Hi all, I have never cut hard woody grass stems (similar strength as
small dried bamboo stem). One of our client wish to section and stain
them and I have tried couple of ways soak and cryo embedding, cook
(pressure cook) and mounting in paraffin (based on a book chapter), but
it seems not working well. I am about to try embedding in hard polymer
such as LR white or Lowacryl, is there any other method proven to be
useful than these approaches.
Sorry this is not a confocal question.
Thanking you in advance
Shiv
Mayandi Sivaguru, PhD, PhD
Microscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA
Office: 217.333.1214
Fax: 217.244.2496
[hidden email]
http://core.igb.uiuc.edu
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