Spinning disk alignment
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I am wondering if anyone out there with a Yokogawa spinning disk system has attempted to optimize the alignment/positioning of the various components and worked out a good way to do it. We have recently installed a system which has in order a Nikon TI, Yokogawa CSU-X, Sutter filter wheel and camera. How critical is the positioning of the Yokogawa relative to the microscope? How do you optimize that? The position of the camera relative to the CSU seems simple in that you would optimize for a sharp image on the camera. What is a good sample to do this with? Any hints appreciated. Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax) |
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Please feel free to contact me off line and I will be happy to tell you what I can. Cheers Robin On 10/02/09 10:04 PM, "David Knecht ATT" <david.knecht@...> wrote: I am wondering if anyone out there with a Yokogawa spinning disk system has attempted to optimize the alignment/positioning of the various components and worked out a good way to do it. We have recently installed a system which has in order a Nikon TI, Yokogawa CSU-X, Sutter filter wheel and camera. How critical is the positioning of the Yokogawa relative to the microscope? How do you optimize that? The position of the camera relative to the CSU seems simple in that you would optimize for a sharp image on the camera. What is a good sample to do this with? Any hints appreciated. Thanks- Dave Quorum Technologies Inc. light at work 4673 Wellington Rd. #35 RR#6 Guelph, Ontario Canada N1H 6J3 Robin Battye, M.Sc., Ph.D. Vice President Sales and Development robin@... www.quorumtechnologies.com Cell: (647) 285-9998 Office: (519) 824-0854 Fax: (519) 824-5845 Focused on Making light work for us! |
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Hi to all, When I was a grad student with Ted Salmon,
we wrote a methods chapter on the spinning disk which describes various aspects
including aligning the head to the microscope, the consequences of changing NA
and Mag, and the nemesis of multipoint scanning, crosstalk. This will also
point you to the original papers about the yokogawa design (tandem spinning
disk). Paul S. Maddox, B. Moree, J.C. Canman, and
E. D. Salmon. 2003. A spinning disk confocal microscope system for rapid high
resolution, multimode, fluorescence speckle microscopy and GFP imaging in
living cells. In, Biophotonics, Parts, A and B (G. Marriot and I. Parker,
eds.), Methods in Enyzmology, Vol. 360, Academic Press, If people have trouble getting a copy,
email me and I can send you a pdf. I should add that the Prairie/Nikon swept
field confocal is now a real competitor for the yokogawa. The added
flexibility of changing the aperture should not be underestimated. I am happy
to offer more “personal” thoughts offline on the various benefits
of the two systems. Hope this helps answer some of the
questions re spinning disk. Paul Paul S. Maddox, PhD From: Hi Dr. Knecht, I am wondering if anyone out there
with a Yokogawa spinning disk system has attempted to optimize the
alignment/positioning of the various components and worked out a good way to do
it. We have recently installed a system which has in order a Nikon TI,
Yokogawa CSU-X, Sutter filter wheel and camera. How critical is the
positioning of the Yokogawa relative to the microscope? How do you
optimize that? The position of the camera relative to the CSU seems
simple in that you would optimize for a sharp image on the camera. What
is a good sample to do this with? Any hints appreciated. Thanks-
Dave Quorum
Technologies Inc. |
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