One of the best ways I know to do this is described in our paper:
Quantitative measurement of the resolution and sensitivity of confocal microscopes using line-scanning fluorescence correlation spectroscopy
Microscopy Research and Technique
Volume 66, Issue 4, Date: 1 March 2005, Pages: 198-202
J. Balaji, S. Maiti
It may sound a bit daunting to the uninitiated, but it is pretty simple, really. All it takes is to record an image of a bead solution for a few seconds. The data can be analyzed with a program that I can send off the list.
Sudipta
On Thu, 2 Oct 2008 12:02:49 -0700, Ken Pluomis wrote
> Dear all,
>
>
>
> We are in the process of purchasing a confocal microscope to serve a broad range of biological applications. We are considering the Leica SP5 and the Zeiss 710. Both companies are claiming [WINDOWS-1252?]our confocal provides the highest [WINDOWS-1252?]sensitivity, but they are unable to provide some proofs of that. Could you provide me some practical feedback?
>
> Particularly we are a bit confused about the AOBS. Leica people claim more than 95% transmittance, while Zeiss people are saying that the transmittance is much worse than any conventional beam splitter. Again nobody is providing any kind of evidence of such statements.. Do you have any experience/information on this subject?
>
> Thanks.
>
> Dr. Ken. G. Pluomis
Dr. Sudipta Maiti
Associate Professor
Dept. of Chemical Sciences
Tata Institute of Fundamental Research
Homi Bhabha Raod, Colaba, Mumbai 400005
Ph. 91-22-2278-2716 / 2539
Fax: 91-22-2280-4610
alternate e-mail: [hidden email]
url: www.tifr.res.in/~biophotonics
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