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Pascal,
I would suggest checking with Molecular Probes on the stability of
their of their various beads. They do make on series of beads that
come in a range of intensities that I have seen used as an intensity
standard. You build a curve characterizing your instrument using all
if the beads then spike your sample with one intensity of bead. This
then allows you to find the beads based on shape.
Chris Tully, M.S., Image Analysis Expert
t 240.475.9753 f 419.831.0527 |
[hidden email]
Sent from my iPhone please excuse typos.
On Jun 25, 2013, at 8:49 AM, Pascal Weber <
[hidden email]> wrote:
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>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hi everyone,
>
> I make GFP staining on bacteria in lice. I use it for the deconvolution spectral to
> discriminate autofluorescence in fluorescence. Now I would like to quantify the
> signal ! Does anyone have any idea how to achieve a standard reference and
> keep it over time ?
>
> For now I use the best signal that I could find to create this reference but is valid
> spectral deconvolution?
>
> Regards Pascal
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