Stitching movies with non-matching Z planes?

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Ekaterina PAPUSHEVA-2 Ekaterina PAPUSHEVA-2
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Stitching movies with non-matching Z planes?

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Happy new year to everyone,

Does someone know a script that allows to stitch timelapse of Z-stacks with non-matching Z positions?  We have movies consisting of two parts, each part is taken with different Z-settings . Z step and number of planes are the same, but we had to shift the Z-stack start/end upwards as the sample migrated.

Grateful in advance!
Ekaterina

___

Ekaterina Papusheva

Manager of Bioimaging Facility

Institute of Science and Technology Austria

Phone  +43-(0)2243 9000-1043;
Mobile +43-(0)664 88509130
Fax    +43-(0)2243 9000-2000

Am Campus 1

A-3400 Klosterneuburg

Visit our website: www.ist.ac.at<http://www.ist.ac.at>


This message and any attachment (together "the Message") are confidential and intended only for the individual named. If you have received the Message in error you should not distribute or copy this Message. Please notify the sender immediately by e-mail if you have received this Message in error and delete the Message from your system. Correspondence via e-mail is for information purposes. IST Austria neither makes nor accepts legally binding statements unless explicitly otherwise agreed to the contrary.
Mario Emmenlauer Mario Emmenlauer
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Re: Stitching movies with non-matching Z planes?

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Hi,

if you have overlap in X, Y or Z between pairs of stacks, then
XuvTools can do that. Check it out at http://xuvtools.org its
free and open source software.

Cheers,

     Mario



On 01/04/2012 10:44 AM, Ekaterina PAPUSHEVA wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Happy new year to everyone,
>
> Does someone know a script that allows to stitch timelapse of Z-stacks with non-matching Z positions?  We have movies consisting of two parts, each part is taken with different Z-settings . Z step and number of planes are the same, but we had to shift the Z-stack start/end upwards as the sample migrated.
>
> Grateful in advance!
> Ekaterina
>
> ___
>
> Ekaterina Papusheva
>
> Manager of Bioimaging Facility
>
> Institute of Science and Technology Austria
>
> Phone  +43-(0)2243 9000-1043;
> Mobile +43-(0)664 88509130
> Fax    +43-(0)2243 9000-2000
>
> Am Campus 1
>
> A-3400 Klosterneuburg
>
> Visit our website: www.ist.ac.at<http://www.ist.ac.at>
>
>
> This message and any attachment (together "the Message") are confidential and intended only for the individual named. If you have received the Message in error you should not distribute or copy this Message. Please notify the sender immediately by e-mail if you have received this Message in error and delete the Message from your system. Correspondence via e-mail is for information purposes. IST Austria neither makes nor accepts legally binding statements unless explicitly otherwise agreed to the contrary.
>