Secondary antibody for 405nm laser line ?

[R. Eric King]

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Hello Jerry -

How much "as is" ? We have the diodes in stock, and can repair these ;-)

Eric King

----- Original Message -----
From: "Jerry (Gerald) Sedgewick" <[hidden email]>
To: <[hidden email]>
Sent: Thursday, March 06, 2008 6:30 AM
Subject: Re: Tsunami for sale

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> We have aTi:Sapphire laser system we are interested in selling.  This is
> the mode-locked Ti:Sapphire laser (Tsunami 3960, Spectra Physics Inc.,
> Mountain View, CA) pumped by a high-power (5 W, 532 nm) laser (Millennia
> V, Spectra Physics Inc.). It comes with a spectrometer.  One of the diode
> lasers used in the Millenia is not operational: if you're interested, then
> we'd have that repaired.
> If this is of interest to you, please make an offer with the idea that the
> Millennia is repaired and in prime operating condition.
> Send email to:  Jerry Sedgewick: [hidden email].
>
> Thanks!
>
> Jerry Sedgewick
> Program Director, Biomedical Image Processing Lab
> University of Minnesota
>
> ÐÏࡱá
>
>
>
>
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[Michael Weber-4]

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Glen,

but what do you do if you have 4 or 5 stainings in your sample - you
have to involve blue. Or you select a green look-up-table for Alexa 405.
Or for quantitative approaches, one can use spectrum or fire lut.
Fiddling with the histogram is not always a good idea, at least when it
has to be scientific.

Michael


Glen MacDonald wrote:

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> All of the Alexa fluor conjugates have a tendency to stick
> non-specifically via charge interactions of the anionic dye moiety.  the
> Image-iT fx does helps greatly, especially in thick samples.
>
> In regard to using the blue fluorophores for the more prominent targets
> in the tissue, the human visual system is only weakly responsive to blue
> wavelengths and lacks blue-sensitive cones in the fovea, the region of
> greatest visual acuity.  In general, if the smallest or rarest bits get
> the green, red or gray channel, they are noticeable with less histogram
> manipulation than if the smallest bits are viewed in the blue channel.
>
>    Regards,
> Glen
>
>
>
> Glen MacDonald
> Core for Communication Research
> Virginia Merrill Bloedel Hearing Research Center
> Box 357923
> University of Washington
> Seattle, WA 98195-7923  USA
> [phone]
> [hidden email]
>
> ******************************************************************************
>
> The box said "Requires WindowsXP or better", so I bought a Macintosh.
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>
>
> On Mar 6, 2008, at 7:09 AM, Junya HIROI wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Christophe,
>>
>> I was also looking for a fluorescent dye for 405 nm excitation, and I
>> compared Alexa 405
>> with Pacific Blue.  Both dyes bleached to some extent with
>> ProlongGold, but Pacific Blue was
>> absolutely brighter than Alexa 405, when compared at the same
>> dilution.  So, I recommend
>> Pacific Blue, although Molecular Probes sells Pacific Blue for flow
>> cytometry.      
>>
>> I found another problem on Alexa 405: it nonspecifically binds to
>> nuclei !  I asked Molecular
>> Probes/Invitrogen about this problem, and they recommended me to use
>> their blocking
>> solution, Image-iT FX Signal Enhancer.  It actually worked well, but
>> Pacific Blue does not have
>> such a problem, so that is another reason I like Pacific Blue.
>>
>> Junya
>>
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