Searching for Olympus IX2 part (right side port adaptor unit, IX2-RSPC)

[Abin Biswas]

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Hi everyone

We are planning to build a custom quantitative phase + confocal microscope on an old IX-81 frame.
In addition to the standard image acquisition via the left side port, we would also like to access the primary image via the right-side port.

Does anyone have a spare IX2-RSPC unit that they would be willing to lend or share with us? Or would anyone know where I could buy one from? We have already contacted Olympus and the part is no longer in stock/available and I could not find anything on eBay.

Additionally, would anyone have a suggestion on how to access the image from the right-side port without dismantling the microscope completely?

Thanks a lot for your help.

Best regards
Abin Biswas
Postdoc
Guck division
Max Planck Institute for the Science of Light

[George McNamara]

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Hi Abin,

if no one has hat you want, I suggest contacting Rapp Opto-Electronic, see

https://rapp-opto.com/products/microscope-devices-accessories/optical-couplings/ix3-breadboard/

and elsewhere on their web site, but also contact them directly.

George

p.s. my local Olympus rep introduced me to Rapp by showing me their web
page (may be discontinued) of a custom Olympus upright + inverted
microscope (yes, 2 sets of eyepieces).


On 4/27/2020 12:18 PM, Abin Biswas wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi everyone
>
> We are planning to build a custom quantitative phase + confocal microscope on an old IX-81 frame.
> In addition to the standard image acquisition via the left side port, we would also like to access the primary image via the right-side port.
>
> Does anyone have a spare IX2-RSPC unit that they would be willing to lend or share with us? Or would anyone know where I could buy one from? We have already contacted Olympus and the part is no longer in stock/available and I could not find anything on eBay.
>
> Additionally, would anyone have a suggestion on how to access the image from the right-side port without dismantling the microscope completely?
>
> Thanks a lot for your help.
>
> Best regards
> Abin Biswas
> Postdoc
> Guck division
> Max Planck Institute for the Science of Light

[Michael Shribak]

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*****

Hi Abin,

You may wish to consider to use the bottom port, which creates a direct primary image without any reflections and any optical components, and the quantitative DIC:

https://www.researchgate.net/publication/322582062_An_orientation-independent_DIC_microscope_allows_high_resolution_imaging_of_epithelial_cell_migration_and_wound_healing_in_a_Cnidarian_model

Michael Shribak

Marine Biological Laboratory
7 MBL St
Woods Hole, MA 02543





On Apr 27, 2020, at 12:55:12 PM, George McNamara <[hidden email]> wrote:

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*****

Hi Abin,

if no one has hat you want, I suggest contacting Rapp Opto-Electronic, see

https://rapp-opto.com/products/microscope-devices-accessories/optical-couplings/ix3-breadboard/

and elsewhere on their web site, but also contact them directly.

George

p.s. my local Olympus rep introduced me to Rapp by showing me their web page (may be discontinued) of a custom Olympus upright + inverted microscope (yes, 2 sets of eyepieces).


On 4/27/2020 12:18 PM, Abin Biswas wrote:
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi everyone

We are planning to build a custom quantitative phase + confocal microscope on an old IX-81 frame.
In addition to the standard image acquisition via the left side port, we would also like to access the primary image via the right-side port.

Does anyone have a spare IX2-RSPC unit that they would be willing to lend or share with us? Or would anyone know where I could buy one from? We have already contacted Olympus and the part is no longer in stock/available and I could not find anything on eBay.

Additionally, would anyone have a suggestion on how to access the image from the right-side port without dismantling the microscope completely?

Thanks a lot for your help.

Best regards
Abin Biswas
Postdoc
Guck division
Max Planck Institute for the Science of Light

[Craig Brideau]

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*****

Michael is right. Ideally you want as few optical elements as possible in
the light path when using phase methods. Each surface can (and probably
will) introduce phase distortions into the wave and surface flatness
becomes critical (more so than usual).

