopy and Image Analysis"
Board of Directors "Core Technologies for Life Sciences"

Cauerstr. 3
91058 Erlangen

[phone] (Secretary)
[phone] (Office)

[Pat Robison]

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Hi Julia,

That's maybe a better question than you are thinking, certainly not one
with a single answer.  It depends very much on what you want to show.  I
see flattened z-stacks all the time, which is ok for relatively simple
images where you are using a confocal/single plane type system to mitigate
out of focus light, but depth information is not important.  If you have a
single channel, depth coding can be a very effective presentation (there is
a button in zen, you may have to dig for a macro in fiji; keep in mind also
this requires your audience to have good color perception, which not
everyone does).  If depth is important and you want to compare 2+ channels,
you can split your z (or t) into substacks and combine them edgewise into
an image array so that they play simultaneously, but it becomes hard to
display so it can compromise resolution.

-Pat Robison

On Sat, May 25, 2019, 3:49 AM Julia Edgar <[hidden email] wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All
> A very naive question I know...
>
> How do you display time-lapse combined with confocal z stack? Flatten the
> z stack and play the time-lapse movie of the flattened (maximum intensity
> projection?) images?
>
> If so, how do you generate the movie using Zen software and/or Fiji?
>
> Many thanks in advance.
> Julia
>
> Get Outlook for iOS<https://aka.ms/o0ukef>
>
> ________________________________
> From: Confocal Microscopy List <[hidden email]> on
> behalf of Ralf Palmisano <[hidden email]>
> Sent: Saturday, May 25, 2019 1:47 am
> To: [hidden email]
> Subject: Re: SSD-RAID for new wide-field microscopy system
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Mirco,
>
> I am literally signing everything Nuno Mureno wrote. In addition whoever
> is the manufatcurer. I consider it bad advise to invest in an 11 GB
> graphics card and even worse to call 6.000$ for a 2 TB SSD extension. That
> is just playing foul or they have no clue about what they are selling and
> why...
>
> My pennie in here
>
> Ralph
>
> Am 23/05/2019 um 14:57 schrieb Nuno Moreno:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi Mirco
> >
> > Honestly I see no reason to have so much memory and such a high spec
> graphics card for an acquisition computer. Regarding the HDs, I would keep
> the 2TB and add extra space non SSD. Then just automate data flow from the
> fast to the slow drive. 2TB extra for 6000$ is just too much and might be
> too short anyway.
> >
> > Just my 2 cents
> > \N
> >
> >
> >
> >> On 23 May 2019, at 14:38, Mirco Martino <[hidden email]> wrote:
> >>
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> Post images on http://www.imgur.com and include the link in your
> posting.
> >> *****
> >>
> >> Hi all,
> >>
> >> we are buying a new fluorescent microscope wide-field system. The
> system include a workstation with 64GB DDR4 RAM, 11GB of graphic card, 512
> gb of memory and SSD-RAID of 2TB. There is an option to increase the
> SSD-RAID to 4TB for about 6000$ extra. The system will be use for both
> fixed materiel imaging and for live-imaging with time-laps, z-stack, tiling
> and multi-fluorophores experiments. I was wondering if it is worth to
> invest those extra money for this option or not. This will be the first
> time we will have time-laps experiments, so I don't know what to expect as
> pictures size.
> >>
> >> What do you think?
> >>
> >> Thanks in advance for your comments and opinions!
> >>
> --
> Ralf Palmisano
> Head - Optical Imaging Centre Erlangen
>
> Fellow Royal Microscopical Society
> Member Royal Society of Medicine
>
> Speaker Scientific Advisory Board "German Society for Microscopy and Image
> Analysis"
> Board of Directors "Core Technologies for Life Sciences"
>
> Cauerstr. 3
> 91058 Erlangen
>
> [phone] (Secretary)
> [phone] (Office)
>

[Tim Feinstein]

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*****

As a rule I try to keep the analysis and the presentation as straightforward as possible by reducing dimensions and cropping. If I have to include depth, time, and color then I usually generate a volume projection and have it rotate slowly while advancing in time. Most proprietary scope software is good at this, though you often have to pay a substantial extra license for the volume/movie option. ImageJ/Fiji is not ideal but I  found Fluorender and Vaa3D to be good free alternatives.

