Stray light in TIRF microscopy

[Martin Wessendorf-2]

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[Posted on behalf of Dr. Alexander Asanov.]

Dear colleagues,

Are you aware of quantitative data for the intensity of stray light in
TIRF microscopy? I found only one paper by A. Mattheyses and D. Axelrod,
2006, which quantitatively estimated the intensity of stray light to be
~10–15% relative to the intensity of the evanescent wave at the TIRF
interface [Mattheyses, A. L., and Axelrod D. Direct measurement of the
evanescent field profile produced by objective-based total internal
reflection fluorescence. J Biomed Opt. 2006 Jan-Feb;11(1):014006.]

There are two 2014 papers by Martin Oheim’s group that referenced most
of the literature and analyzed sources of stray light, but did not make
a quantitative comparison. [Brunstein M, Teremetz M, Hérault K, Tourain
C, Oheim M. Eliminating unwanted far-field excitation in objective-type
TIRF. Part I. identifying sources of nonevanescent excitation light.
Biophys J. 2014 Mar 4;106(5):1020-32.   Brunstein M, Hérault K, Oheim M.
Eliminating unwanted far-field excitation in objective-type TIRF. Part
II. combined evanescent-wave excitation and supercritical-angle
fluorescence detection improves optical sectioning. Biophys J. 2014 Mar
4;106(5):1044-56.]

Does anybody know additional published or unpublished data with
Quantitative comparison of the stray light with the evanescent wave?  
Thank you.

Best regards,
Alexander Asanov, Ph.D.
TIRF Labs
[hidden email]

--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[John Oreopoulos]

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Dear Alexander,

I observed the stray light phenomenon for through-the objective TIRF in this paper:

Oreopoulos, J. and C.M. Yip, Combined scanning probe and total internal reflection fluorescence microscopy. Methods, 2008. 46(1): p. 2-10.

See Figure 4.

However, in a very similar instrumental setup, the authors of this paper did not:

Sarkar, A., R.B. Robertson, and J.M. Fernandez, Simultaneous atomic force microscope and fluorescence measurements of protein unfolding using a calibrated evanescent wave. Proceedings of the National Academy of Sciences of the United States of America, 2004. 101(35): p. 12882-12886.

Other papers from the Oheim group that might interest you:

Schapper, F., J.T. Goncalves, and M. Oheim, Fluorescence imaging with two-photon evanescent wave excitation. European Biophysics Journal with Biophysics Letters, 2003. 32(7): p. 635-643.

Oheim, M. and F. Schapper, Non-linear evanescent-field imaging. Journal of Physics D-Applied Physics, 2005. 38(10): p. R185-R197.


Cheers,

John Oreopoulos



On 2016-07-08, at 4:10 PM, Martin Wessendorf wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> [Posted on behalf of Dr. Alexander Asanov.]
>
> Dear colleagues,
>
> Are you aware of quantitative data for the intensity of stray light in TIRF microscopy? I found only one paper by A. Mattheyses and D. Axelrod, 2006, which quantitatively estimated the intensity of stray light to be ~10–15% relative to the intensity of the evanescent wave at the TIRF interface [Mattheyses, A. L., and Axelrod D. Direct measurement of the evanescent field profile produced by objective-based total internal reflection fluorescence. J Biomed Opt. 2006 Jan-Feb;11(1):014006.]
>
> There are two 2014 papers by Martin Oheim’s group that referenced most of the literature and analyzed sources of stray light, but did not make a quantitative comparison. [Brunstein M, Teremetz M, Hérault K, Tourain C, Oheim M. Eliminating unwanted far-field excitation in objective-type TIRF. Part I. identifying sources of nonevanescent excitation light. Biophys J. 2014 Mar 4;106(5):1020-32.   Brunstein M, Hérault K, Oheim M. Eliminating unwanted far-field excitation in objective-type TIRF. Part II. combined evanescent-wave excitation and supercritical-angle fluorescence detection improves optical sectioning. Biophys J. 2014 Mar 4;106(5):1044-56.]
>
> Does anybody know additional published or unpublished data with Quantitative comparison of the stray light with the evanescent wave?  Thank you.
>
> Best regards,
> Alexander Asanov, Ph.D.
> TIRF Labs
> [hidden email]
>
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]

