*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
Feulgen reaction should work, assuming it's not completely chewed up.
Julian
On 8/21/17 3:26 AM, Gelman, Laurent wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Hi everybody,
>
> We would like to stain DNA in fixed cells after denaturation with HCL, a treatment that breaks the double-strand structure. DAPI works very poorly, we are looking rather for non-intercalating dyes.
>
> Any experience?
>
> Many thanks in advance for your help,
>
> Very best regards,
>
> Laurent.
>
>
> Laurent Gelman
> Facility for Advanced Imaging and Microscopy
> Head Light Microscopy
> Friedrich Miescher Institute for Biomedical Research
> Maulbeerstrasse 66
> 4058 basel
> Switzerland
> +41 796187369
--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
349 Columbia Ave
Rock Hill, SC 29733
803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)
Research Website www.birdnest.org/smithj
Personal Website www.rociada-east.net
Locked
