Stage micrometers and diatoms
Locked
|
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Dear confocalists,
Is there a stage micrometer that works in both transmission and reflection mode- for measuring true magnification? I have diatom slide from Mr. Klaus Kemp with 8 diatoms that allow evaluation of resolution. Are some features on diatom frustule known to have certain distance between them? Following diatoms are mounted on the slide.
Shalin -- My co-ordinates: Shalin Mehta, Graduate student Graduate Programme in Bioengineering, NUS, Singapore Email: shalin {dot} mehta {at} gmail {dot} com Blog: electricsbm.blogspot.com Mobile: +65 90694182 |
 
|
Gary Laevsky-2 |
Re: Stage micrometers and diatoms
|
|
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
We used to use
chrome on glass micrometers from Applied Precision for just this purpose.
Works great! Best, Gary Laevsky, Ph.D. Imaging Application Specialist Andor Technology discover new ways of seeing Cell (774) 291 - 9992 Office (860) 290 - 9211 x219 Fax (860) 290 - 9566 Web: www.andor.com From: Confocal
Microscopy List [mailto:[hidden email]] On Behalf Of Shalin Mehta Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear confocalists,
Best, |
|
|
Barbara Foster |
Re: Stage micrometers and diatoms
|
|
In reply to this post by Shalin Mehta
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi, Shalin,
In answer to your various questions: a. A transmitted light micrometer that has been made using chrome on glass will work in both transmitted and reflected light b. Yes, there are features on diatoms that have specific spacing and Mr. Kemp most likely provided a reference sheet with your diatom slide. Mine is currently waiting to be unpacked, but I am sure some of the diatomists in the audience can provide those numbers off the top of their heads. This particular set of diatoms is was chosen to give a range of those spacings. If you can't get an answer, I'll contact you when I get my bench unpacked. Best regards, Barbara Barbara Foster, President We've moved! Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com MME is now scheduling customized, on-site courses through December. Call us today for details. P. S. Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Just call us here in the MME office for details. At 07:37 AM 8/10/2007, you wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear confocalists, |
|
|
Julio Vazquez |
Re: Stage micrometers and diatoms
|
|
In reply to this post by Shalin Mehta
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Dear diatom lovers,
below are the spacings of the features of the different species on Klaus Kemp's 8-form test plate. I believe I got this info from the Carolina Biological supply web site, when they used to sell the slide. There also used to be an article by Dave Walker: Counting teh dots: Giving Microscopes a workout using diatom test slides at www.microscopy-uk.org.uk/mag/artoct99/dwdiatom.html the article seems to be currently inaccessible, possibly being revised Julio.
-- Julio Vazquez Fred Hutchinson Cancer Research Center Seattle, WA 98109-1024 On Aug 10, 2007, at 5:37 AM, Shalin Mehta wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear confocalists, |
|
|
Julio Vazquez |
Re: Stage micrometers and diatoms
|
|
In reply to this post by Shalin Mehta
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
- Shalin, When we purchased our Zeiss confocal, we got several test slides from Zeiss; these include a grid pattern of 2 micron wide (If I recall correctly) lines at 100 micron spacing, and a grating of 0.5 micron lines at 1 micron spacing (alternating dark and light 0.5 micron lines). In both cases, the lines are made of opaque/reflective thin metal deposit, I believe. These work well for measuring magnification and resolution/contrast. I am not sure where Zeiss gets them, or whether they sell them independently of their confocals, but if you have a contact for a Zeiss rep, might be worth asking them... Another source of micrometers is Applied Image Inc... they cost around $ 400.00. I think they call them "scales" Carolina Biological Supply company has several conventional stage micrometers for about $ 30.00-50.00. Don't know if any of those work in reflected light... Google "Carolina Biological" , and then search for micrometer -- Julio Vazquez, Fred Hutchinson Cancer Research Center Seattle, WA 98109-1024 On Aug 10, 2007, at 5:37 AM, Shalin Mehta wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear confocalists, |
|
|
Steffen Dietzel |
Re: Stage micrometers and diatoms
|
|
In reply to this post by Shalin Mehta
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal At 14:37 10.08.2007, you wrote: > Is there a stage micrometer that works in both > transmission and reflection mode- for measuring true magnification? Hi Shalin, I used a USAF 1951 target for exactly that purpose. It has a mirror surface with a clear pattern on it. It appears there are several vendors for those. I got it from Edmund http://www.edmundoptics.com/ I believe the one that I used currently costs 111 Euros in Germany (cat. no. Y38-256). I switched work places and just reordered it, so I can't check the cat number on the slide itself. One note of caution: I had some trouble with a version of the slide that contains fluorescent material. The focal plain is so far away from the surface, that the working distance of my oil immersion objectives was too short to reach it. So you're better off with the one with the surface mirror and use that in reflection or transmission mode, as you plan anyway. Steffen -- --------------------------------------------------------------------------------------------------- Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Walter-Brendel-Zentrum (WBZ) Head of light microscopy Building location and address for courier, parcel services etc: Marchioninistr. 27, D-81366 München (Großhadern) Mailing address (for letters etc.): Marchioninistr. 15, D-81366 München |
«
Return to Confocal Microscopy List
|
1 view|%1 views
| Free forum by Nabble | Edit this page |