[Armstrong, Brian]

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*****

Hi John, pardon my laziness, but what advantage does 2P evanescent wave microscopy have over "1P"?
Thanks,

Brian Armstrong PhD
Associate Research Professor
Developmental and Stem Cell Biology
Diabetes and Metabolic Diseases
Director, Light Microscopy Core
Beckman Research Institute, City of Hope



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of John Oreopoulos
Sent: Monday, July 11, 2016 9:05 PM
To: [hidden email]
Subject: Re: Stray light in TIRF microscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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*****

Dear Alexander,

I observed the stray light phenomenon for through-the objective TIRF in this paper:

Oreopoulos, J. and C.M. Yip, Combined scanning probe and total internal reflection fluorescence microscopy. Methods, 2008. 46(1): p. 2-10.

See Figure 4.

However, in a very similar instrumental setup, the authors of this paper did not:

Sarkar, A., R.B. Robertson, and J.M. Fernandez, Simultaneous atomic force microscope and fluorescence measurements of protein unfolding using a calibrated evanescent wave. Proceedings of the National Academy of Sciences of the United States of America, 2004. 101(35): p. 12882-12886.

Other papers from the Oheim group that might interest you:

Schapper, F., J.T. Goncalves, and M. Oheim, Fluorescence imaging with two-photon evanescent wave excitation. European Biophysics Journal with Biophysics Letters, 2003. 32(7): p. 635-643.

Oheim, M. and F. Schapper, Non-linear evanescent-field imaging. Journal of Physics D-Applied Physics, 2005. 38(10): p. R185-R197.


Cheers,

John Oreopoulos



On 2016-07-08, at 4:10 PM, Martin Wessendorf wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> [Posted on behalf of Dr. Alexander Asanov.]
>
> Dear colleagues,
>
> Are you aware of quantitative data for the intensity of stray light in TIRF microscopy? I found only one paper by A. Mattheyses and D. Axelrod, 2006, which quantitatively estimated the intensity of stray light to be ~10-15% relative to the intensity of the evanescent wave at the TIRF interface [Mattheyses, A. L., and Axelrod D. Direct measurement of the evanescent field profile produced by objective-based total internal reflection fluorescence. J Biomed Opt. 2006 Jan-Feb;11(1):014006.]
>
> There are two 2014 papers by Martin Oheim's group that referenced most of the literature and analyzed sources of stray light, but did not make a quantitative comparison. [Brunstein M, Teremetz M, Hérault K, Tourain C, Oheim M. Eliminating unwanted far-field excitation in objective-type TIRF. Part I. identifying sources of nonevanescent excitation light. Biophys J. 2014 Mar 4;106(5):1020-32.   Brunstein M, Hérault K, Oheim M. Eliminating unwanted far-field excitation in objective-type TIRF. Part II. combined evanescent-wave excitation and supercritical-angle fluorescence detection improves optical sectioning. Biophys J. 2014 Mar 4;106(5):1044-56.]
>
> Does anybody know additional published or unpublished data with Quantitative comparison of the stray light with the evanescent wave?  Thank you.
>
> Best regards,
> Alexander Asanov, Ph.D.
> TIRF Labs
> [hidden email]
>
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]

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[Craig Brideau]

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*****

In theory the 2p requirement will further limit the effect of the field
closer to the surface.