Craig

On Mon, Apr 27, 2020 at 11:56 AM Michael Shribak <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Abin,
>
> You may wish to consider to use the bottom port, which creates a direct
> primary image without any reflections and any optical components, and the
> quantitative DIC:
>
>
> https://www.researchgate.net/publication/322582062_An_orientation-independent_DIC_microscope_allows_high_resolution_imaging_of_epithelial_cell_migration_and_wound_healing_in_a_Cnidarian_model
>
> Michael Shribak
>
> Marine Biological Laboratory
> 7 MBL St
> Woods Hole, MA 02543
>
>
>
>
>
> On Apr 27, 2020, at 12:55:12 PM, George McNamara <
> [hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Abin,
>
> if no one has hat you want, I suggest contacting Rapp Opto-Electronic, see
>
>
> https://rapp-opto.com/products/microscope-devices-accessories/optical-couplings/ix3-breadboard/
>
> and elsewhere on their web site, but also contact them directly.
>
> George
>
> p.s. my local Olympus rep introduced me to Rapp by showing me their web
> page (may be discontinued) of a custom Olympus upright + inverted
> microscope (yes, 2 sets of eyepieces).
>
>
> On 4/27/2020 12:18 PM, Abin Biswas wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi everyone
>
> We are planning to build a custom quantitative phase + confocal microscope
> on an old IX-81 frame.
> In addition to the standard image acquisition via the left side port, we
> would also like to access the primary image via the right-side port.
>
> Does anyone have a spare IX2-RSPC unit that they would be willing to lend
> or share with us? Or would anyone know where I could buy one from? We have
> already contacted Olympus and the part is no longer in stock/available and
> I could not find anything on eBay.
>
> Additionally, would anyone have a suggestion on how to access the image
> from the right-side port without dismantling the microscope completely?
>
> Thanks a lot for your help.
>
> Best regards
> Abin Biswas
> Postdoc
> Guck division
> Max Planck Institute for the Science of Light
>

[Abin Biswas]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****


@George
Thank you for the suggestion. I've actually been eyeing the Rapp website for a while and I emailed them today. Hoping they have some solution or the exact part we need.


@Michael
Thank you for replying and the suggestion! We've been playing around with the idea of using the bottom port as we have an optical table with a hole cut out for accessing the bottom port as well.

However, we plan to use Optical Diffraction Tomography for our phase imaging:


https://arxiv.org/pdf/1603.00592.pdf


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2832333/


and, would thus need to interfere our sample and reference beams under the optical table. This is one of the reasons why we would like to access the right side port.


Additionally what you and @Craig mentioned regarding surface flatness is indeed critical and we will need to take that into account.


Best

Abin


________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Craig Brideau <[hidden email]>
Sent: Monday, April 27, 2020 8:17:04 PM
To: [hidden email]
Subject: Re: Searching for Olympus IX2 part (right side port adaptor unit, IX2-RSPC)

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Michael is right. Ideally you want as few optical elements as possible in
the light path when using phase methods. Each surface can (and probably
will) introduce phase distortions into the wave and surface flatness
becomes critical (more so than usual).

Craig

On Mon, Apr 27, 2020 at 11:56 AM Michael Shribak <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Abin,
>
> You may wish to consider to use the bottom port, which creates a direct
> primary image without any reflections and any optical components, and the
> quantitative DIC:
>
>
> https://www.researchgate.net/publication/322582062_An_orientation-independent_DIC_microscope_allows_high_resolution_imaging_of_epithelial_cell_migration_and_wound_healing_in_a_Cnidarian_model
>
> Michael Shribak
>
> Marine Biological Laboratory
> 7 MBL St
> Woods Hole, MA 02543
>
>
>
>
>
> On Apr 27, 2020, at 12:55:12 PM, George McNamara <
> [hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Abin,
>
> if no one has hat you want, I suggest contacting Rapp Opto-Electronic, see
>
>
> https://rapp-opto.com/products/microscope-devices-accessories/optical-couplings/ix3-breadboard/
>
> and elsewhere on their web site, but also contact them directly.
>
> George
>
> p.s. my local Olympus rep introduced me to Rapp by showing me their web
> page (may be discontinued) of a custom Olympus upright + inverted
> microscope (yes, 2 sets of eyepieces).
>
>
> On 4/27/2020 12:18 PM, Abin Biswas wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi everyone
>
> We are planning to build a custom quantitative phase + confocal microscope
> on an old IX-81 frame.
> In addition to the standard image acquisition via the left side port, we
> would also like to access the primary image via the right-side port.
>
> Does anyone have a spare IX2-RSPC unit that they would be willing to lend
> or share with us? Or would anyone know where I could buy one from? We have
> already contacted Olympus and the part is no longer in stock/available and
> I could not find anything on eBay.
>
> Additionally, would anyone have a suggestion on how to access the image
> from the right-side port without dismantling the microscope completely?
>
> Thanks a lot for your help.
>
> Best regards
> Abin Biswas
> Postdoc
> Guck division
> Max Planck Institute for the Science of Light
>