Sealing the specimen mounted in cargille oils
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Shalin Mehta |
Sealing the specimen mounted in cargille oils
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Hello listers,
I am using Cargille immersion oil for mounting some test targets. I am looking for a good sealant that does not react with cargille immersion oils. Nail polish tends to mix with oils as it dries slowly. I am trying to avoid VALAP because it spreads on the coverslip if scratched accidentally. Is there something that will dry fast and not mix too much with the immersion oils? Thanks for suggestions Shalin blog: shalin.wordpress.com (Current:) Visiting Researcher at Oldenbourg Lab, Marine Biological Laboratory. Lillie 110, 7 MBL Street, Woods Hole, MA 02543, USA. (Permanent:) Bioimaging Lab, Block-E3A, #7-10 Div of Bioengineering, NUS Singapore 117574 website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/people.asp#shalinm |
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I use dental wax. Since we are an EM lab as well as optical we
always have some on a hotplate. It sets pretty much instantly as it cools and does
not seem to harm even the most delicate tissue.
Guy Please help fight breast cancer by sponsoring my run in the Sydney Half Marathon on May 16th. http://www.everydayhero.com.au/guy_cox ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Shalin Mehta Hello listers, No virus
found in this incoming message. |
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EricMarino |
Re: Sealing the specimen mounted in cargille oils
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In reply to this post by Shalin Mehta
Have you tried Mowiol? Eric Marino Cell: (617) 913-9647 Lab: (617) 713-8885 Sent from my iPhone
Eric Marino
Senior Imaging Specialist Program in Cellular and Molecular Medicine Boston Children's Hospital |
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COETZEE, S.H. (MR.) |
Re: Sealing the specimen mounted in cargille oils
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I am not sure how well it will work but we use a quick solution
of nail polish. Dries fast, seals well and surprising some slides are OK after
two years. Best of luck. From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of Eric Marino Have you tried Mowiol? Cell: (617) 913-9647 Lab: (617) 713-8885 Sent from my iPhone
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McDonald, David L |
Re: Sealing the specimen mounted in cargille oils
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In reply to this post by Shalin Mehta
In the past I've used rubber cement with success for other samples.
Never tried using it with immersion oil as a mounting medium
though....
Dave Dave McDonald Scientific Imaging Lab Fred Hutchinson Cancer Research Center 1100 Fairview Avenue North, DE-512 Seattle, WA 98109 206-667-4205 http://www.fhcrc.org At 08:22 PM 5/22/2010, you wrote: Hello listers, |
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Glen MacDonald-2 |
Re: Sealing the specimen mounted in cargille oils
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Silicone grease may work as a last resort, but using it around lenses always gives me the willies.
Regards,Glen On May 25, 2010, at 10:23 AM, Dave McDonald wrote: > In the past I've used rubber cement with success for other samples. Never tried using it with immersion oil as a mounting medium though.... > > Dave > > Dave McDonald > Scientific Imaging Lab > Fred Hutchinson Cancer Research Center > 1100 Fairview Avenue North, DE-512 > Seattle, WA 98109 > 206-667-4205 > http://www.fhcrc.org > > At 08:22 PM 5/22/2010, you wrote: >> Hello listers, >> I am using Cargille immersion oil for mounting some test targets. I am looking for a good sealant that does not react with cargille immersion oils. Nail polish tends to mix with oils as it dries slowly. I am trying to avoid VALAP because it spreads on the coverslip if scratched accidentally. Is there something that will dry fast and not mix too much with the immersion oils? >> >> Thanks for suggestions >> >> Shalin >> blog: shalin.wordpress.com >> >> (Current:) Visiting Researcher at Oldenbourg Lab, Marine Biological Laboratory. >> Lillie 110, 7 MBL Street, Woods Hole, MA 02543, USA. >> >> (Permanent:) >> Bioimaging Lab, Block-E3A, #7-10 >> Div of Bioengineering, NUS Singapore 117574 >> website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/people.asp#shalinm Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 [hidden email] |
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James Denegre |
Re: Sealing the specimen mounted in cargille oils
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Jim On 5/25/10 1:30 PM, "Glen MacDonald" <glenmac@...> wrote: Silicone grease may work as a last resort, but using it around lenses always gives me the willies. |
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