Commercial Vendor Response
Dear All,
just to clarify some aspects of sensitivity of the Leica TCS SP5 with AOBS
(Acousto Optical Beam Splitter):
Yes, the AOBS has a transmittance of approx. 95%. See a
measured transmission curve at:
http://www.visual-worlds.de/Transmission/Transmission_AOBS.htm
The key advantage of the AOBS in terms of sensitivity however is coming
from
the high transmittance combined with (up to 8) extremely sharp and
tunable
bands, that are used to couple the excitation laser lines into
the Confocal Leica TCS SP5.
This way, extra bandwidth for detecting fluorescence emission is provided,
further increasing the overall collection efficiency of the
system. This advantage is even more
prominent, the more excitation lines are
used simultaneously e.g. for multi-labelled live specimens.
Please feel free to contact me offline for any further questions
or clarification.
Best regards
Martin
--------------------------------------------------------
Martin Hoppe,
Ph.D.
Head of Market Management & Confocal Applications
Life Science
Division
Leica Microsystems CMS GmbH
Am Friedensplatz 3 | 68165 Mannheim
(Germany)
Phone : +49 621 7028 1100 | Fax : +49 621 7028 1180
Cell: +49
172 623 0409
Ken Pluomis
<[hidden email]>
Sent by: Confocal Microscopy List
<[hidden email]>
02.10.2008 20:55
Please respond to Confocal Microscopy List
|
To:
[hidden email]
cc:
(bcc: Confocal News/DEMAN/LMSCentral/Leica)
Subject:
Sensitivity and
AOBS |
Dear all,
We are in the process of
purchasing a confocal microscope to serve a broad range of biological
applications. We are considering the Leica SP5 and the Zeiss 710. Both companies
are claiming âour confocal provides the highest sensitivityâ, but they are
unable to provide some proofs of that. Could you provide me some practical
feedback? Particularly we are a bit confused about the AOBS. Leica people claim
more than 95% transmittance, while Zeiss people are saying that the
transmittance is much worse than any conventional beam splitter. Again nobody is
providing any kind of evidence of such statements. Do you have any
experience/information on this subject? Thanks. Dr. Ken. G. Pluomis
Locked