Bear in mind that volume rendering involves a lot of semi-arbitrary and nonlinear transformations.  So that sort of display is best if it can supplement quantitative analysis rather than stand on its own.

Best,


T

Timothy Feinstein, Ph.D.

> On May 25, 2019, at 3:49 AM, Julia Edgar <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://nam05.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Ctnf8%40PITT.EDU%7C37217e5d7f2d4da10e6d08d6e0e5752b%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C636943673473526194&amp;sdata=5GSXUi5wI5VV95zWAuLJdb7YE13pvsU8HJ9fB9B5sM4%3D&amp;reserved=0
> Post images on https://nam05.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Ctnf8%40PITT.EDU%7C37217e5d7f2d4da10e6d08d6e0e5752b%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C636943673473526194&amp;sdata=kRiTixuJ4TypED3sqISbUldlF5gWId2UiBxpAJwsdaU%3D&amp;reserved=0 and include the link in your posting.
> *****
>
> Dear All
> A very naive question I know...
>
> How do you display time-lapse combined with confocal z stack? Flatten the z stack and play the time-lapse movie of the flattened (maximum intensity projection?) images?
>
> If so, how do you generate the movie using Zen software and/or Fiji?
>
> Many thanks in advance.
> Julia
>
> Get Outlook for iOS<https://nam05.safelinks.protection.outlook.com/?url=https%3A%2F%2Faka.ms%2Fo0ukef&amp;data=02%7C01%7Ctnf8%40PITT.EDU%7C37217e5d7f2d4da10e6d08d6e0e5752b%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C636943673473526194&amp;sdata=LGBXuDrGU6PccKel94UFmyDp3dYKyREbCb1ZV0neyyA%3D&amp;reserved=0>
>
> ________________________________
> From: Confocal Microscopy List <[hidden email]> on behalf of Ralf Palmisano <[hidden email]>
> Sent: Saturday, May 25, 2019 1:47 am
> To: [hidden email]
> Subject: Re: SSD-RAID for new wide-field microscopy system
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://nam05.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Ctnf8%40PITT.EDU%7C37217e5d7f2d4da10e6d08d6e0e5752b%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C636943673473526194&amp;sdata=5GSXUi5wI5VV95zWAuLJdb7YE13pvsU8HJ9fB9B5sM4%3D&amp;reserved=0
> Post images on https://nam05.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Ctnf8%40PITT.EDU%7C37217e5d7f2d4da10e6d08d6e0e5752b%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C636943673473526194&amp;sdata=kRiTixuJ4TypED3sqISbUldlF5gWId2UiBxpAJwsdaU%3D&amp;reserved=0 and include the link in your posting.
> *****
>
> Hi Mirco,
>
> I am literally signing everything Nuno Mureno wrote. In addition whoever is the manufatcurer. I consider it bad advise to invest in an 11 GB graphics card and even worse to call 6.000$ for a 2 TB SSD extension. That is just playing foul or they have no clue about what they are selling and why...
>
> My pennie in here
>
> Ralph
>
>> Am 23/05/2019 um 14:57 schrieb Nuno Moreno:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> https://nam05.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Ctnf8%40PITT.EDU%7C37217e5d7f2d4da10e6d08d6e0e5752b%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C636943673473526194&amp;sdata=5GSXUi5wI5VV95zWAuLJdb7YE13pvsU8HJ9fB9B5sM4%3D&amp;reserved=0
>> Post images on https://nam05.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Ctnf8%40PITT.EDU%7C37217e5d7f2d4da10e6d08d6e0e5752b%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C636943673473526194&amp;sdata=kRiTixuJ4TypED3sqISbUldlF5gWId2UiBxpAJwsdaU%3D&amp;reserved=0 and include the link in your posting.
>> *****
>>
>> Hi Mirco
>>
>> Honestly I see no reason to have so much memory and such a high spec graphics card for an acquisition computer. Regarding the HDs, I would keep the 2TB and add extra space non SSD. Then just automate data flow from the fast to the slow drive. 2TB extra for 6000$ is just too much and might be too short anyway.
>>
>> Just my 2 cents
>> \N
>>
>>
>>
>>> On 23 May 2019, at 14:38, Mirco Martino <[hidden email]> wrote:
>>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> https://nam05.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Ctnf8%40PITT.EDU%7C37217e5d7f2d4da10e6d08d6e0e5752b%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C636943673473536187&amp;sdata=466LSBiLMjW2ge9y2Lhu3yISXXttR3R%2FHQmK4micXeQ%3D&amp;reserved=0
>>> Post images on https://nam05.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Ctnf8%40PITT.EDU%7C37217e5d7f2d4da10e6d08d6e0e5752b%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C636943673473536187&amp;sdata=47wjiIRXYriXmXI8TUK4m0Y74zV7uwKkek2qmQHD000%3D&amp;reserved=0 and include the link in your posting.
>>> *****
>>>
>>> Hi all,
>>>
>>> we are buying a new fluorescent microscope wide-field system. The system include a workstation with 64GB DDR4 RAM, 11GB of graphic card, 512 gb of memory and SSD-RAID of 2TB. There is an option to increase the SSD-RAID to 4TB for about 6000$ extra. The system will be use for both fixed materiel imaging and for live-imaging with time-laps, z-stack, tiling and multi-fluorophores experiments. I was wondering if it is worth to invest those extra money for this option or not. This will be the first time we will have time-laps experiments, so I don't know what to expect as pictures size.
>>>
>>> What do you think?
>>>
>>> Thanks in advance for your comments and opinions!
>>>
> --
> Ralf Palmisano
> Head - Optical Imaging Centre Erlangen
>
> Fellow Royal Microscopical Society
> Member Royal Society of Medicine
>
> Speaker Scientific Advisory Board "German Society for Microscopy and Image Analysis"
> Board of Directors "Core Technologies for Life Sciences"
>
> Cauerstr. 3
> 91058 Erlangen
>
> [phone] (Secretary)
> [phone] (Office)

