Starch-stearic acid

[Lloyd Donaldson]

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Hi Alan

Periodic acid Schiff reagent (PAS) will stain starch grains with fluorescence in the red or far red. You might be able to distinguish it from the nile red.

Regards

Dr Lloyd Donaldson - Senior Scientist
Cellwall Biotechnology Centre
SCION - Next generation biomaterials
Te Papa Tipu Innovation Park, 49 Sala Street
Private Bag 3020, ROTORUA, New Zealand
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I have a student that wants to image starch-paste and stearic acid. The aim is to show the starch granules coated with stearic acid. We have tried FITC to stain the starch, but that gives a very high background. For the stearic acid we used Nile red.

All suggestions and comments will be appreciated.
TIA
Alan Hall


Laboratory for Microscopy & Microanalysis
Room1-33, Natural Sciences 2
University of Pretoria
Lynnwood Road
Pretoria
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