Sensitized emission FRET / Biosensor with Red and Far-Red proteins

[lgelman]

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Hi everybody,

We would like to construct a FRET biosensor for intra-vital imaging with a Red donor and Far-Red Acceptor.
Our cells already express GFP and SNAP/Halo-tag technology not possible (brain imaging).

Has anyone experience with FRET in that range of the light spectrum?

Many thanks in advance for your advices,

Very best  regards,

Laurent.


Laurent Gelman
Facility for Advanced Imaging and Microscopy
Head Light Microscopy
Friedrich Miescher Institute for Biomedical Research
Maulbeerstrasse 66
4058 basel
Switzerland
[phone]

[mcammer]

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Perhaps this would help?
Shcherbakova DM, Cammer NC, Huisman TM, Verkhusha VV, Hodgson L.  Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol. 2018 May 4. doi: 10.1038/s41589-018-0070-z. PMID: 29728601
https://www.nature.com/articles/s41589-018-0044-1 

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016
[hidden email]  http://nyulmc.org/micros  http://microscopynotes.com/ 
Voice direct only, no text or messages:  1-914-309-3270 and 1-646-501-0567



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gelman, Laurent
Sent: Tuesday, July 24, 2018 11:37 AM
To: [hidden email]
Subject: Sensitized emission FRET / Biosensor with Red and Far-Red proteins


Hi everybody,

We would like to construct a FRET biosensor for intra-vital imaging with a Red donor and Far-Red Acceptor.
Our cells already express GFP and SNAP/Halo-tag technology not possible (brain imaging).

Has anyone experience with FRET in that range of the light spectrum?

Many thanks in advance for your advices,

Very best  regards,

Laurent.


Laurent Gelman
Facility for Advanced Imaging and Microscopy
Head Light Microscopy
Friedrich Miescher Institute for Biomedical Research
Maulbeerstrasse 66
4058 basel
Switzerland
[phone]

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