[Vergara, Leoncio A.]

*****
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*****

You can do the time display using a projection or 3D rendering, or you can obviously select a single slice and display the time animation at that slice. One problem of time displaying a single slice is that your sample may “wonder” in z during the time lapse.

Sent from my iPhone

> On May 25, 2019, at 2:49 AM, Julia Edgar <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIFAg&c=cpvmSBWXd8YiHoMtYk_a9E2QIiaEheG3-gfMB16YPq0&r=q_wjVHQNtuyPtW0pWZ8VUASIgPYhLzAdCNyJUslsVzo&m=c0HBjYo7EdWDu130s9fC9Swb8BEnTR-DiEy5AHodCY8&s=MNp04voV1E36DmvjkuOvQXs7WLyt6W9tZRYTJwCgWRw&e=
> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIFAg&c=cpvmSBWXd8YiHoMtYk_a9E2QIiaEheG3-gfMB16YPq0&r=q_wjVHQNtuyPtW0pWZ8VUASIgPYhLzAdCNyJUslsVzo&m=c0HBjYo7EdWDu130s9fC9Swb8BEnTR-DiEy5AHodCY8&s=inpxQGvhaj0MvS1K7Q5e2ioOxVe4wDgHsbwnkZ9aHQs&e= and include the link in your posting.
> *****
>
> Dear All
> A very naive question I know...
>
> How do you display time-lapse combined with confocal z stack? Flatten the z stack and play the time-lapse movie of the flattened (maximum intensity projection?) images?
>
> If so, how do you generate the movie using Zen software and/or Fiji?
>
> Many thanks in advance.
> Julia
>
> Get Outlook for iOS<https://urldefense.proofpoint.com/v2/url?u=https-3A__aka.ms_o0ukef&d=DwIFAg&c=cpvmSBWXd8YiHoMtYk_a9E2QIiaEheG3-gfMB16YPq0&r=q_wjVHQNtuyPtW0pWZ8VUASIgPYhLzAdCNyJUslsVzo&m=c0HBjYo7EdWDu130s9fC9Swb8BEnTR-DiEy5AHodCY8&s=UbBQRFkhmfkFyODw6h8ZXcKRvIJNtntmT8kJPqqSO-g&e=>
>
> ________________________________
> From: Confocal Microscopy List <[hidden email]> on behalf of Ralf Palmisano <[hidden email]>
> Sent: Saturday, May 25, 2019 1:47 am
> To: [hidden email]
> Subject: Re: SSD-RAID for new wide-field microscopy system
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIFAg&c=cpvmSBWXd8YiHoMtYk_a9E2QIiaEheG3-gfMB16YPq0&r=q_wjVHQNtuyPtW0pWZ8VUASIgPYhLzAdCNyJUslsVzo&m=c0HBjYo7EdWDu130s9fC9Swb8BEnTR-DiEy5AHodCY8&s=MNp04voV1E36DmvjkuOvQXs7WLyt6W9tZRYTJwCgWRw&e=
> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIFAg&c=cpvmSBWXd8YiHoMtYk_a9E2QIiaEheG3-gfMB16YPq0&r=q_wjVHQNtuyPtW0pWZ8VUASIgPYhLzAdCNyJUslsVzo&m=c0HBjYo7EdWDu130s9fC9Swb8BEnTR-DiEy5AHodCY8&s=inpxQGvhaj0MvS1K7Q5e2ioOxVe4wDgHsbwnkZ9aHQs&e= and include the link in your posting.
