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Spot scan mode

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John Pediani

Spot scan mode

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Hi is it possible to set up spot scanning
mode using the Leica SP8. I am
trying to replicate Zeiss spot scanning
mode---time series.

Steffen Dietzel

Re: Spot scan mode

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John,

what exactly do you mean by spot scanning? Scanning with a spot
(excitation volume) is what a confocal does anyway.

If you mean parking the beam on a specific spot (i.e. *not* scanning),
you can do that. We use that when measuring the laser power. Therefore
the procedure is explained in the respective protocol on our web site:

http://www.bioimaging.bmc.med.uni-muenchen.de/manuals-protocols/maintenance_protocolls/laserpower/index.html

(sorry for the long URL)

Best

Steffen

Am 11.05.2017 um 00:13 schrieb John Pediani:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi is it possible to set up spot scanning
> mode using the Leica SP8. I am
> trying to replicate Zeiss spot scanning
> mode---time series.
>

--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de

John Pediani

Re: Spot scan mode

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To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks for the response Steffen, parking the beam not scanning is the set up we use with the Zeiss software in spot scan mode but could find spot scan setting within the Leica software

Sent from AOL Mobile Mail

-----Original Message-----
From: Steffen Dietzel <[hidden email]>
To: CONFOCALMICROSCOPY <[hidden email]>
Sent: Thu, May 11, 2017 10:44 AM
Subject: Re: Spot scan mode

*****
To join, leave or search the confocal microscopy listserv, go to:
<a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_blank">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a>
Post images on <a href="http://www.imgur.com" target="_blank">http://www.imgur.com</a> and include the link in your posting.
*****

John,

what exactly do you mean by spot scanning? Scanning with a spot
(excitation volume) is what a confocal does anyway.

If you mean parking the beam on a specific spot (i.e. *not* scanning),
you can do that. We use that when measuring the laser power. Therefore
the procedure is explained in the respective protocol on our web site:

<a href="http://www.bioimaging.bmc.med.uni-muenchen.de/manuals-protocols/maintenance_protocolls/laserpower/index.html" target="_blank">http://www.bioimaging.bmc.med.uni-muenchen.de/manuals-protocols/maintenance_protocolls/laserpower/index.html</a>

(sorry for the long URL)

Best

Steffen

Am 11.05.2017 um 00:13 schrieb John Pediani:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_blank">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a>
> Post images on <a href="http://www.imgur.com" target="_blank">http://www.imgur.com</a> and include the link in your posting.
> *****
>
> Hi is it possible to set up spot scanning
> mode using the Leica SP8. I am
> trying to replicate Zeiss spot scanning
> mode---time series.
>