STED slides

[Dun, Alison]

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Hi

Looking for some training slides for the SP8 3X STED we have just had installed. Have 775 and 592 lasers and want some multicolour prepared slides for training and for media images etc. Anyone know of where I can get some? Leica are low in stock for theirs so need alternative ideas,

Cheers!
Ali


Dr. Alison Dun
Edinburgh Super Resolution Imaging Consortium (ESRIC)
3.29 William Perkin Building
Heriot-Watt University
Edinburgh
EH14 4AS
Office: [phone]
Mobile: [phone]

http://www.esric.org
Follow us on Twitter @ESRIC_Imaging

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[Cole, Richard W (HEALTH)]

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Any slide containing labeled microtubules and/or actin (avoid Hoechst or Dapi) will demonstrate the STED effect very nicely.  They are very easy to make yourself.

Example: https://drive.google.com/drive/folders/1-KwjiBNi2wY3nd6aSs10cRs6oM6GcLv7?usp=sharing

Rich

Richard Cole
Research Scientist V
Director: Advanced Light Microscopy & Image Analysis Core
Wadsworth Center
 
Research Assistant Professor
Dept. of Biomedical Sciences
School of Public Health State University of New York

120 New Scotland Avenue, Albany N.Y. 12208
518-474-7048 Phone
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Subject: CONFOCALMICROSCOPY Digest - 14 Mar 2018 to 15 Mar 2018 (#2018-66)

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CONFOCALMICROSCOPY Digest - 14 Mar 2018 to 15 Mar 2018 (#2018-66)
Table of contents:
. STED slides
. Montreal Light Microscopy Course (MLMC) 2018, Montreal, July 16-20, 2018
. frame averaging with micromanager
. Dispersion measurement service (2)
. Filtered into Junk
. Macro to color Zeiss confocal lambda/spectral stacks
1. STED slides
o cid:[hidden email] (03/15)
From: "Dun, Alison" <mailto:[hidden email]>
2. Montreal Light Microscopy Course (MLMC) 2018, Montreal, July 16-20, 2018
o cid:[hidden email] (03/15)
From: "Claire Brown, Dr." <mailto:[hidden email]>
3. frame averaging with micromanager
o cid:[hidden email] (03/15)
From: "Owens, Peter" <mailto:[hidden email]>
4. Dispersion measurement service
o cid:[hidden email] (03/15)
From: Adam Glaser <mailto:[hidden email]>
o cid:[hidden email] (03/15)
From: "MODEL, MICHAEL" <mailto:[hidden email]>
5. Filtered into Junk
o cid:[hidden email] (03/15)
From: Claire Brown <mailto:[hidden email]>
6. Macro to color Zeiss confocal lambda/spectral stacks
o cid:[hidden email] (03/15)
From: "G. Esteban Fernandez" <mailto:[hidden email]>


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[Steffen Dietzel]

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Hi Ali,

what we use for courses and introductions are two color slides:
Alexa594/Atto594/Aberrior Star 580 in one channel and Atto647N/Aberrior
Star 635P/SiR in the other. For example, nuclear pores and actin are a
nice pair of targets. Other than using these dyes (which are less prone
to bleaching under STED conditions than other dyes) this is a normal
immunostaining, so anyone with a little experience, a running cell
culture, and the reagents should be able to do it. EdU replication
labeling also looks cool, but doesn't work together with actin staining.
When I see that Molecular Probes sells slides between 200 and 350 Euro a
piece (not STED slides, I think), it seems that buying the reagents
comes cheaper very quickly.

We usually embed in ProLong Diamond. I am not really happy about it,
because it shrinks the sample along the z-axis when it cures and it
doesn't seem particular intelligent to first shrink and then do
superresolution. But it does provide nice anti-fade properties.

Compared to the 775, we had much less convincing results with the 592. I
hear it is good for YFP, but we didn't try that one ourselves.

Have fun with your new scope!

Steffen


Am 15.03.2018 um 09:45 schrieb Dun, Alison:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi
>
> Looking for some training slides for the SP8 3X STED we have just had installed. Have 775 and 592 lasers and want some multicolour prepared slides for training and for media images etc. Anyone know of where I can get some? Leica are low in stock for theirs so need alternative ideas,
>
> Cheers!
> Ali
>
>
> Dr. Alison Dun
> Edinburgh Super Resolution Imaging Consortium (ESRIC)
> 3.29 William Perkin Building
> Heriot-Watt University
> Edinburgh
> EH14 4AS
> Office: [phone]
> Mobile: [phone]
>
> http://www.esric.org
> Follow us on Twitter @ESRIC_Imaging
>
> ________________________________
>
>

--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de