> *****
>
> Hi Mirco,
>
> I am literally signing everything Nuno Mureno wrote. In addition whoever is the manufatcurer. I consider it bad advise to invest in an 11 GB graphics card and even worse to call 6.000$ for a 2 TB SSD extension. That is just playing foul or they have no clue about what they are selling and why...
>
> My pennie in here
>
> Ralph
>
>> Am 23/05/2019 um 14:57 schrieb Nuno Moreno:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>> *****
>>
>> Hi Mirco
>>
>> Honestly I see no reason to have so much memory and such a high spec graphics card for an acquisition computer. Regarding the HDs, I would keep the 2TB and add extra space non SSD. Then just automate data flow from the fast to the slow drive. 2TB extra for 6000$ is just too much and might be too short anyway.
>>
>> Just my 2 cents
>> \N
>>
>>
>>
>>> On 23 May 2019, at 14:38, Mirco Martino <[hidden email]> wrote:
>>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIFAg&c=cpvmSBWXd8YiHoMtYk_a9E2QIiaEheG3-gfMB16YPq0&r=q_wjVHQNtuyPtW0pWZ8VUASIgPYhLzAdCNyJUslsVzo&m=c0HBjYo7EdWDu130s9fC9Swb8BEnTR-DiEy5AHodCY8&s=MNp04voV1E36DmvjkuOvQXs7WLyt6W9tZRYTJwCgWRw&e=
>>> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIFAg&c=cpvmSBWXd8YiHoMtYk_a9E2QIiaEheG3-gfMB16YPq0&r=q_wjVHQNtuyPtW0pWZ8VUASIgPYhLzAdCNyJUslsVzo&m=c0HBjYo7EdWDu130s9fC9Swb8BEnTR-DiEy5AHodCY8&s=inpxQGvhaj0MvS1K7Q5e2ioOxVe4wDgHsbwnkZ9aHQs&e= and include the link in your posting.
>>> *****
>>>
>>> Hi all,
>>>
>>> we are buying a new fluorescent microscope wide-field system. The system include a workstation with 64GB DDR4 RAM, 11GB of graphic card, 512 gb of memory and SSD-RAID of 2TB. There is an option to increase the SSD-RAID to 4TB for about 6000$ extra. The system will be use for both fixed materiel imaging and for live-imaging with time-laps, z-stack, tiling and multi-fluorophores experiments. I was wondering if it is worth to invest those extra money for this option or not. This will be the first time we will have time-laps experiments, so I don't know what to expect as pictures size.
>>>
>>> What do you think?
>>>
>>> Thanks in advance for your comments and opinions!
>>>
> --
> Ralf Palmisano
> Head - Optical Imaging Centre Erlangen
>
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