Craig
On Jul 12, 2016 9:37 AM, "Armstrong, Brian" <[hidden email]> wrote:

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi John, pardon my laziness, but what advantage does 2P evanescent wave
microscopy have over "1P"?
Thanks,

Brian Armstrong PhD
Associate Research Professor
Developmental and Stem Cell Biology
Diabetes and Metabolic Diseases
Director, Light Microscopy Core
Beckman Research Institute, City of Hope



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of John Oreopoulos
Sent: Monday, July 11, 2016 9:05 PM
To: [hidden email]
Subject: Re: Stray light in TIRF microscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Alexander,

I observed the stray light phenomenon for through-the objective TIRF in
this paper:

Oreopoulos, J. and C.M. Yip, Combined scanning probe and total internal
reflection fluorescence microscopy. Methods, 2008. 46(1): p. 2-10.

See Figure 4.

However, in a very similar instrumental setup, the authors of this paper
did not:

Sarkar, A., R.B. Robertson, and J.M. Fernandez, Simultaneous atomic force
microscope and fluorescence measurements of protein unfolding using a
calibrated evanescent wave. Proceedings of the National Academy of Sciences
of the United States of America, 2004. 101(35): p. 12882-12886.

Other papers from the Oheim group that might interest you:

Schapper, F., J.T. Goncalves, and M. Oheim, Fluorescence imaging with
two-photon evanescent wave excitation. European Biophysics Journal with
Biophysics Letters, 2003. 32(7): p. 635-643.

Oheim, M. and F. Schapper, Non-linear evanescent-field imaging. Journal of
Physics D-Applied Physics, 2005. 38(10): p. R185-R197.


Cheers,

John Oreopoulos



On 2016-07-08, at 4:10 PM, Martin Wessendorf wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> [Posted on behalf of Dr. Alexander Asanov.]
>
> Dear colleagues,
>
> Are you aware of quantitative data for the intensity of stray light in

TIRF microscopy? I found only one paper by A. Mattheyses and D. Axelrod,
2006, which quantitatively estimated the intensity of stray light to be
~10-15% relative to the intensity of the evanescent wave at the TIRF
interface [Mattheyses, A. L., and Axelrod D. Direct measurement of the
evanescent field profile produced by objective-based total internal
reflection fluorescence. J Biomed Opt. 2006 Jan-Feb;11(1):014006.]
>
> There are two 2014 papers by Martin Oheim's group that referenced most of
the literature and analyzed sources of stray light, but did not make a
quantitative comparison. [Brunstein M, Teremetz M, Hérault K, Tourain C,
Oheim M. Eliminating unwanted far-field excitation in objective-type TIRF.
Part I. identifying sources of nonevanescent excitation light. Biophys J.
2014 Mar 4;106(5):1020-32.   Brunstein M, Hérault K, Oheim M. Eliminating
unwanted far-field excitation in objective-type TIRF. Part II. combined
evanescent-wave excitation and supercritical-angle fluorescence detection
improves optical sectioning. Biophys J. 2014 Mar 4;106(5):1044-56.]
>
> Does anybody know additional published or unpublished data with
Quantitative comparison of the stray light with the evanescent wave?  Thank
you.

>
> Best regards,
> Alexander Asanov, Ph.D.
> TIRF Labs
> [hidden email]
>
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]

---------------------------------------------------------------------
*SECURITY/CONFIDENTIALITY WARNING:
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entity to which they are addressed. This communication may contain
information that is privileged, confidential, or exempt from disclosure
under applicable law (e.g., personal health information, research data,
financial information). Because this e-mail has been sent without
encryption, individuals other than the intended recipient may be able to
view the information, forward it to others or tamper with the information
without the knowledge or consent of the sender. If you are not the intended
recipient, or the employee or person responsible for delivering the message
to the intended recipient, any dissemination, distribution or copying of
the communication is strictly prohibited. If you received the communication
in error, please notify the sender immediately by replying to this message
and deleting the message and any accompanying files from your system. If,
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communications via e-mail, please reply to this message and inform the
sender that you do not wish to receive further e-mail from the sender.
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