T-PMT image moves from left to right (LSM710)

[Shimo]

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Recently I had a problem with LSM710's Real Time PC communication problem.
The RTC reset button or restarting the system didn't solve the problem.
To solve the problem, I had to open up the black box, reseat the NIC card
and restart the system.

The communication problem has been solved this way, but the troubleshooting
caused another problem.
When I image my sample with T-PMT (transmission light image), only T-PMT
image moves from left to right.
Reflective fluorescent images are perfectly fine.

In the Live imaging mode T-PMT image keeps moving from left to right as the
sample is being scanned.
When the image is gone to the right edge, it will come back from the left
after a while.
The image seems to move by a fixed increment after every frame of scanning.

Has anybody had this kind of problem before?
I have sent email to Zeiss tech support but no reply so far.

Thank you.

[jlribas]

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Dear Tsutomu,

We have the same problem and no idea (for more than 5 years). The screen
movement is aproximately 10% of the screen. It is intermitenly and once
it happens you need to restart the PC (Windows).

All the best

Juan Luis


El 17/07/2017 a las 19:51, Tsutomu Shimotori escribió:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Recently I had a problem with LSM710's Real Time PC communication problem.
> The RTC reset button or restarting the system didn't solve the problem.
> To solve the problem, I had to open up the black box, reseat the NIC card
> and restart the system.
>
> The communication problem has been solved this way, but the troubleshooting
> caused another problem.
> When I image my sample with T-PMT (transmission light image), only T-PMT
> image moves from left to right.
> Reflective fluorescent images are perfectly fine.
>
> In the Live imaging mode T-PMT image keeps moving from left to right as the
> sample is being scanned.
> When the image is gone to the right edge, it will come back from the left
> after a while.
> The image seems to move by a fixed increment after every frame of scanning.
>
> Has anybody had this kind of problem before?
> I have sent email to Zeiss tech support but no reply so far.
>
> Thank you.
>

--
Juan Luis Ribas
Servicio de Microscopía
Centro de Investigación, Tecnología e Innovación
Universidad de Sevilla
Av. Reina Mercedes 4b
41012 Sevilla

Tfnos: 954559983 // 628220681 // 12068

http://investigacion.us.es/scisi/sgi/servicios/microscopia/equipamiento

[Moulding, Dale]

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Dear Tsutomu,

I've had the same problem, LSM710 with Zen 2009. T-PMT image was shifted horizontally, with about 20% of the image switched from the right to left side. If I remember correctly turning off bidirectional scan always fixed this. All reflected channels were fine.
Fortunately the problem has gone away now, about the same time Zeiss swapped our old vista PC for a newer model running Windows 7. (Coincidence?)
Good luck getting to the bottom of this,
Dale



Dale Moulding PhD
ICH Imaging Facility,
Room W2.06, BDRC office,
UCL Great Ormond Street Institute of Child Health,
30 Guilford St,
London WC1N 1EH
http://www.ucl.ac.uk/ich/core-scientific-facilities-centres/confocal-microscopy



-------- Original message --------
From: Tsutomu Shimotori <[hidden email]>
Date: 17/07/2017 19:02 (GMT+00:00)
To: [hidden email]
Subject: T-PMT image moves from left to right (LSM710)

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Recently I had a problem with LSM710's Real Time PC communication problem.
The RTC reset button or restarting the system didn't solve the problem.
To solve the problem, I had to open up the black box, reseat the NIC card
and restart the system.

The communication problem has been solved this way, but the troubleshooting
caused another problem.
When I image my sample with T-PMT (transmission light image), only T-PMT
image moves from left to right.
Reflective fluorescent images are perfectly fine.

In the Live imaging mode T-PMT image keeps moving from left to right as the
sample is being scanned.
When the image is gone to the right edge, it will come back from the left
after a while.
The image seems to move by a fixed increment after every frame of scanning.

Has anybody had this kind of problem before?
I have sent email to Zeiss tech support but no reply so far.

Thank you.

[Claire Brown]

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Is it a small periodic left and right movement? We had that problem on our 510 T-PMT years back. It was electrical interference in the PMT/cabling.
We shielded the PMT with chicken wire and the TPMT cable with thick tin foil. The problem went away.

You can test this by moving the cable slightly and see if the pattern in the movement is sensitive to the position of the cable. If it is it is likely electrical interference.

Good luck!

Claire

[Christian Feldhaus]

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Hi,

we had a similar problem with our T-PMT some time ago on our LSM 780.
Pixel timing information coming from the T-PMT to the RTC to correlate
T-PMT signal with the other detectors seemed to be corrupted. As far as
I remember the engineer rewired/ reconfigured all the CAN connections
and that solved the problem.

Best,

Christian


On 17.07.2017 19:51, Tsutomu Shimotori wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Recently I had a problem with LSM710's Real Time PC communication problem.
> The RTC reset button or restarting the system didn't solve the problem.
> To solve the problem, I had to open up the black box, reseat the NIC card
> and restart the system.
>
> The communication problem has been solved this way, but the troubleshooting
> caused another problem.
> When I image my sample with T-PMT (transmission light image), only T-PMT
> image moves from left to right.
> Reflective fluorescent images are perfectly fine.
>
> In the Live imaging mode T-PMT image keeps moving from left to right as the
> sample is being scanned.
> When the image is gone to the right edge, it will come back from the left
> after a while.
> The image seems to move by a fixed increment after every frame of scanning.
>
> Has anybody had this kind of problem before?
> I have sent email to Zeiss tech support but no reply so far.
>
> Thank you.

--
Christian Liebig, PhD
Light Microscopy Facility
MPI for Developmental Biology

Spemannstrasse 35
72076 Tübingen
Germany

Tel.: ++49 7071 601443